Co-reporter:Hongyan Liu, Ju Cheng, Fengjuan Chen, Decheng Bai, Changwei Shao, Jun Wang, Pinxian Xi and Zhengzhi Zeng
Nanoscale 2014 vol. 6(Issue 10) pp:5315-5322
Publication Date(Web):20 Feb 2014
DOI:10.1039/C4NR00355A
We report a facile modification of graphene oxide (GO) by gelatin to mimic charged proteins present in the extracellular matrix during bone formation. The bioinspired surface of GO–gelatin (GO–Gel) composite was used for biomimetic mineralization of hydroxyapatite (HA). A detailed structural and morphological characterization of the mineralized composite was performed. Additionally, MC3T3-E1 cells were cultured on the GO–Gel surfaces to observe various cellular activities and HA mineralization. Higher cellular activities such as cell adhesion, cell proliferation, and alkaline phosphatase activity (ALP) were observed on the GO–Gel surface compared with the GO or glass surface. The increase of ALP confirms that the proposed GO–Gel promotes the osteogenic differentiation of MC3T3-E1 cells. Moreover, the evidence of mineralization evaluated by scanning electron microscopy (SEM) and alizarin red staining (ARS) corroborate the idea that a native osteoid matrix is ultimately deposited. All these data suggest that the GO–Gel hybrids will have great potential as osteogenesis promoting scaffolds for successful application in bone surgery.
Co-reporter:Hongyan Liu, Ju Cheng, Fengjuan Chen, Fengping Hou, Decheng Bai, Pinxian Xi, and Zhengzhi Zeng
ACS Applied Materials & Interfaces 2014 Volume 6(Issue 5) pp:3132
Publication Date(Web):February 14, 2014
DOI:10.1021/am4057826
In bone tissue engineering, it is imperative to design multifunctional biomaterials that can induce and assemble bonelike apatite that is close to natural bone. In this study, graphene oxide (GO) was functionalized by carrageenan. The resulting GO-carrageenan (GO-Car) composite was further used as a substrate for biomimetic and cell-mediated mineralization of hydroxyapatite (HA). It was confirmed that carrageenan on the GO surface facilitated the nucleation of HA. The observation of the effect of the GO-Car on the adhesion, morphology, and proliferation of MC3T3-E1 cells was investigated. In vitro studies clearly show the effectiveness of GO-Car in promoting HA mineralization and cell differentiation. The results of this study suggested that the GO-Car hybrid will be a promising material for bone regeneration and implantation.Keywords: Biomimetic mineralization; carrageenan; cell-mediated; graphene oxide; hydroxyapatite;
Co-reporter:Fengjuan Chen, Guozhen Liu, Yanjun Shi, Pinxian Xi, Ju Cheng, Jinpeng Hong, Rong Shen, Xiaojun Yao, Decheng Bai, Zhengzhi Zeng
Talanta 2014 Volume 124() pp:139-145
Publication Date(Web):15 June 2014
DOI:10.1016/j.talanta.2014.02.034
•The novel coumarin-derived chemosensor L is simple to synthesize.•The structure of the chemosensor L was confirmed by single crystal X-ray diffraction.•The chemosensor L can be used to determine Cu2+ ion with high selectivity can be used for imaging the Cu2+ ion in cells.•The fluorescence turn on–off mechanism and coordination mode were studied by DFT calculations.A novel coumarin-based fluorescent probe L ((4E)-4-((7-hydroxy-4-methyl-2-oxo-2H-chromen-8-yl) methyleneamino)-1,2-dihyydro-2,3-dimethyl-1-phenylpyrazol-5-one) has been developed as a simple and efficient chemosensor which exhibits a significant fluorescence reduction in the presence of metal cations. This sensor exhibits high selectivity and sensitivity toward Cu2+ over other common cations. The mechanism for detecting copper was evaluated by time-dependent density functional theory (TD-DFT) calculations and the coordination mode was also confirmed by density functional theory (DFT) calculations. Furthermore, results of cell imaging in this study indicate that this new probe may be useful for detection and monitoring of Cu2+ in biological applications.Chemosensor L was reported as a fluorescence probe for Cu2+, it exhibited high selectivity and sensitivity toward Cu2+ over other co-existed cations.
