Xia Wu

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Name: 吴霞; Xia Wu

Organization: Key Laboratory of Colloid and Interface Chemistry (Shandong University) , China

Department: School of Chemistry and Chemical Engineering

Title: Professor(PhD)

TOPICS

Analytical Chemistry

Polymer

Stimuli Responsive Polymers PH Responsive Polymers Stimuli Responsive Polymers PH Responsive Polymers

Chemicals
References

Silver nanoprisms-based Tb(III) fluorescence sensor for highly selective detection of dopamine

Co-reporter:Jin Shen, Chongmei Sun, Xia Wu

Talanta 2017 Volume 165() pp:369-376

Publication Date(Web):1 April 2017

DOI:10.1016/j.talanta.2016.12.073

•Dopamine fluorescence sensor was constructed based on SEF of silver nanoprisms.•Anisotropic silver nanoprisms could remarkably enhanced Tb(III) luminescence.•Acetate was as a spacer for AgNPrs-based SEF and a recognition group for dopamine.Dopamine (DA) is one of catecholamines and related to several neurological diseases. The selective determination for DA against other catecholamines is crucial in clinical diagnoses. In this work, a simple and reliable Tb(III)-based fluorescence sensor was constructed for the highly selective and sensitive detection of DA. Silver nanoprisms (AgNPrs) with suitable localized surface plasmon resonance bands were controllably synthesized to act as optimal platforms for surface enhanced fluorescence (SEF), while acetate was adopted to be a distance adjusting spacer for SEF and a recognizing component for DA. A fluorescence-enhanced Tb(III) composite sensor via the synergistic action of AgNPrs and acetate was finally obtained. The fluorescence signal was in proportion to the dopamine concentration in the range of 0.6–100 nM (R2=0.9987) with a detection limit (S/N =3) of 0.22 nM. Furthermore, the method exhibited good selectivity to DA among some amino acids, ions, other neurotransmitters, etc.

A highly selective and sensitive fluorescent nanosensor for dopamine based on formate bridged Tb(III) complex and silver nanoparticles

Co-reporter:Huihui Li;Jin Shen;Rongwei Cui;Chongmei Sun;Yanyan Zhao;Na Li;Bo Tang

Analyst (1876-Present) 2017 vol. 142(Issue 22) pp:4240-4246

Publication Date(Web):2017/11/06

DOI:10.1039/C7AN00961E

Highly selective determination of dopamine (DA) over other catecholamines is an urgent need for the precise diagnosis and therapy of DA related diseases. Herein, a new formate-bridged Tb(III)-complex and silver nanoparticles (AgNPs) enhanced fluorescent nanosensor was constructed. HCOO− acted as a co-ligand of Tb(III) and also as a linker between the Tb(III) complex and AgNPs and more readily combined with the primary amine of DA than with epinephrine (EP). The formate-bridged action strengthened AgNPs-based surface enhanced fluorescence of the Tb3+-DA complex and improved the selectivity towards DA. Under neutral buffer conditions, the detection limit for the assay of DA was down to 0.15 nM (S/N = 3) with a linear range from 0.5 nM to 100 nM (R2 = 0.9978). Furthermore, the nanosensor could successfully distinguish DA from EP.

Study on the interaction of berberine with nucleic acids in the presence of silver nanoparticles, and the fluorometric determination of nucleic acids

Co-reporter:Yanyan Zhao, Haiping Zhou, Jin Shen, Minqin Wang and Xia Wu

RSC Advances 2016 vol. 6(Issue 35) pp:29612-29618

Publication Date(Web):17 Mar 2016

DOI:10.1039/C6RA02346K

In this paper, the metal-enhanced fluorescence effect of AgNPs to berberine (BER) was found and applied in the sensitive determination of nucleic acids. Under optimum conditions, the enhanced fluorescence intensity of the system exhibited a linear response with concentrations of nucleic acids in the range of 4.0 × 10−8 to 6.0 × 10−6 g mL−1 for fish sperm DNA (fsDNA) and 1.0 × 10−8 to 4.0 × 10−6 g mL−1 for calf thymus DNA (ctDNA). Their corresponding detection limits (S/N = 3) are 21 ng mL−1 and 7.9 ng mL−1. This method had been successfully used to detect plasmid DNA in actual samples. In addition, the results of this study on the interaction mechanism of the system evidenced that the partial intercalation binding action between BER and ctDNA was strengthened by AgNPs. The ctDNA could induce the aggregation of AgNPs and provide an appropriate distance for BER and AgNPs, which resulted in the enhancement of the fluorescence intensity and the anti-photobleaching activity of BER.

