ZhengJian Qi

A simple salicylaldehyde-based fluorescent probe 1 for Zn2+ was prepared. The probe 1 has been proven to be highly selective and very sensitive to Zn2+, and also shows remarkable fluorescence emission improvement upon combining with Zn2+ in a Tris–HCl buffer system at pH 7.2. The great fluorescence changes of probe 1 after incorporation with Zn2+ are mainly ascribed to the photo-induced electron transfer (PET) mechanism. The experimental results reveal that probe 1 could effectively complex Zn2+ at a molar ratio of about 1 : 1 and the binding constant (K) between probe 1 and Zn2+ was determined to be 3.78 × 106 M−1. The detection limit (DL) of probe 1 was also calculated to be 5.9 × 10−9 M. In addition, the probe has been successfully applied to fluorescence imaging in living MCF-7 cells.

A novel cyclometalated Ir(III) complex based luminescence intensity and lifetime sensor for Cu2+

Co-reporter:Wei Shen, Zhengjian Qi, Liqiang Yan, Wenwen Tian, Xia Cui, Hongtao Yao and Yueming Sun

RSC Advances 2016 vol. 6(Issue 20) pp:16482-16488

Publication Date(Web):28 Jan 2016

DOI:10.1039/C5RA27189D

We report the synthesis and characterization of two luminescent cyclometalated Ir(III) complexes [Ir(dfppy)2(bpy-DPA)]PF6 (Ir-1) and [Ir(dfppy)2(bpy-BiDPA)]PF6 (Ir-2), where dfppy, bpy-DPA and bpy-BiDPA represent 2-(2,4-difluorophenyl)pyridine, N-([2,2′-bipyridine]-4-yl)-2-(bis(pyridin-2-ylmethyl)amino)acetamide and N,N′-([2,2′-bipyridine]-4,4′-diyl)bis(2-(bis(pyridin-2-ylmethyl)amino)acetamide), respectively. Their photophysical properties and sensing properties towards various metal ions were investigated at room temperature. The two complexes both possessed good photophysical properties, and Ir-2 had quite a high quantum yield (93.83%) and a long lifetime (101.17 μs), which is impressive among Ir(III) complexes. Ir-2 exhibited both high selectivity and sensitivity towards Cu2+ over other metal ions. The Job curve and mass spectra suggested the formation of a 1:2 bonding mode between Ir-2 and Cu2+. The photoluminescence (PL) intensity quenching curve indicated that Ir-2 could enable rapid and reversible detection of Cu2+ with a low detection limit of 0.85 ppb (13 nM). Furthermore, a linear relationship could be observed between the PL lifetime value of Ir-2 and the concentration of Cu2+ ions in the range of 0–8 μM. These results suggested that Ir-2 might have use as not only a promising photoluminescence intensity sensor, but also a promising lifetime sensor for Cu2+ in aqueous solution.

Synthesis and optical properties of aggregation-induced emission (AIE) molecules based on the ESIPT mechanism as pH- and Zn2+-responsive fluorescent sensors

Co-reporter:Liqiang Yan, Tingting Qing, Renjie Li, Zhongwei Wang and Zhengjian Qi

RSC Advances 2016 vol. 6(Issue 68) pp:63874-63879

Publication Date(Web):29 Jun 2016

DOI:10.1039/C6RA09920C

A series of schiff base derivatives with salicylaldehyde moieties were designed and synthesized. These materials exhibit typical AIE properties and high fluorescence quantum yields in an aggregated state based on the excited-state intramolecular proton transfer (ESIPT) mechanism. Then, the effect of substituted group types and positions on the fluorescence of these novel compounds was investigated through fluorescence spectra analysis and theoretical calculations. And it was found that the salicylaldehyde derivatives can be used as potential pH and Zn2+ sensors.

A novel coumarin-based red fluorogen with AIE, self-assembly, and TADF properties

Co-reporter:Li Qiang Yan, Zhi Neng Kong, Yong Xia and Zheng Jian Qi

New Journal of Chemistry 2016 vol. 40(Issue 8) pp:7061-7067

Publication Date(Web):14 Jun 2016

DOI:10.1039/C6NJ01296E

Luminescent materials with aggregation-induced emission (AIE) properties have attracted considerable interest for their promising applications in light-emitting and display devices and fluorescent chemosensors. Coumarin and its derivatives are one of the most attractive chromophores due to their excellent properties, such as high fluorescence quantum yield, large Stokes shift, less toxicity, ease of modification and visible wavelength emission. A coumarin-based AIE-active red fluorogen was designed and synthesised. It was found that CDPA exhibits AIE property, red emission, self-assembly and thermally activated delayed fluorescence (TADF) in the solid state.