Co-reporter:Pinxian Xi, Fengjuan Chen, Guoqiang Xie, Cai Ma, Hongyan Liu, Changwei Shao, Jun Wang, Zhihong Xu, Ximing Xu and Zhengzhi Zeng
Nanoscale 2012 vol. 4(Issue 18) pp:5597-5601
Publication Date(Web):31 May 2012
DOI:10.1039/C2NR31010D
In this study, monodisperse palladium (Pd) nanoparticles on reduced graphene oxide (RGO) surfaces were successfully prepared by a “wet” and “clean” method in aqueous solution. Without any surface treatment, Pd nanoparticles are firmly attached to the RGO sheets. These RGO/Pd nanocomposites exhibited catalytic activity in hydrogen generation from the hydrolysis of ammonia borane (AB). Their hydrolysis completion time and activation energy were 12.5 min and 51 ± 1 kJ mol−1, respectively, which were comparable to the best Pd-based catalyst reported. The TOF values (mol of H2 × (mol of catalyst × min)−1) of RGO/Pd is 6.25, which appears to be one of the best catalysts reported so far. We also obtained a 11B NMR spectrum to investigate the mechanism of this catalytic hydrolysis process. This simple and straightforward method is of significance for the facile preparation of metal nanocatalysts with high catalytic activity on proper supporting materials.
Co-reporter:Guoqiang Xie, Ju Cheng, Yifan Li, Pinxian Xi, Fengjuan Chen, Hongyan Liu, Fengping Hou, Yanjun Shi, Liang Huang, Zhihong Xu, Decheng Bai and Zhengzhi Zeng
Journal of Materials Chemistry A 2012 vol. 22(Issue 18) pp:9308-9314
Publication Date(Web):28 Mar 2012
DOI:10.1039/C2JM16678J
In this research, we developed a “clean” and “wet” route to produce sandwich-like fluorescent CdQ2/GO composites sheets starting from graphene oxide (GO) and Cd2+ in water/ethanol solution in one pot. The as-prepared CdQ2/GO composites sheets were fully characterized by IR, XRD, TEM and TGA analysis. The CdQ2/GO composites exhibited a strong fluorescence emission centered at 500 nm. Both the solid sample and the water dispersion could demonstrate strong green fluorescence. When incubated with Hep G2 cells at 37 °C, the CdQ2/GO composites were found to adhere to the surroundings of the cell membrane, resulting in strong green fluorescence observed by laser confocal fluorescence microscopy. MTT assay experiment results showed that this kind of composite material had low cytotoxicity at the concentration of 400 μg mL−1. We believe this method and composite materials will open up a brand new avenue for wider applications of graphene-based materials in the detection of other types of biomarkers, biomolecular interactions, and fluorescence imaging in vitro and in vivo.
Co-reporter:Guoqiang Xie, Pinxian Xi, Hongyan Liu, Fengjuan Chen, Liang Huang, Yanjun Shi, Fengping Hou, Zhengzhi Zeng, Changwei Shao and Jun Wang
Journal of Materials Chemistry A 2012 vol. 22(Issue 3) pp:1033-1039
Publication Date(Web):14 Nov 2011
DOI:10.1039/C1JM13433G
A superparamagnetic graphene oxide–Fe3O4 hybrid composite (GO–Fe3O4) was prepared via a simple and effective chemical method. Amino-functionalized Fe3O4 (NH2-Fe3O4) particles are firmly deposited on the graphene oxide sheets. The graphene oxide sheets could prevent NH2-Fe3O4 particles from agglomeration and enable a good dispersion of these oxide particles. The as-prepared GO–Fe3O4 hybrid composite had a much higher thermal stability than graphene oxide. The amount of NH2-Fe3O4 loaded on GO was estimated to be 23.6 wt% by atomic absorption spectrometry. The specific saturation magnetization (Ms) of the GO–Fe3O4 hybrid composite is 15 emu g−1. The magnetic GO–Fe3O4 composite has been employed as adsorbent for the magnetic separation of dye contaminants from water. The adsorption test of dyes (Methylene Blue (MB) and Neutral Red (NR)) demonstrates that it only takes 30 min for MB and 90 min for NR to attain equilibrium. The adsorption capacities for MB and NR in the concentration range studied are 167.2 and 171.3 mg g−1, respectively. The GO–Fe3O4 hybrid composite can be easily manipulated in magnetic field for desired separation, leading to the removal of dyes from polluted water. These GO–Fe3O4 hybrid composites have great potential applications in removing organic dyes from polluted water.