Fluorescence enhancement of fisetin by silver nanoparticles with cetyltrimethyl ammonium bromide micelles

Co-reporter:Xiaodan Liu and Xia Wu

RSC Advances 2015 vol. 5(Issue 10) pp:7433-7439

Publication Date(Web):09 Dec 2014

DOI:10.1039/C4RA12726A

In this work, in situ synthesized cetyltrimethyl ammonium bromide micelles-capped silver nanoparticles were used to sensitively and directly detect fisetin. Fisetin molecules solubilize in the Stern layer of cetyltrimethyl ammonium bromide micelles and mainly bind to the head group of the micelles by electrostatic interaction and hydrophobic effect. Silver nanoparticles also solubilize in cetyltrimethyl ammonium bromide micelles and form the micelles-capped silver nanoparticles. The suitable distances between fisetin molecules and silver nanoparticles for the fluorescence enhancement are provided by the micelles. The synergistic action of the solubilization and sensitization of micelles and metal-enhanced fluorescence of silver nanoparticles promote the fluorescence enhancement of the system. Under optimized conditions, the fluorescence intensity of the system exhibits a linear response with the concentrations of fisetin in the range from 5.0 × 10−8 mol L−1 to 7.0 × 10−6 mol L−1 and the detection limit (S/N = 3) is 1.5 × 10−9 mol L−1. Compared to the other methods, the developed method has higher sensitivity. Moreover, the method has been successfully used to detect fisetin in actual samples.

Silver nanoparticles-enhanced rare earth co-luminescence effect of Tb(III)–Y(III)–dopamine system

Co-reporter:Huihui Li, Xia Wu

Talanta 2015 Volume 138() pp:203-208

Publication Date(Web):1 June 2015

DOI:10.1016/j.talanta.2015.02.023

•Silver nanoparticles enhanced co-luminescence of Tb3+–Y3+–DA in aqueous solution.•The proposed method was sensitive and anti-photobleaching.•The MEF-based method for the determination of trace amount of DA was proposed.It was found that silver nanoparticles (AgNPs) could enhance co-luminescence effect of rare earths ions Tb3+ and Y3+. Based on this, a sensitive fluorescence detection method for the determination of dopamine (DA) was proposed. Moreover, the detection limit for DA was very low (down to nM). This is because DA can remarkably enhance the luminescence intensity of the Tb3+ ion by Y3+ in the colloidal solution of AgNPs, forming a new co-luminescence system. Furthermore, based on the metal enhanced fluorescence (MEF), AgNPs can sensitize the co-luminescence effect of the complex of Tb3+–Y3+–DA. In a neutral buffer solution (pH 7.50), the luminescence intensity of the system was linearly related to the concentration of DA in the range of 2.0–100 nM, with a limit of detection as low as 0.57 nM. The proposed method was applied for the determination of DA in dopamine hydrochloride injections and human serum samples with good accuracy and satisfactory recovery.Silver nanoparticles could enhance rare earth co-luminescence of Tb(III)–Y(III)–DA in neutral aqueous solution. The sensitive and anti-photobleaching method for the determination of DA was developed.

Fluorescence enhancement effect of Eu(III)–thenoyltrifluoroacetone–cetyltrimethyl ammonium bromide in water-dissolved organic matter extracted from wheat straw

Co-reporter:Fei Huang, Fanhui Meng, Mengdi Fan, Yanyan Zhao, Xia Wu, Lin Shen

Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy 2015 Volume 151() pp:302-307