An aggregation-induced emission (AIE) ratiometric fluorescent cysteine probe with an exceptionally large blue shift

Co-reporter:Liqiang Yan, Zhineng Kong, Wei Shen, Wenqi Du, Yan Zhou and Zhengjian Qi

RSC Advances 2016 vol. 6(Issue 7) pp:5636-5640

Publication Date(Web):07 Jan 2016

DOI:10.1039/C5RA22245A

In this work, we developed a novel colorimetric and ratiometric fluorescence probe for the selective and sensitive monitoring of cysteine based on a coumarin Schiff-base platform. This probe exhibited remarkable AIE characteristics with an exceptionally large blue shift (125 nm), which showed low emission in DMSO but strong aggregation-induced emission in water solution. An excellent linear relationship of fluorescence intensity ratio (F450/F575) (R2 = 0.9922) versus Cys concentration in the range of 1–13 μM was obtained.

A novel aggregation induced emission active cyclometalated Ir(III) complex as a luminescent probe for detection of copper(II) ion in aqueous solution

Co-reporter:Wei Shen, Liqiang Yan, Wenwen Tian, Xia Cui, Zhengjian Qi, Yueming Sun

Journal of Luminescence 2016 Volume 177() pp:299-305

Publication Date(Web):September 2016

DOI:10.1016/j.jlumin.2016.04.048

We report the synthesis and characterization of a novel aggregation induced emission (AIE) active cyclometalated Ir(III) complex, namely [Ir(dfppy)2(phen-DPA)]PF6, where dfppy and phen-DPA represent 2-(2,4-difluorophenyl)pyridine and 2-(bis(pyridin-2-ylmethyl)amino)-N-(1,10-phenanthrolin-5-yl)acetamide, respectively. The complex showed remarkable selectivity for copper(II) in aqueous solution over other competitive ions. Furthermore, this sensor showed a rapid and reversible response to copper(II) in aqueous solution with a detection limit of 65 nM.

A label-free turn-on fluorescence probe for rapidly distinguishing cysteine over glutathione in water solution

Co-reporter:Liqiang Yan, Zhineng Kong, Wei Shen, Wenqi Du, Yan Zhou, Zhengjian Qi

Analytical Biochemistry 2016 500() pp: 1-5

Publication Date(Web):1 May 2016

DOI:10.1016/j.ab.2016.01.027

A novel label-free fluorescent chemodosimeter (C1) was synthesized, based on coumarin and N-(4-aminobenzoyl)-β-alanine, for the selective detection of cysteine (Cys) over glutathione (GSH), which involved a click reaction of Cys to CN of a Schiff base. The probe C1 featured a fast response (about 3 min), emission in the visible region, and high selectivity. Addition of Cys in HEPES-NaOH solution (pH 7.4) to C1 in water resulted in the appearance of a new emission peak at 445 nm, in company with remarkable enhancement of fluorescence intensity, while other amino acids did not induce any significant fluorescence change. Meanwhile, the addition reaction of Cys to C1 elicited 90.8-fold fluorescence intensity enhancement, which resulted in a change of emission color from orange to blue.

Cationic ring opening polymerization of octamethylcyclotetrasiloxane initiated by solid superacid

Co-reporter:Yong Xia;Du Cao;Yu Sun;Fengfu Li

Glass Physics and Chemistry 2016 Volume 42( Issue 3) pp:307-311

Publication Date(Web):2016 May

DOI:10.1134/S1087659616030184

Kinetics of the cationic ring opening polymerization of octamethylcyclotetrasiloxane (D4) in bulk initiated by solid superacid was studied. The optimalizing experimental conditions were based on preliminary experiments. Higher temperature was beneficial to equilibrium conversion while stirring intensity beyond a certain level displayed no obvious effect on the rate of the reaction. A kinetic model was elicited and kinetic parameters were obtained through optimization. The calculated activation energies were 32.6 and 34.1 kJ mol–1 for D4 and all other annuluses (Dx), respectively.