Co-reporter:Pinxian Xi, Kai Cheng, Xiaolian Sun, Zhengzhi Zeng and Shouheng Sun
Chemical Communications 2012 vol. 48(Issue 24) pp:2952-2954
Publication Date(Web):23 Jan 2012
DOI:10.1039/C2CC18122C
Magnetic 8 nm Fe3O4 nanoparticles (NPs) were synthesized and modified with dopamine (DPA) and polyethylene glycol (PEG) diacid. The water soluble Fe3O4–DPA–PEG NPs were then conjugated with the fluorescent Eu(III) complex of tris(dibenzoylmethane)-5-amino-1,10-phenanthroline (BMAP), giving an Fe3O4–DPA–PEG–BMAP–Eu NP conjugate. The conjugate was both colloidally and chemically stable in phosphate buffered solutions and could be used as a probe for magnetic resonance and fluorescent imaging.
Co-reporter:Fengping Hou ; Liang Huang ; Pinxian Xi ; Ju Cheng ; Xuefei Zhao ; Guoqiang Xie ; Yanjun Shi ; Fengjuan Cheng ; Xiaojun Yao ; Decheng Bai
Inorganic Chemistry 2012 Volume 51(Issue 4) pp:2454-2460
Publication Date(Web):February 3, 2012
DOI:10.1021/ic2024082
A novel selective fluorescent chemosensor based on an 8-hydroxyquinoline-appended fluorescein derivative (L1) was synthesized and characterized. Once combined with Cu2+, it displayed high specificity for sulfide anion. Among the various anions, only sulfide anion induced the revival of fluoresecence of L1, which was quenched by Cu2+, resulting in “off–on”-type sensing of sulfide anion. What’s more, the sensor was retrievable to indicate sulfide anions with Cu2+, and S2–, in turn, increased. With the addition of Cu2+, compound L1 could give rise to a visible pink-to-yellow color change and green fluorescence quenching. The resulting yellow solution could change to pink and regenerate to green fluorescence immediately upon the addition of sulfide anion; however, no changes were observed in the presence of other anions, including CN–, P2O74–, and other forms of sulfate, making compound L1 an extremely selective and efficient sulfide chemosensor. The signal transduction occurs via reversible formation–separation of complex L1Cu and CuS. What’s more, the biological imaging study has demonstrated that the chemosensor can detect sulfur anions in biological systems at a relatively low concentration.
Co-reporter:Guoqiang Xie;Yanjun Shi;Fengping Hou;Hongyan Liu;Liang Huang;Pinxian Xi;Fengjuan Chen
European Journal of Inorganic Chemistry 2012 Volume 2012( Issue 2) pp:327-332
Publication Date(Web):
DOI:10.1002/ejic.201100804
Abstract
A fluorescent, colorimetric chemosensor, L1, based on 8-aminoquinoline, has been developed as a sensor for Zn2+. L1 exhibited high selectivity and sensitivity towards Zn2+ over other common metal ions in a physiological pH window with a 1:1 binding mode. The ability of L1 to monitor intracellular Zn2+ in living cells has been examined by fluorescence microscopy and indicated that L1 is cell-permeable and biocompatible. We hope that such Zn2+-selective sensors will find application in biomedical and environmental detection.