Publication Date(Web):5 December 2015

DOI:10.1016/j.saa.2015.06.090

•3D-EEM of water-dissolved organic matter extracted from wheat straw is studied.•DOM-WS can significantly enhance the fluorescence of Eu(III)–TTA–CTAB.•Adding DOM-WS can reduce TTA dosage for the detection of Eu(III).The fluorescence spectral characteristics of water-dissolved organic matter extracted from wheat straw (DOM-WS) were studied using three-dimensional excitation–emission matrix (3D-EEM) fluorescence spectroscopy. The results indicated that 3D-EEM spectra of DOM-WS showed four different fluorophores: humic-like, visible fulvic-like, UV fulvic-like and protein-like substances. It is interesting that DOM-WS can obviously enhance the fluorescence intensity of Eu(III)–thenoyltrifluoroacetone–cetyltrimethyl ammonium bromide system. On the basis of this study, a new fluorescence method for the determination of trace amounts of Eu(III) was developed. Under the optimal conditions, the enhanced fluorescence intensity was in proportion to the concentration of Eu(III) in the range of 8.0 × 10−8–8.0 × 10−7 mol/L. The detection limit (S/N = 3) was 1.1 × 10−9 mol/L. This method was applied to the analysis of Eu(III) concentration in standard sample and obtained satisfactory results. It may be a new way to use wheat straw effectively.DOM from wheat straw can dramatically increase the fluorescence of Eu(III)–TTA–CTAB. The strong red fluorescence of Eu(III)–TTA–CTAB–DOM-WS can even be observed by naked eyes under a common UV lamp irradiation. Four emission peaks located at 537 nm, 592 nm, 614 nm and 653 nm, corresponding to 5D1 → 7F1, 5D0 → 7F1, 5D0 → 7F2 and 5D0 → 7F3 of Eu(III) characteristic transition, respectively.

Synchronous fluorescence determination of ferulic acid with Ce(IV) and sodium tripolyphosphate

Co-reporter:F Meng;P Liu;F Huang;L Wang;X Wu;L Shen

Luminescence 2014 Volume 29( Issue 3) pp:243-247

Publication Date(Web):

DOI:10.1002/bio.2535

ABSTRACT

In this study, a synchronous fluorescence detection method for ferulic acid (FA) is proposed based on a redox reaction between FA and Ce(IV) sulfate in dilute sulfuric acid medium at room temperature. It was found that FA could reduce Ce(IV) to Ce(III) in acidic medium, and sodium tripolyphosphate could further enhance the intrinsic fluorescence of the Ce(III) produced. The enhanced extent of synchronous fluorescence intensity was in proportion to the concentration of FA over the range 3.0 × 10^-8 to 1.0 × 10^-5 mol/L. The corresponding limit of determination (S/N = 3) was 1.3 × 10^-8 mol/L. The proposed method was applied to the determination of sodium ferulate for injection sample with satisfactory results. Copyright © 2013 John Wiley & Sons, Ltd.

Fluorescence enhancement of quercetin complexes by silver nanoparticles and its analytical application

Co-reporter:Ping Liu, Liangliang Zhao, Xia Wu, Fei Huang, Minqin Wang, Xiaodan Liu

Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy 2014 Volume 122() pp:238-245

Publication Date(Web):25 March 2014

DOI:10.1016/j.saa.2013.11.055

•The system with AgNPs exhibited stronger luminescence and higher photostability.•The Qu–AgNPs complex was used as a fluorescence probe for nucleic acids detection.•The Qu–AgNPs system was applied in fluorescence image analysis of protoplasts.It is found that the plasmon effect of silver nanoparticles (AgNPs) helps to enhance the fluorescence intensity of the quercetin (Qu) and nucleic acids system. Qu exhibited strong fluorescence enhancement when it bound to nucleic acids in the presence of AgNPs. Based on this, a sensitive method for the determination of nucleic acids was developed. The detection limits for the nucleic acids (S/N = 3) were reduced to the ng mL−1 level. The interaction mechanism of the AgNPs-fish sperm DNA (fsDNA)–Qu system was also investigated in this paper. This complex system of Qu and AgNPs was also successfully used for the detection of nucleic acids in agarose gel electrophoresis analysis. Preliminary results indicated that AgNPs also helped to improve sensitivity in the fluorescence image analysis of Qu combined with cellular contents in Arabidopsis thaliana protoplasts.