A new turn on coumarin-based fluorescence probe for Ga3 + detection in aqueous solution

Co-reporter:Liqiang Yan, Yan Zhou, Wenqi Du, Zhineng Kong, Zhengjian Qi

Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy 2016 Volume 155() pp:116-124

Publication Date(Web):15 February 2016

DOI:10.1016/j.saa.2015.11.012

•A new fluorescent probe based on coumarin containing l-threonine was designed and synthesized succinctly.•The probe has been proven to be high selective and sensitive for the analysis of Ga3 + in pure water solution.•The probe could respond to Ga3 + in a short time.•The association constant between probe and Ga3 + was found to be 3.7 × 104 M− 1.The probe CT was synthesized and investigated as a novel label-free chemosensor for Ga3 + detection in water. Probe CT showed remarkable selectivity and sensitivity for Ga3 + in Tris–HCl aqueous buffer solution (pH 7.0). The chemosensor responded rapidly to Ga3 + with a 1:1 stoichiometry. Meanwhile, the unapparent changes of fluorescence lifetime decays suggest the turn-on process of probe CT by Ga3 + which appears to be a static mechanism.

Self-assembly oxygen sensing thin film based on a Ru(II) complex covalently grafted to porous anodized alumina oxide (AAO) templates

Co-reporter:Meifang Cui, Fang Wang, Zhihui Miao, Yong Xia, Liqiang Yan, Yun Ma, Hongtao Yao, Huanhuan Ling and Zhengjian Qi

RSC Advances 2015 vol. 5(Issue 80) pp:65627-65634

Publication Date(Web):10 Jul 2015

DOI:10.1039/C5RA07809A

A novel functional Ru(bpy)2(phen-NH2)(PF6)2 complex (bpy = bipyridyl, phen-NH2 = 5-amine-1,10-phenanthroline) is covalently bonded to an anodized alumina oxide (AAO) template through a “soft bridge” of 3-glycidoxypropyltrimethoxysilane (GPMS). This can form an oxygen sensor with the structure [Ru(bpy)2(phen-NH)/GPMS/AAO]. This oxygen sensing thin film was characterized using scanning electron microscopy (SEM) and fluorescence spectrophotometry. A series of cluster structures were observed on the surface of the AAO, the Ru(bpy)2(phen-NH2) fluorophore which was uniformly dispersed. The film proved to have good oxygen quenching properties owing to the great interaction between the fluorophore and molecular oxygen. Finally, a Stern–Volmer plot of the [Ru(bpy)2(phen-NH)/GPMS/AAO] thin film shows a good linearity with a fitting parameter R2 of 0.9948 when the oxygen concentration is 0%–80%, and the sensitivity rises to 10.47, which is 7.5 times greater than that of oxygen sensors based on sol–gel methods. A short response/recovery time of 7 s/15 s was achieved, which is almost 100% faster than that of sol–gel methods with the same fluorophore. When stored in the ambient air of the laboratory, there was no significant drift in intensity after 6 months. These characteristics result in the thin film of [Ru(bpy)2(phen-NH)/GPMS/AAO] being a promising candidate for sensitive oxygen sensors.

Theoretical and experimental investigations on mono-substituted and multi-substituted functional polyhedral oligomeric silsesquioxanes

Co-reporter:Yong Xia, Hongtao Yao, Meifang Cui, Yun Ma, Zhineng Kong, Baoying Wu, Zhengjian Qi and Yueming Sun

RSC Advances 2015 vol. 5(Issue 98) pp:80339-80345

Publication Date(Web):16 Sep 2015

DOI:10.1039/C5RA14298A

The structure and electronic properties of polyhedral oligomeric silsesquioxane (POSS) cages functionalized with mono-substituted and multi-substituted vinyl and mercaptopropyl have been studied using density functional theory calculations. Electron density distributions and frontier molecular orbitals (FMOs) have been constructed at the B3LYP/6-31+G(d) level to understand the electronic properties. 1H, 13C, and 29Si NMR spectra by using the gauge including atomic orbital (GIAO) method of the studied compounds were compared with experimental data obtained.