Co-reporter:Hongyan Liu ; Pinxian Xi ; Guoqiang Xie ; Yanjun Shi ; Fengping Hou ; Liang Huang ; Fengjuan Chen ; Zhengzhi Zeng ; Changwei Shao ;Jun Wang
The Journal of Physical Chemistry C 2012 Volume 116(Issue 5) pp:3334-3341
Publication Date(Web):January 3, 2012
DOI:10.1021/jp2102226
In this study, we present the synthesis of reduced graphene oxide/hydroxyapatite (RGO/HA) hybrid materials by an environmental-friendly route. Graphene oxide (GO) was first simultaneously reduced and surface functionalized by one-step oxidative polymerization of dopamine (PDA). The bioinspired surface was further used for biomimetic mineralization of hydroxyapatite. When incubated in a simulated body fluid (SBF), the PDA layer enabled efficient interaction between the RGO surface and the mineral ions to improve the bioactivity, promoted the formation of the HA nanoparticles. A detailed structural and morphological characterization of the mineralized composite was performed. The HA-based hybrid materials exhibited no cytotoxic effect on L929 fibroblast cells, showing potential capacity of being a scaffold material for bone tissue regeneration and implantation. This facile strategy also can be a useful platform for other RGO-based nanocomposites.
Co-reporter:Pinxian Xi, Kai Cheng, Xiaolian Sun, Zhengzhi Zeng and Shouheng Sun
Journal of Materials Chemistry A 2011 vol. 21(Issue 31) pp:11464-11467
Publication Date(Web):03 Dec 2010
DOI:10.1039/C0JM03119D
A fluorescent ruthenium (Ru) complex is coupled to magnetic Fe3O4 nanoparticles (NPs) via3-(3,4-dihydroxyphenyl) propanoic acid (DHPPA) and O,O′-bis(2-aminopropyl) polypropylene glycol-block-polyethylene glycol-block-polypropylene glycol (PPG-PEG-PPG-diamine). The resultant Ru–Fe3O4 NP conjugate shows excellent colloidal, photochemical and magnetic stability, and is promising as a dual functional probe for biological imaging applications.
Co-reporter:Pinxian Xi, Liang Huang, Guoqiang Xie, Fengjuan Chen, Zhihong Xu, Decheng Bai and Zhengzhi Zeng
Dalton Transactions 2011 vol. 40(Issue 24) pp:6382-6384
Publication Date(Web):16 May 2011
DOI:10.1039/C1DT10573F
A turn-on chemosensor L1, which exhibits high selectivity and sensitivity toward Hg2+ over other common metal ions in aqueous media under a physiological pH window via a 1:1 binding mode, had been synthesized and characterized. L1 provides good fluorescent imaging of Hg2+ in living cells. Particularly, we adopted the “micro computed tomography (MCT)” technology, successfully demonstrating the method of Hg2+ sensing by L1 in cell lines, also the cell permeability of L1 and its imaging position in the cells.
Co-reporter:Hongyan Liu ; Fengjuan Chen ; Pinxian Xi ; Bin Chen ; Liang Huang ; Ju Cheng ; Changwei Shao ; Jun Wang ; Decheng Bai
The Journal of Physical Chemistry C 2011 Volume 115(Issue 38) pp:18538-18544
Publication Date(Web):August 24, 2011
DOI:10.1021/jp206843w
Various fluorescent nanoparticles have been applied in imaging of tissues or intracellular structures. Searching for biocompatible nanoparticles that can be observed under visible light is of fundamental importance for the applications in living cells. In this study, a simple, template-based route for the synthesis of fluorescent hydroxyapatite (HA) nanoparticles has been developed. Well-dispersed HA nanoparticles prepared by using SiO2 as templates (THA) were surface-functionalized with fluorescein isothiocyanate (FITC), resulting in bright and stable fluorescent particles (THA-FITC). These fluorescent nanoparticles were demonstrated to be internalized into Hela cells and have no apparent cytotoxic effects on fibroblast cells, which confirmed the biocompatibility and cell labeling capability of the nanoparticles. Due to the large surface areas, porous nature, and visible light excited luminescence characters, THA-FITC nanoparticles will have applications in fields such as cell labeling, whole animal imaging and therapy, and drug delivery and release.