Resonance light scattering technique for determination of polychlorinated biphenyls with silver nanoparticles

Co-reporter:Fengju Zhang;Jinhua Zhan

Luminescence 2011 Volume 26( Issue 6) pp:656-661

Publication Date(Web):

DOI:10.1002/bio.1292

ABSTRACT

In this paper, a simple and novel method for the determination of polychlorinated biphenyls (PCBs), using silver nanoparticles (AgNPs) as a resonance light scattering (RLS) probe, is proposed. Under optimized conditions, there existed linear relationships between the enhancing RLS intensity of the system and the concentrations of PCBs in the range 8.0 × 10⁻⁸−1.0 × 10⁻⁶ g mL⁻¹ for 2,4,4′-trichlorbiphenyl (PCB28), 9.0 × 10⁻⁸−1.0 × 10⁻⁶ g mL⁻¹ for 2,2′,5,5′-tetrachlorbiphenyl (PCB52) and 4.0 × 10⁻⁸−1.0 × 10⁻⁶ g mL⁻¹ for 3,3′,4,4′-tetrachlorobiphenyl (PCB77). The corresponding detection limits (S/N = 3) were 2.6 × 10⁻⁸ g mL⁻¹ for PCB28, 3.3 × 10⁻⁸ g mL⁻¹ for PCB52 and 6.3 × 10⁻⁹ g mL⁻¹ for PCB77, respectively. Finally, the mechanism of RLS enhancement was also studied. The results indicated that PCBs were adsorbed on the surface of AgNPs to form larger AgNP–PCB aggregates, resulting in the RLS enhancement of the system. Copyright © 2011 John Wiley & Sons, Ltd.

Silver nanoparticles fluorescence enhancement effect for determination of nucleic acids with kaempferol–Al(III)

Co-reporter:Yinghua Cao, Xia Wu, Minqin Wang

Talanta 2011 Volume 84(Issue 4) pp:1188-1194

Publication Date(Web):30 May 2011

DOI:10.1016/j.talanta.2011.03.031

Nucleic acids can greatly enhance fluorescence intensity of the kaempferol (Km)–Al(III) system in the presence of silver nanoparticles (AgNPs). Based on this, a novel method for the determination of nucleic acids is proposed. Under studied conditions, there are linear relationships between the extent of fluorescence enhancement and the concentration of nucleic acids in the range of 5.0 × 10−9 to 2.0 × 10−6 g mL−1 for fish sperm DNA (fsDNA), 7.0 × 10−9 to 2.0 × 10−6 g mL−1 for salmon sperm DNA (smDNA) and 2.0 × 10−8 to 3.0 × 10−6 g mL−1 for yeast RNA (yRNA), and their detection limits are 2.5 × 10−9 g mL−1, 3.2 × 10−9 g mL−1 and 7.3 × 10−9 g mL−1, respectively. Samples were satisfactorily determined. And the system of Km–Al(III)–AgNPs was used as a fluorescence staining reagent for sensitive DNA detection by DNA pattern of agarose gel electrophoresis analysis. The results indicate that the fluorescence enhancement should be attributed to the formation of Km–Al(III)–AgNPs–nucleic acids aggregations through electrostatic attraction and adsorption bridging action of Al(III) and the surface-enhanced fluorescence effect of AgNPs.

Fluorescence Enhancement Effect for the Determination of Polychlorinated Biphenyls with Bovine Serum Albumin

Co-reporter:Fengju Zhang;Jinhua Zhan

Journal of Fluorescence 2011 Volume 21( Issue 5) pp:

Publication Date(Web):2011/09/01

DOI:10.1007/s10895-011-0880-9

A sensitive and selective method for the trace determination of 3, 3’, 4, 4’-tetrachlorobiphenyl (PCB77) by using bovine serum albumin (BSA) as a fluorescence probe was introduced. Under optimum conditions, the enhanced fluorescence intensity was proportional to the concentration of polychlorinated biphenyls in the range of 8.9 × 10−8–5.0 × 10−6 mol L−1 for PCB77, and 5.0 × 10−7–5.0 × 10−6 mol L−1 for 2, 2’, 5, 5’-tetrachlorbiphenyl (PCB52). The detection limits (S/N = 3) of PCB77 and PCB52 were 2.6 × 10−8 mol L−1 and 2.9 × 10−7 mol L−1, respectively. Furthermore, the fluorescence enhancement mechanism was discussed in detail. Results indicated that fluorescence enhancement of the system originated from the formation of BSA-PCBs complexes. In addition, PCBs were mainly bound to the tyrosine residues in BSA molecules.

