Yong Liang

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Name: 梁勇; Yong Liang

Organization: South China Normal University , China

Department: School of Chemistry and Environment

Title: Professor(PhD)

TOPICS

Analytical Chemistry

Chemicals
References

Preparation of core-shell magnetic molecular imprinted polymer with binary monomer for the fast and selective extraction of bisphenol A from milk

Co-reporter:Yanhao Yuan, Yan Liu, Weidi Teng, Jiean Tan, Yong Liang, Youwen Tang

Journal of Chromatography A 2016 Volume 1462() pp:2-7

Publication Date(Web):2 September 2016

DOI:10.1016/j.chroma.2016.06.045

•An enhanced imprinting method based on binary functional monomers was chosen for BPA imprinting.•The magnetic MIPs nano materials were synthesized by using surface-imprinted core-shell magnetic beads.•The magnetic MIPs showed better extraction capacity and good selectivity than that of NIPs for bisphenol A.•We were developed a method for the determination of bisphenol A in milk samples by magnetic MIPs extraction couple with HPLC.In the current study, a new strategy for the extraction of bisphenol A (BPA) from milk has been employed by using surface-imprinted core-shell magnetic beads, prepared by the reversible addition-fragmentation chain transfer (RAFT) polymerization. In order to obtain highly selective recognition cavities, an enhanced imprinting method based on binary functional monomers, e.g. 4-vinylpyridine (4-VP) and β-cyclodextrin (β-CD), was chosen for BPA imprinting. The morphological and magnetic properties of the Fe3O4-MIP beads were characterized by Transmission Electron Microscopy (TEM), Fourier Transform Infrared (FT-IR) Spectroscopy, Thermogravimetric Analysis (TGA), and Vibrating Sample Magnetometer (VSM). The characterization results suggested that MIP was synthesized evenly on Fe3O4-SiO2 surface. The adsorption experiments revealed that Fe3O4-MIPs showed better extraction capacity and selectivity toward BPA and its analogues than the non-imprinted polymers (NIPs). The saturation capacity of Fe3O4-MIP was 17.98 mg/g. In milk samples, the present method displayed a lower the detection thresholds, down to 3.7 μg/L. The recoveries of BPA in milk samples for three concentrations were found to be within 99.21%, 98.07% and 97.23%, respectively to three concentrations: 1.0 μmol/L, 10.0 mol/L, 100.0 μmol/L. Thus, the MIPs can be used for remove BPA in milk samples.

Simultaneous Analysis of Hydrochlorothiazide, Triamterene and Reserpine in Rat Plasma by HPLC and DSPE

Co-reporter:Xiaogang Chen;Weidi Teng;Lei Miao;Yan Wu;Dazhi Chen;Liyan Huang

Chromatographia 2016 Volume 79( Issue 7-8) pp:451-456

Publication Date(Web):2016 April

DOI:10.1007/s10337-016-3060-x

A dispersive solid-phase extraction (DSPE) method of mixing two different materials (C18 and MAX) was developed as extraction procedure, which provided better extraction yields (>82.1 %) for all analytes and more appropriate sample purification from endogenous interference materials compared with common SPE. Analyte separation was achieved on a C18 reversed phase column at the wavelength of 265 nm by high performance liquid chromatography (HPLC). The method was validated in terms of extraction yield, precision and accuracy. These assays gave mean accuracy values higher than 89 % with RSD values which were always less than 4.1 %. The method has a more simple operation process and achieves higher accuracy and precision than SPE. It has been successfully applied to plasma samples from rats after oral administration of target compounds.

Selective recognition of Triamterene in biological samples by molecularly imprinted monolithic column with a pseudo template employed

Co-reporter:Xiao-Yun Zhao;Hong-Wu Zhang;Zhen-Jie Liang;Ya-Ping Shu

Journal of Separation Science 2013 Volume 36( Issue 9-10) pp:1501-1508

Publication Date(Web):

DOI:10.1002/jssc.201201104

Melamine (MAM) was employed as a pseudo template to prepare a molecularly imprinted polymer monolithic column which presents the ability of selective recognition to Triamterene (TAT), whose structure was similar to that of MAM. Methacrylic acid and ethylene glycol dimethacrylate were applied as functional monomer and cross-linker, respectively, during the in situ polymerization process. Chromatographic behaviors were evaluated, the results indicated that the molecularly imprinted polymer monolithic column possessed excellent affinity and selectivity for TAT, and the imprinting factor was high up to 3.99 when 7:3 of ACN/water v/v was used as mobile phase. In addition, the dissociation constant and the binding sites were also determined by frontal chromatography as 134.31 μmol/L and 132.28 μmol/g, respectively, which demonstrated that the obtained molecularly imprinted polymer monolith had a high binding capacity and strong affinity ability to TAT. Furthermore, biological samples could be directly injected into the column and TAT was enriched with the optimized mobile phase. These assays gave recovery values higher than 91.60% with RSD values that were always less than 3.5%. The molecularly imprinted monolithic column greatly simplified experiment procedure and can be applied to preconcentration, purification, and analysis of TAT in biological samples.