Co-reporter:Hongyan Liu;Pinxian Xi;Guoqiang Xie
JBIC Journal of Biological Inorganic Chemistry 2011 Volume 16( Issue 8) pp:1135-1140
Publication Date(Web):2011/12/01
DOI:10.1007/s00775-011-0815-6
A redox luminescence switch was prepared by doping hydroxyapatite nanoparticles with CePO4:Tb. The resulting multifunctional material exhibits good biocompatibility, biological affinity, and potential drug-carrying capability. The luminescent hydroxyapatite nanoparticles may find important applications in biomedical diagnostics, drug delivery, and biological sensors.
Co-reporter:Zong-Lian DUAN;Zheng-Zhi ZENG
Chinese Journal of Chemistry 2007 Volume 25(Issue 12) pp:
Publication Date(Web):12 DEC 2007
DOI:10.1002/cjoc.200790353
N1,N1-Dimethylbiguanide hydrochloride (HDMBG·HCl) was introduced as an oral glucose-lowering agent to treat non-insulin dependent diabetes mellitus. In this paper, two solid complexes–trichlorotris(N5-benzoyl-N1,N1-dimethylbiguanidato)neodymium, [Nd(BDMBG)3Cl3), and chlorobis-[N5-(o-carboxybenzoyl)-N1,N1- dimethylbiguanidato]neodymium, [Nd(CDMBG)2Cl], were synthesized and characterized by elemental analyses, fluorescence spectra, IR, UV, DG, DTA etc. Biological testings were conducted by diabetic rats and ESR The results showed that both the antidiabetic activity and the inhibition rates (Ih) to superoxide anion free radical O2− on lipid are in the order as follows: Nd(SDMBG)3 [tris(N5-salicyloyl-N1,N1-dimethylbiguanidato)neodymium]>Nd(CDMBG)2Cl≈HDMBG·HCl>Nd(BDMBG)3Cl3. It was concluded that different substituents at the o-position of the benzoyl group may affect these two kinds of behaviour to a great extent.
Co-reporter:Xiao-Hui Liu;Yan Ye;Zheng-Zhi Zeng
Chinese Journal of Chemistry 2007 Volume 25(Issue 2) pp:
Publication Date(Web):7 FEB 2007
DOI:10.1002/cjoc.200790037
The interaction of gatifloxacin (HGA) with bovine serum albumin (BSA) at 15 and 37 °C has been investigated by fluorescence quenching spectroscopy in aqueous solution. The bimolecular quenching rate constant was determined by Stern-Volmer curves and the values were Kq=9.28×1012 L·mol−1·s−1 (15 °C) and Kq=8.51×1012 L·mol−1·s−1 (37 °C). The results showed that the fluorescence quenching mechanism of BSA by HGA was a static quenching procedure. The thermodynamic parameters indicated that electrostatic forces played major role in the interaction of BSA with HGA. Studies on the relationship between the concentration of HGA and the fluorescence intensity of BSA >showed that BSA and HGA bound at the molar ratio 1:1 and the equilibrium constant K0 was 6.80×104 L·mol−1. The binding distances between BSA and HGA and the energy transfer efficiency were obtained based on the FÖrster's theory.