Nucleic acids determination using the complex of eriochrome black T and silver nanoparticles in a resonance light scattering technique

Co-reporter:Haiping Zhou, Xia Wu, Fanhui Meng, Jinghe Yang, Minqin Wang

Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy 2011 Volume 78(Issue 2) pp:681-686

Publication Date(Web):February 2011

DOI:10.1016/j.saa.2010.11.048

A novel method for the determination of nucleic acids by using silver nanoparticle (AgNPs)–eriochrome black T (EBT) as a resonance light scattering (RLS) probe has been developed. Under optimum conditions, there are linear relationships between the quenching extent of RLS intensity and the concentration of nucleic acids in the range of 4.0 × 10−9–4.0 × 10−7, 4.0 × 10−7–1.6 × 10−6 g mL−1 for fish sperm DNA (fsDNA) and 4.0 × 10−8–2.0 × 10−6 g mL−1 for yeast RNA (yRNA). Their detection limits (S/N = 3) are 2.0 ng mL−1 and 21 ng mL−1, respectively. The results indicate that AgNPs can form wirelike aggregates and nanoslices in the presence of the EBT. Whereas, when nucleic acids are added into the AgNPs–EBT system, the dynamic balance of AgNPs–EBT system is destroyed and the nanoparticles undergo dispersion again, leading to the RLS intensity of AgNPs–EBT system quenching. Meanwhile, the conformation of fsDNA is changed by the synergistic effect of AgNPs and EBT.

Protein determination using methylene blue in a synchronous fluorescence technique

Co-reporter:Xiaoyu Liu, Xia Wu, Jinghe Yang

Talanta 2010 Volume 81(Issue 3) pp:760-765

Publication Date(Web):15 May 2010

DOI:10.1016/j.talanta.2010.01.014

A new method for detecting protein by synchronous fluorescence enhancement was developed, based on the combination of near infrared (NIR) fluorescence and the dedimerization phenomenon of methylene blue (MB). Under analytical conditions, there are linear relationships between the enhancing extent of synchronous fluorescence of MB–sodium dodecyl benzene sulfonate (SDBS)–protein at 667 nm and the concentration of protein in the range of 8.0 × 10−8–4.0 × 10−5 g mL−1 for bovine serum albumin (BSA), 1.0 × 10−7–3.5 × 10−5 g mL−1 for egg albumin (EA). The detection limits (S/N = 3) of BSA and EA are 8.9 ng mL−1 and 10.0 ng mL−1, respectively. The fluorescence enhancement mechanism is discussed in detail. Results from multiple techniques indicate that the fluorescence enhancement of the system originates from the hydrophobic microenvironment provided by BSA and SDBS, and the formation of an MB–SDBS–BSA complex, as well as the deaggregation of some MB dimer.

Fluorescence Enhancement of the Silver Nanoparticales – Curcumin - Cetyltrimethylammonium Bromide-nucleic Acids System and its Analytical Application

Co-reporter:Haiping Zhou;Wei Xu;Jinghe Yang;Qiuxia Yang

Journal of Fluorescence 2010 Volume 20( Issue 4) pp:843-850

Publication Date(Web):2010 July

DOI:10.1007/s10895-010-0627-z

It is found that silver nanoparticles (AgNPs) can further enhance the fluorescence intensity of curcumin (CU) - cetyltrimethylammonium bromide (CTAB) – nucleic acids and improve its anti-photobleaching activity. Under optimum conditions, the enhanced fluorescence intensity is proportion to the concentration of nucleic acids in the range of 2.0 × 10−8–1.0 × 10−6 g mL−1 for fish sperm DNA (fsDNA), 2.0 × 10−8–1.0 × 10−6 g mL−1 for calf thymus DNA (ctDNA), 1.0 × 10−8–1.0 × 10−6 g mL−1 for yeast RNA (yRNA), and their detection limits (S/N = 3) are 8.0 ng mL−1, 10.5 ng mL−1 and 5.8 ng mL−1, respectively. This method is used for determining the concentration of DNA in actual sample with satisfactory results. The interaction mechanism is also studied.