Facile synthesis and self-assembly of amphiphilic polydimethylsiloxane with poly(ethylene glycol) moieties via thiol-ene click reaction

Co-reporter:Yong Xia, Hongtao Yao, Zhihui Miao, Yun Ma, Meifang Cui, Liqiang Yan, Huanhuan Ling and Zhengjian Qi

RSC Advances 2015 vol. 5(Issue 63) pp:50955-50961

Publication Date(Web):05 Jun 2015

DOI:10.1039/C5RA10150F

We demonstrate here a facile and efficient synthesis of polysiloxane-based amphiphilic copolymers via thiol-ene click chemistry. The properties of PEG-b-PDMS-b-PEG (T-PPG) and PDMS-g-PEG (S-PPG20 and S-PPG100) amphiphilic copolymers were studied in detail by a combination of 1H NMR, FT-IR, gel permeation chromatography, and thermogravimetric analysis. In comparison with the traditional hydrosilylation method which requires the noble metal catalyst platinum, the newly designed thiol-ene protocol produces polysiloxane-based amphiphilic copolymers with only anti-Markovnikov addition products under benign conditions. The resulting copolymers have lower critical aggregation concentration (CAC), and dynamic light scattering (DLS) results revealed that the obtained amphiphilic copolymers can self-assemble into nanoparticles (128 nm to 198 nm) in aqueous solutions with a narrow size distribution (PDI is less than 0.24).

A novel coumarin-based fluorescence chemosensor containing L-histidine for aluminium(III) ions in aqueous solution

Co-reporter:Li-Qiang Yan, Yun Ma, Mei-Fang Cui and Zheng-Jian Qi

Analytical Methods 2015 vol. 7(Issue 15) pp:6133-6138

Publication Date(Web):18 Jun 2015

DOI:10.1039/C5AY01466B

A coumarin-based “Turn-on” fluorescence chemosensor 1 containing L-histidine for Al3+ was readily synthesized and investigated, which showed high selectivity and remarkable sensitivity to aluminium(III) ions based on a photo-induced electron-transfer (PET) mechanism in aqueous solution (Tris–HCl, 0.1 mM, pH 7.2). The sensor responded rapidly to Al3+ in aqueous solutions with a 1:1 stoichiometry. Meanwhile, it showed excellent adaptability and also responsiveness.

Sensitive and fluorescence “turn-on” detection of BRCA1 and TB4 DNA sequences using water-soluble conjugated polythiophenes

Co-reporter:Yun Ma, Yong Xia, Liqiang Yan, Fang Wang, Zhihui Miao, Meifang Cui, Hongtao Yao and Zhengjian Qi

Analytical Methods 2015 vol. 7(Issue 14) pp:5814-5819

Publication Date(Web):01 Jun 2015

DOI:10.1039/C5AY01127B

A biocompatible, label-free and sensitive fluorescence “turn-on” approach was designed to detect BRCA1 and TB4 DNA sequences using poly(3-[(S)-5-amino-5-carboxyl-3-oxapentyl]-2,5-thiophenylene hydrochloride) (POWT). The fluorescence intensity of POWT in solution could be efficiently quenched by adding either BRCA1 or TB4 DNA sequences. The Stern–Volmer equation was used to fit the quenching effect and the LOD could thus be obtained. The fluorescence would recover its initial intensity after the addition of completely complementary ssDNA, but not with sequences with 1–3 mismatched bases. The influences of buffer pH and buffer composition were investigated systematically in order to improve the sensitivity of this fluorescent probe. For the BRCA1 DNA sequence, the KSV (Stern–Volmer constant) and LOD were 8.254 × 109 M−1 and 2.231 × 10−12 M, respectively. For the TB4 DNA sequence, the KSV and LOD were 6.924 × 109 M−1 and 2.659 × 10−12 M, respectively. In addition, the quantitative linearity range for detecting these two DNA sequences was from 1 × 10−11 M to 5 × 10−11 M, and the response time for the quenching effect was less than 10 minutes for both sequences. Hence, with fine biocompatibility, high sensitivity, fast response and high recovery rate, a serine functionalized POWT might be a promising candidate for detecting the BRCA1 DNA sequence and TB4 DNA sequence as well as their mutations, which might possibly be applied to clinical diagnosis of breast cancer or ovarian tumours.