Co-reporter:Hui-Li Lu;Jian-Jun Liang;Zheng-Zhi Zeng;Pin-Xian Xi
Transition Metal Chemistry 2007 Volume 32( Issue 5) pp:564-569
Publication Date(Web):2007 August
DOI:10.1007/s11243-007-0202-x
Three new Schiff base ligands N-(3-formyl-5-methylsalicylidene)-2-aminoethanol (H2L1), N-(3-hydroxylmethyl-5-methylsalicylidene)-2-aminoethanol (H3L2), 2,6-bis(o-carboxyphenyliminomethene)-4-methylphenol (H3L3) and their binuclear ZnII complexes [Zn2(HL1)2]Cl2 · 2H2O (ZnHL1), [Zn2(H2L2)2]Cl2 · H2O (ZnH2L2) and [Zn2(HL3)Cl2] · H2O (ZnHL3) were synthesized and characterized by 1H-NMR, elemental analysis, IR and molar conductivity. The results suggest, in every case, two Zn2+ ions were bridged by phenolic OH group oxygen, forming a binuclear complex. The binding properties of these complexes to calf thymus DNA (ct-DNA) were investigated. Absorption and fluorescence spectra are together suggestive that both ZnHL1 and ZnHL3 interact with ct-DNA through intercalative mode, while ZnH2L2 interact with ct-DNA by non-intercalative interaction. Moreover, ZnHL3 can bind to ct-DNA more strongly than ZnHL1. These complexes also exhibited good scavenging activity on the hydroxyl radical (•OH), which are better than those of their corresponding ligands.
Co-reporter:Pin-xian Xi;Xiao-hui Liu;Hui-li Lu;Zheng-zhi Zeng
Transition Metal Chemistry 2007 Volume 32( Issue 6) pp:757-761
Publication Date(Web):2007 September
DOI:10.1007/s11243-007-0244-0
1-(Pyridin-2-yl)-3-tosylurea(HL) and its two lanthanide complexes, LnL3 · H2O [Ln = Nd(1), Eu(2)], have been synthesized and characterized on the base of elemental analyses, molar conductivities, IR spectra and thermal analyses. In addition, the DNA-binding properties of the two complexes have been investigated by absorption, fluorescence and viscosity measurements. The experimental results indicated that the complex (1) and (2) can bind to DNA; the binding affinity of the complex (1) is higher than that of complex (2) and the intrinsic binding constants of complex (1) and complex (2) with DNA were 1.24 × 104 m−1 and 8.7 × 103 m−1, respectively. In addition, some factors that influence the fluorescent intensity were also discussed.
Co-reporter:Pinxian Xi, Kai Cheng, Xiaolian Sun, Zhengzhi Zeng and Shouheng Sun
Chemical Communications 2012 - vol. 48(Issue 24) pp:NaN2954-2954
Publication Date(Web):2012/01/23
DOI:10.1039/C2CC18122C
Magnetic 8 nm Fe3O4 nanoparticles (NPs) were synthesized and modified with dopamine (DPA) and polyethylene glycol (PEG) diacid. The water soluble Fe3O4–DPA–PEG NPs were then conjugated with the fluorescent Eu(III) complex of tris(dibenzoylmethane)-5-amino-1,10-phenanthroline (BMAP), giving an Fe3O4–DPA–PEG–BMAP–Eu NP conjugate. The conjugate was both colloidally and chemically stable in phosphate buffered solutions and could be used as a probe for magnetic resonance and fluorescent imaging.
Co-reporter:Pinxian Xi, Liang Huang, Guoqiang Xie, Fengjuan Chen, Zhihong Xu, Decheng Bai and Zhengzhi Zeng
Dalton Transactions 2011 - vol. 40(Issue 24) pp:NaN6384-6384
Publication Date(Web):2011/05/16
DOI:10.1039/C1DT10573F
A turn-on chemosensor L1, which exhibits high selectivity and sensitivity toward Hg2+ over other common metal ions in aqueous media under a physiological pH window via a 1:1 binding mode, had been synthesized and characterized. L1 provides good fluorescent imaging of Hg2+ in living cells. Particularly, we adopted the “micro computed tomography (MCT)” technology, successfully demonstrating the method of Hg2+ sensing by L1 in cell lines, also the cell permeability of L1 and its imaging position in the cells.
