•We proposed a new strategy based on electrospray technique to prepare dual-responsive nanogels of pH and redox sensitive.•A biodegradable and multi-responsive poly(ether urethane) (PEU) was synthesized via a facile one-pot method and used as the electrospray material.•The electrospray dual-responsive nanogels may have potential to be a tumor-sensitive drug delivery system.•It demonstrated that the materials which can form hydrogels in physiological condition may be used to prepare nanogels by electrospray method.•It implies that the application range of these materials may be expanded with the more simple and efficient electrospray method for preparing nanogels.In this work, we proposed a new strategy based on electrospray technique to prepare nanogels. Compared with other methods of preparing nanogels, electrospray technique is more simple and efficient. A biodegradable and multi-responsive poly(ether urethane) (PEU) was synthesized via a facile one-pot method and used as the electrospray material. By using electrospray technique, pH- and redox-responsive poly(ether urethane) nanogels were prepared. The morphologies of the electrospray nanoparticles before and after swelling were demonstrated to be spherical and uniform, as characterized by scanning electron microscope (SEM) and transmission electron microscopy (TEM). Dynamic light scattering (DLS) results showed that the mean hydrodynamic diameter of nanogels was about 500 nm. The pH- and redox-sensitive behaviors of nanogels were studied with DLS and TEM. In acidic media the nanogels dissociated, while in the presence of GSH the nanogels degraded. The nanogels suspension was stored at 4 °C and was stable without aggregation for at least 30 days. Doxorubicin (DOX) can be further loaded into the poly(ether urethane) nanogels. The electrospray nanogels can change the release rate of loaded drug in response to pH and GSH stimuli.