Theoretical investigation on electronic structures and stability of the dinuclear palladium and platinum complexes

Co-reporter:Yong Xia, Zhihui Miao, Fang Wang, Hongtao Yao, Meifang Cui, Yun Ma, Zhengjian Qi, Yueming Sun

Journal of Organometallic Chemistry 2015 Volume 779() pp:81-85

Publication Date(Web):1 March 2015

DOI:10.1016/j.jorganchem.2014.12.024

•The DFT computational study of the dinuclear palladium(0) and platinum(0) complexes.•There is no metal–metal σ-bond in the dinuclear complexes.•The electrostatic interaction dominates the metal–metal interaction.•The four dinuclear complexes are stable species.The equilibrium geometries, metal–metal interaction, and metal–ligand interaction of dinuclear palladium(0) and platinum(0) complexes (M2(bq)2(nbe)2 and M2(bq)2(dafo), where M is Pt and Pd, dafo is 4,5-diazafluoren-9-one) were investigated using density functional theory. Natural bonding orbital (NBO) analysis indicates that there is no metal–metal σ-bond and the electrostatic interaction dominates the metal–metal interaction. The values of second-order interaction energies (E2) confirm the strong metal–ligand interactions and stabilization effect of the metal atoms. Thermochemistry suggests all of the four complexes to be stable species. The class of potential complexes is predicted stable enough for practical applications as highly efficient catalyst.The DFT computational study of the electronic structures and stability of the dinuclear palladium(0) and platinum(0) complexes, M2(bq)2(nbe)2 and M2(bq)2(dafo), were reported using B3LYP method at 6-31+G(d) level.

An efficient electroluminescence copolymer based on vinyl-linked donoracceptor system

Co-reporter:Zheng-Jian Qi;Bin Wei;Yue-Ming Sun;Man-Xin Hong;Lan-Lan Tang;Shou-Gen Yin

Journal of Applied Polymer Science 2011 Volume 122( Issue 1) pp:50-54

Publication Date(Web):

DOI:10.1002/app.33694

Abstract

Using copolymerization functionalization, poly[3,4-dioctyloxythiophene-alt-2,5-bis(4-vinylphenyl)-1,3,4-oxadiazole] (P3,4DOOTV-OXD) combining electrochemical properties of poly(3,4-dioctyloxythiophene) (P3,4DOOT, donor) and 1,3,4-oxadiazole (OXD, acceptor) was synthesized through Heck reaction and evaluated with ¹H-NMR, FTIR, gel permeation chromatography (GPC), UV–vis spectroscopy, photoluminescence (PL), and electroluminescence (EL) spectra. This D/A alternating copolymer, with regiosymmetrical 3,4-dioctyloxythiophene, has good solubility in common organic solvents. The optical properties investigations showed that the band-gap energy of P3,4DOOTV-OXD is 10% higher than that of P3,4DOOT (2.27 eV) at 2.52 eV in solid film. However, the photoluminescence (PL) quantum yield is as high as 57.4%, > two times more than that of homopolymer P3,4DOOT in CHCl₃ solution, and displays bright green-yellow luminescence (518 nm) in solution and yellow (542 nm) in solid film. Cyclic voltammogram indicated that the copolymer has bipolar properties, and the band gap energy (E_g) calculated based on the cyclic voltammogram is consistent with that deduced from the absorption spectra at 2.40 eV. By using indium-tin oxide (ITO) as the anode, poly(ethylenedioxythiophene) (PEDOT) doped with poly(styrenesulfonate) (PSS) as the hole-injection layer, P3,4DOOTV-OXD as the emitter and aluminum (Al) as the cathode, double-layer device with the structure of [ITO/PEDOT: PSS (50 nm)/polymer/Al (130 nm)] shows typical diode characters and exhibit bright yellow electroluminescence (562–552 nm) under the forward bias. © 2011 Wiley Periodicals, Inc. J Appl Polym Sci, 2011

Optical and electronic properties of 3,4-dialkylthiophene-based p-/n-alternating copolymers

Co-reporter:Zheng-Jian Qi;Xue-Mei Wang;Bin Wei;Feng Kang;Lan-Lan Tang;Man-Xin Hong;Yue-Ming Sun

Journal of Applied Polymer Science 2011 Volume 120( Issue 5) pp:2678-2684

Publication Date(Web):