Electrochemical detection of dopamine in the presence of ascorbic acid using PVP/graphene modified electrodes

Co-reporter:Qin Liu, Xu Zhu, Zhaohui Huo, Xulun He, Yong Liang, Maotian Xu

Talanta 2012 Volume 97() pp:557-562

Publication Date(Web):15 August 2012

DOI:10.1016/j.talanta.2012.05.013

Graphene (GR) was synthesized through electrochemical reduction of graphene oxide and characterized by spectroscopic and electrochemical techniques. Polyvinylpyrrolidone (PVP)/graphene modified glassy carbon electrode (PVP/GR/GCE) was prepared and applied for the fabrication of dopamine (DA) sensors without the interference of ascorbic acid (AA). Compared to bare GCE, an increase of current signal was observed, demonstrating that PVP/GR/GCE exhibited favorable electron transfer kinetics and electrocatalytic activity towards the oxidation of dopamine. Furthermore, PVP/GR/GCE exhibited good ability to suppress the background current from large excess ascorbic acid. Amperometric response results show that the PVP based sensor displayed a wide linear range of 5×10−10 to 1.13×10−3 mol/L DA with a correlation coefficient of 0.9990 and a detection limit of 0.2 nM (S/N=3). The determination of dopamine in urine and human serum samples were studied.Highlights► PVP/GR/GCE showed good sensitivity, selectivity and reproducibility towards the detection of DA. ► The modified electrode can eliminate the interference of AA, UA and some co-existing substances. ► The modified electrode can determine DA in urine and human serum samples without pretreatment.

Simultaneous analysis of hydrochlorothiazide, triamterene and reserpine in rat plasma by high performance liquid chromatography and tandem solid-phase extraction

Co-reporter:Hang Li;Junting He;Qin Liu;Zhaohui Huo;Si Liang

Journal of Separation Science 2011 Volume 34( Issue 5) pp:542-547

Publication Date(Web):

DOI:10.1002/jssc.201000754

Abstract

A tandem solid-phase extraction method (SPE) of connecting two different cartridges (C₁₈ and MCX) in series was developed as the extraction procedure in this article, which provided better extraction yields (>86%) for all analytes and more appropriate sample purification from endogenous interference materials compared with a single cartridge. Analyte separation was achieved on a C₁₈ reversed-phase column at the wavelength of 265 nm by high-performance liquid chromatography (HPLC). The method was validated in terms of extraction yield, precision and accuracy. These assays gave mean accuracy values higher than 89% with RSD values that were always less than 3.8%. The method has been successfully applied to plasma samples from rats after oral administration of target compounds.

Sensitive simultaneous determination of catechol and hydroquinone using a gold electrode modified with carbon nanofibers and gold nanoparticles

Co-reporter:Zhaohui Huo;Yanli Zhou;Qin Liu;Xulun He;Maotian Xu

Microchimica Acta 2011 Volume 173( Issue 1-2) pp:119-125

Publication Date(Web):2011 April

DOI:10.1007/s00604-010-0530-y

A highly sensitive electrochemical sensor for the simultaneous determination of catechol (CC) and hydroquinone (HQ) was fabricated by electrodeposition of gold nanoparticles onto carbon nanofiber film pre-cast on an Au electrode. Both CC and HQ cause a pair of quasi-reversible and well-defined redox peaks at the modified electrode in pH 7.0 solution. Simultaneously, the oxidation peak potentials of CC and HQ become separated by 112 mV. When simultaneously changing the concentrations of both CC and HQ, the response is linear between 9.0 μM and 1.50 mM. In the presence of 0.15 mM of the respective isomer, the electrode gives a linear response in the range from 5.0 to 350 μM, and from 9.0 to 500 μM for CC and HQ, respectively, and detection limits are 0.36 and 0.86 μM. The method was successfully examined for real sample analysis with high selectivity and sensitivity.

Application of high-speed counter-current chromatography and preparative high-performance liquid chromatography mode for rapid isolation of anthraquinones from Morinda officinalis How.

Co-reporter:Licai Zhu, He Li, Yong Liang, Xiaohong Wang, Huichun Xie, Tianyou Zhang, Yoichiro Ito

Separation and Purification Technology 2009 Volume 70(Issue 2) pp:147-152

Publication Date(Web):10 December 2009

DOI:10.1016/j.seppur.2009.09.009

Five anthraquinones including alizarin-1-methylether, 1,2-dimethoxy-3-hydroxyanthraquinone, 1-hydroxy-3-hydroxymethylanthraquinone, rubiadin-1-methylether and anthragallol-2-methylether were isolated and purified by high-speed counter-current chromatography (HSCCC) and followed by preparative high-performance liquid chromatography (prep-HPLC) mode from Morinda officinalis How. n-Hexane–ethyl acetate–methanol–water (6:4:5:5, v/v/v/v) was employed as the two-phase solvent system in HSCCC. Consequently, partially purified fraction “1” (90.6 mg, containing alizarin-1-methylether and 1,2-dimethoxy-3-hydroxyanthraquinone), partially purified fraction “2” (52.5 mg, containing 1-hydroxy-3-hydroxymethylanthraquinone and rubiadin-1-methylether) and 19.8 mg anthragallol-2-methylether were obtained from 300 mg of the crude extract. Then the partially purified fractions were further separated by prep-HPLC, which recovered 54.9 mg alizarin-1-methylether, 10.2 mg 1,2-dimethoxy-3-hydroxyanthraquinone, 16.4 mg 1-hydroxy-3-hydroxymethylanthraquinone and 18.2 mg rubiadin-1-methylether. The purity of each compound was over 96%, as determined by HPLC. The structures of five anthraquinones were identified by MS and 1H NMR. It is the first report of discovering 1,2-dimethoxy-3-hydroxyanthraquinone from the plant of M. officinalis How. The results demonstrated that this separation mode can integrate the advantages of HSCCC and prep-HPLC to improve separation efficiency, and adopted method was a feasible, economical and efficient technique for rapid preparative isolation of complicated natural products.