The Fluorescence Enhancement of the Protein Adsorbed on the Surface of Ag Nanoparticle

Co-reporter:Changxia Sun;Honghong Ding;Liangliang Zhao;Fei Wang

Journal of Fluorescence 2009 Volume 19( Issue 1) pp:111-117

Publication Date(Web):2009 January

DOI:10.1007/s10895-008-0392-4

The fluorescence enhancement of the BSA adsorbed on the surface of Ag nanoparticles is reported, where non-fluorescent collagen is used as the separator between the BSA and Ag nanoparticles. The study indicates that Ag nanoparticles can enhance the fluorescence of the BSA, especially the fluorescence of the tyrosine residues with lower quantum. Three types of Ag nanoparticles are evaluated including Ag island film, Ag colloids and fractal Ag electrode. Of them Ag island film is the best. The investigation suggests that the fluorescence enhancement of the BSA is related to the adsorption of the BSA on the surface of Ag island film through the hydrophobic interaction, while the collagen can promote the adsorption of the BSA on the surface of Ag island film and change its conformation, resulting in the interaction between BSA and Ag island film.

The fluorescence enhancement of quercetinnucleic acid system and the analytical application

Co-reporter:Yuan Liu;Haiping Zhou;Xiaoyu Liu;Fengju Zhang;Jinghe Yang

Luminescence 2009 Volume 24( Issue 6) pp:416-421

Publication Date(Web):

DOI:10.1002/bio.1128

Abstract

Nucleic acid can greatly enhance the fluorescence intensity of quercetin in HMTA-HCl (pH 5.5) buffer. The enhanced intensity is in proportion to the concentration of nucleic acids in the range 5.0 × 10⁻⁹ to 1.0 × 10⁻⁶g/mL for fsDNA, 5.0 × 10⁻⁹ to 7.0 × 10⁻⁷g/mL for ctDNA and 5.0 × 10⁻⁹ to 1.0 × 10⁻⁶g/mL for yRNA, and their detection limits (S/N = 3) are 3.5 × 10⁻⁹, 7.8 × 10⁻¹⁰and 2.6 × 10⁻⁹g/mL, respectively. In comparison with most reported fluorescent probes for the determination of nucleic acids, the proposed probe has higher sensitivity and lower toxicity. The interaction investigation indicates that quercetin binds with double-strand DNA in groove binding mode, resulting in fluorescence enhancement of this system. Copyright © 2009 John Wiley & Sons, Ltd.

Study on the interaction of nucleic acids with silver nanoparticles—Al(III) by resonance light scattering technique and its analytical application

Co-reporter:Haiping Zhou, Xia Wu, Jinghe Yang

Talanta 2009 Volume 78(Issue 3) pp:809-813

Publication Date(Web):15 May 2009

DOI:10.1016/j.talanta.2008.12.054

It is found that Al(III) can further enhance the intensity of resonance light scattering (RLS) of the silver nanoparticles (AgNPs) and nucleic acids system. Based on this, a novel method of determination of nucleic acids is proposed in this paper. Under optimum conditions, there are linear relationships between the enhancing extent of RLS and the concentration of nucleic acids in the range of 1.0 × 10−9–1.0 × 10−7 g mL−1, 1.0 × 10−7–2.0 × 10−6 g mL−1 for fish sperm DNA (fsDNA), 1.0 × 10−9–7.0 × 10−8 g mL−1 for calf thymus DNA (ctDNA) and 1.0 × 10−9–1.0 × 10−7 g mL−1 for yeast RNA (yRNA). The detection limits (S/N = 3) of fsDNA, ctDNA and yRNA are 4.1 × 10−10 g mL−1, 4.0 × 10−10 g mL−1 and 4.5 × 10−10 g mL−1, respectively. The studies indicate that the RLS enhancement effect should be ascribed to the formation of AgNPs–Al(III)–DNA aggregations through electrostatic attraction and adsorption bridging action of Al(III). And the sensitivity and stability of the AgNPs–fsDNA system could be enhanced by Al(III).