Co-reporter:Pinxian Xi, Kai Cheng, Xiaolian Sun, Zhengzhi Zeng and Shouheng Sun
Journal of Materials Chemistry A 2011 - vol. 21(Issue 31) pp:NaN11467-11467
Publication Date(Web):2010/12/03
DOI:10.1039/C0JM03119D
A fluorescent ruthenium (Ru) complex is coupled to magnetic Fe3O4 nanoparticles (NPs) via3-(3,4-dihydroxyphenyl) propanoic acid (DHPPA) and O,O′-bis(2-aminopropyl) polypropylene glycol-block-polyethylene glycol-block-polypropylene glycol (PPG-PEG-PPG-diamine). The resultant Ru–Fe3O4 NP conjugate shows excellent colloidal, photochemical and magnetic stability, and is promising as a dual functional probe for biological imaging applications.
Co-reporter:Guoqiang Xie, Pinxian Xi, Hongyan Liu, Fengjuan Chen, Liang Huang, Yanjun Shi, Fengping Hou, Zhengzhi Zeng, Changwei Shao and Jun Wang
Journal of Materials Chemistry A 2012 - vol. 22(Issue 3) pp:NaN1039-1039
Publication Date(Web):2011/11/14
DOI:10.1039/C1JM13433G
A superparamagnetic graphene oxide–Fe3O4 hybrid composite (GO–Fe3O4) was prepared via a simple and effective chemical method. Amino-functionalized Fe3O4 (NH2-Fe3O4) particles are firmly deposited on the graphene oxide sheets. The graphene oxide sheets could prevent NH2-Fe3O4 particles from agglomeration and enable a good dispersion of these oxide particles. The as-prepared GO–Fe3O4 hybrid composite had a much higher thermal stability than graphene oxide. The amount of NH2-Fe3O4 loaded on GO was estimated to be 23.6 wt% by atomic absorption spectrometry. The specific saturation magnetization (Ms) of the GO–Fe3O4 hybrid composite is 15 emu g−1. The magnetic GO–Fe3O4 composite has been employed as adsorbent for the magnetic separation of dye contaminants from water. The adsorption test of dyes (Methylene Blue (MB) and Neutral Red (NR)) demonstrates that it only takes 30 min for MB and 90 min for NR to attain equilibrium. The adsorption capacities for MB and NR in the concentration range studied are 167.2 and 171.3 mg g−1, respectively. The GO–Fe3O4 hybrid composite can be easily manipulated in magnetic field for desired separation, leading to the removal of dyes from polluted water. These GO–Fe3O4 hybrid composites have great potential applications in removing organic dyes from polluted water.
Co-reporter:Guoqiang Xie, Ju Cheng, Yifan Li, Pinxian Xi, Fengjuan Chen, Hongyan Liu, Fengping Hou, Yanjun Shi, Liang Huang, Zhihong Xu, Decheng Bai and Zhengzhi Zeng
Journal of Materials Chemistry A 2012 - vol. 22(Issue 18) pp:NaN9314-9314
Publication Date(Web):2012/03/28
DOI:10.1039/C2JM16678J
In this research, we developed a “clean” and “wet” route to produce sandwich-like fluorescent CdQ2/GO composites sheets starting from graphene oxide (GO) and Cd2+ in water/ethanol solution in one pot. The as-prepared CdQ2/GO composites sheets were fully characterized by IR, XRD, TEM and TGA analysis. The CdQ2/GO composites exhibited a strong fluorescence emission centered at 500 nm. Both the solid sample and the water dispersion could demonstrate strong green fluorescence. When incubated with Hep G2 cells at 37 °C, the CdQ2/GO composites were found to adhere to the surroundings of the cell membrane, resulting in strong green fluorescence observed by laser confocal fluorescence microscopy. MTT assay experiment results showed that this kind of composite material had low cytotoxicity at the concentration of 400 μg mL−1. We believe this method and composite materials will open up a brand new avenue for wider applications of graphene-based materials in the detection of other types of biomarkers, biomolecular interactions, and fluorescence imaging in vitro and in vivo.