DOI:10.1002/app.33407

Abstract

Four novel highly soluble p-/n-poly[(2,5-divinyl-3,4-dialkylthiophene)-alt-2,6-pyridine] (PA₂TV-Py) and poly[(2,5-divinyl-3,4-dialkylthiophene)-alt-(2,5-diphenyl-1,3,4-oxadiazole)] (PA₂TV-OXD) are prepared by Heck coupling approach to compare their photoelectric properties. Characterizations of the copolymers include FT-IR, ¹H-NMR, gel permeation chromatography (GPC), thermogravimetric analysis (TGA), UV–vis spectroscopy, photoluminescence (PL), and electroluminescence (EL). Four alt-copolymers exhibit excellent solubility in common organic solvents (e.g., CHCl₃, THF) and good thermal stabilities, losing less than 5% on heating to ∼ 250°C. The optical properties depict that the band-gap energy of PA₂TV-Py and PA₂TV-OXD is similarly, ranging from 2.68 to 2.80 eV in solid film and 2.90–2.97 eV in CHCl₃ solution. PA₂TV-Pys can emit bright turquoise light with quantum efficiencies (QE) of 30.6 and 53.9%, which about 10-18 times higher than that of homopolymer in CHCl₃ solution. Furthermore, the QE of two PA₂TV-OXDs (purple fluorescence) are increased to 43.6 and 68.5%, respectively, about 1.3–1.4 times higher than that of PA₂TV-Pys. Electrochemical results indicate that the electron affinity (E_a) of four alt-copolymers range from 2.79 to 3.09 eV, which are propitious to electrons injecting and transporting from the cathode. As a result, these novel copolymers present expected good electroluminescence(EL) performance in their single layer polymer light-emitting device (PLED) with configuration of ITO/polymer/Al, which turn-on voltages are between 4.0 and 5.8 V and emit bright green–yellow (538 nm) and yellow (545–552 nm) EL light. © 2011 Wiley Periodicals, Inc. J Appl Polym Sci, 2011

Comparative study of photoelectric properties of regiosymmetrical poly(3,4-dialkoxythiophene)s

Co-reporter:Zheng Jian Qi;Bin Wei;Yue Ming Sun;Xue Mei Wang;Feng Kang

Polymer Bulletin 2011 Volume 66( Issue 7) pp:905-915

Publication Date(Web):2011 April

DOI:10.1007/s00289-010-0324-8

A series of regiosymmetrical poly(3,4-dialkoxythiophene)s: poly(3,4-diamyloxythiophene), poly(3,4-dioctyloxythiophene), poly(3,4-di(2-ethyl-1-hexyloxy)thiophene), and poly(3,4-didodecyloxythiophene) were synthesized by the FeCl3-oxidative approach. All these polymers were evaluated with NMR, FT-IR, gel permeation chromatography (GPC), thermo-gravimetric analysis (TGA), UV–Vis spectroscopy, and photoluminescence (PL). The polymers have excellent solubility in common organic solvents, and TGA studies show that the polymers lost 5% of their weights on heating to 250 °C above. Investigations of the UV–Vis spectroscopy show that the absorption maxima of the polymer thin films are similar to those in solutions, and the optical band gaps of the polymer thin films are ranging from 2.27 to 2.69 eV. In PL spectra, maxima emission peaks of the polymer thin films lie at 527 to 589 nm, embodying colors from green to yellow, and the quantum yields of the polymers are in the range of 22–28%. All the data indicate that the polymers have good solubility, outstanding thermal stabilities, low band gaps, and high PL quantum yields, and they might be excellent polymeric materials for applications in organic light-emitting diodes (OLEDs), light-emitting electrochemical cells, polymer solar cells, and so on.

A novel coumarin-based fluorescence chemosensor containing L-histidine for aluminium(III) ions in aqueous solution

Co-reporter:

Analytical Methods (2009-Present) 2015 - vol. 7(Issue 15) pp:NaN6138-6138

Publication Date(Web):2015/06/18

DOI:10.1039/C5AY01466B

Sensitive and fluorescence “turn-on” detection of BRCA1 and TB4 DNA sequences using water-soluble conjugated polythiophenes

Co-reporter:

Analytical Methods (2009-Present) 2015 - vol. 7(Issue 14) pp:NaN5819-5819

Publication Date(Web):2015/06/01

DOI:10.1039/C5AY01127B