Fluorescence enhancement effect of morin-nucleic acid-L-cysteine-capped nano-ZnS system and the determination of nucleic acid

Co-reporter:Liangliang Zhao, Xia Wu, Honghong Ding and Jinghe Yang

Analyst 2008 vol. 133(Issue 7) pp:896-902

Publication Date(Web):03 Apr 2008

DOI:10.1039/B800268A

It is found that L-cysteine-capped nano-ZnS can further enhance the fluorescence intensity of the morin–nucleic acid system. Under optimum conditions, the enhanced intensity of fluorescence is proportional to the concentration of nucleic acid in the range of 7.0 × 10−8–1.0 × 10−5 g mL−1 for fish sperm DNA (fsDNA) and 9.0 × 10−8–5.0 × 10−6 g mL−1 for yeast RNA (yRNA). The corresponding detection limits (S/N = 3) are 2.0 × 10−8 g mL−1 and 4.0 × 10−8 g mL−1, respectively. The interaction mechanisms of morin–nucleic acid–L-cysteine-capped nano-ZnS system are studied by multiple techniques. It is considered that there exists synergistic effects of groove binding and electrostatic interaction between morin, L-cysteine-capped nano-ZnS and nucleic acid, and the complex of morin–L-cysteine-capped nano-ZnS-nucleic acid is formed.

Resonance light-scattering enhancement effect of the proteinY3+TTASLS system and its analytical application

Co-reporter:Changying Guo;Wei Xu;Jinghe Yang

Luminescence 2008 Volume 23( Issue 6) pp:404-409

Publication Date(Web):

DOI:10.1002/bio.1053

Abstract

In this paper, a sensitive resonance light scattering (RLS) method for the determination of protein is reported. In the Tris–HCl (pH 7.50) buffer, protein enhanced the RLS intensity of the Y³⁺–2-thenoyltrifluoroacetone (TTA)–sodium dodecyl sulphate (SLS) system. The enhanced RLS intensities were in proportion to the concentrations of proteins in the range 8.0 × 10⁻⁹–1.0 × 10⁻⁵ g/mL for BSA, 1.0 × 10^–8–1.0 × 10⁻⁵ g/mL for HSA and 1.0 × 10^–8–1.0 × 10⁻⁶g/mL for EA, and their detection limits were 5.0, 5.4 and 6.7 ng/mL, respectively. Actual samples were satisfactorily determined. The interaction mechanism was also studied. Copyright © 2008 John Wiley & Sons, Ltd.

Study on the interaction between silver nanoparticles and nucleic acids in the presence of cetyltrimethylammonium bromide and its analytical application

Co-reporter:Jinhua Zheng, Xia Wu, Minqin Wang, Dehuan Ran, Wei Xu, Jinhe Yang

Talanta 2008 Volume 74(Issue 4) pp:526-532

Publication Date(Web):15 January 2008

DOI:10.1016/j.talanta.2007.06.014

A novel method is proposed in this paper, that is the silver nanoparticle (nanoAg)–cetyltrimethylammonium bromide (CTMAB) is used as the probe of resonance light scattering (RLS) for the determination of nucleic acids. Under optimum conditions, there are linear relationships between the quenching extent of RLS and the concentration of nucleic acids in the range of 4.0 × 10−9–2.0 × 10−6 g mL−1 for fish sperm DNA (fsDNA), 7.0 × 10−9–1.8 × 10−6 g mL−1 for calf thymus DNA (ctDNA) and 6.0 × 10−9–1.0 × 10−6 g mL−1 for yeast RNA (yRNA). The detection limits (S/N = 3) of fsDNA, ctDNA and yRNA are 2.7 × 10−10 g mL−1, 4.8 × 10−10 g mL−1 and 7.2 × 10−10 g mL−1, respectively. The studies indicate that there are interactions among nanoAg, CTMAB and fsDNA through electrostatic and chemical affinity, and the nanoAg–CTMAB complex can induce the structural change of base stacking and helicity of fsDNA.

Ag island film-enhanced rare earth co-luminescence effect of Tb–Gd–protein–sodium dodecyl benzene sulfonate system and sensitive detection of protein

Co-reporter:Chang Xia Sun, Xia Wu, Hai Ping Zhou, Fei Wang, Hong Hong Ding, Liang Liang Zhao, Jing He Yang

Talanta 2008 Volume 76(Issue 5) pp:1047-1052

Publication Date(Web):15 September 2008

DOI:10.1016/j.talanta.2008.05.001

Study on the interaction between oxolinic acid aggregates and protein and its analytical application

Co-reporter:Xia Wu, Jinhua Zheng, Honghong Ding, Dehuan Ran, Wei Xu, Yuanyuan Song, Jinghe Yang

Analytica Chimica Acta 2007 Volume 596(Issue 1) pp:16-22

Publication Date(Web):16 July 2007

DOI:10.1016/j.aca.2007.05.051

It was found that oxolinic acid (OA) at high concentration can self-assemble into nano- to micro- meter scale OA aggregates in Tris–HCl (pH 7.48) buffer solution. The nanoparticles of OA were adopted as fluorescence probes in the quantitative analysis of proteins. Under optimum conditions, the fluorescence quenching extent of nanometer scale OA aggregates was in proportion to the concentration of albumins in the range of 3.0 × 10−8 to 3.0 × 10−5 g mL−1 for bovine serum albumin (BSA) and 8.0 × 10−8 to 8.0 × 10−6 g mL−1 for human serum albumin (HSA). The detection limits (S/N = 3) were 3.4 × 10−9 g mL−1 for BSA, and 2.6 × 10−8 g mL−1 for HSA, respectively. Samples were satisfactorily determined. The interaction mechanism of the system was studied using fluorescence, UV–vis, resonance light scattering (RLS) and transmission electron microscope (TEM) technology, etc., indicating that the nonluminescent complex was formed between serum albumin molecular and OA, to disaggregate the self-association of OA, which resulted in the dominated static fluorescence quenching in the system.

Study on the Interaction between Florasulam and Bovine Serum Albumin

Co-reporter:Dehuan Ran;Jinhua Zheng;Jinghe Yang;Haiping Zhou

Journal of Fluorescence 2007 Volume 17( Issue 6) pp:721-726

Publication Date(Web):2007 November

DOI:10.1007/s10895-007-0226-9

In this paper, the interaction between florasulam (FU, 2′,6′,8-trifluoro-5-methoxy [Kragh-Hansen U, Molecular aspects of ligand binding to serum albumin. Pharmacol Rev 33(1):17–53 1981; Carter DC and Ho JX, Structure of serum albumin. Adv Protein Chem 45:153–203 1994; He XM, and Carter DC, Atomic structure and chemistry of human serum albumin. Nature 358(6383):209–215 1992] triazolo [1,5-c]pyrimidine-2-sulfonanilide) and bovine serum albumin (BSA) was investigated by fluorescence, ultraviolet absorption (UV) and Far-UV circular dichroism (CD) spectrometries. A strong fluorescence quenching was observed and the quenching mechanism was considered as static quenching. The binding constant of FU with BSA at 299 and 309 K were obtained as 1.5 × 104 and 7.1 × 103 l mol−1, respectively. There was one binding site between FU and BSA. The thermodynamic parameters enthalpy change (ΔH) and entropy change (ΔS) were calculated as −57.89 kJ mol−1 and −113.6 J mol−1 K−1, respectively, which indicated that the acting force between FU and BSA was mainly hydrogen bond and Van der Waals force. According to the Förster non-radiation energy transfer theory, the average binding distance between donor (BSA) and acceptor (FU) was obtained (r = 1.59 nm). The investigations of the UV/Vis and CD spectra of the system showed that the conformation of BSA was changed in presence of FU.

Determination of proteins using fluorescence enhancement of Tb3+–benzoylacetone–sodium dodecyl benzene sulfonate–protein system

Co-reporter:Changying Guo, Xia Wu, Jinghe Yang, Fei Wang, Zhen Jia, Dehuan Ran, Jinhua Zheng

Journal of Photochemistry and Photobiology A: Chemistry 2006 Volume 181(Issue 1) pp:50-55

Publication Date(Web):5 July 2006

DOI:10.1016/j.jphotochem.2005.10.032

A new system for the determination of proteins has been developed in this paper. Proteins can enhance the fluorescence intensity of the Tb–benzoylacetone (BA) system in the presence of sodium dodecyl benzene sulfonate (SDBS). Under optimum conditions, the enhanced fluorescence intensity is in proportion to the concentration of proteins in the range of 1.0 × 10−8 to 6.0 × 10−6 g mL−1 for bovine serum albumin (BSA), 3.0 × 10−8 to 6.0 × 10−6 g mL−1 for egg albumin (EA) and 1.0 × 10−8 to 5.0 × 10−6 g mL−1 for human serum albumin (HSA). Their detection limits (S/N = 3) are 3.9 × 10−9, 4.0 × 10−9 and 8.5 × 10−9 g mL−1, respectively. In addition, the interaction mechanism is also investigated.