Co-reporter:Lei Zhou, Shouguo Wu, Haihong Xu, Qiping Zhao, Zhixin Zhang and Yao Yao
Analytical Methods 2014 vol. 6(Issue 19) pp:8003-8010
Publication Date(Web):07 Aug 2014
DOI:10.1039/C4AY01018C
A poly(N-acetylaniline)–Prussian blue (PNAANI–PB) hybrid composite film was prepared by co-electrodeposition. Using a spontaneous redox reaction, the PNAANI–PB hybrid film was prepared electrochemically by cyclic voltammetry in an electroplating bath containing a low concentration of N-acetylaniline. Its surface morphology showed that the PB nanoparticles in the hybrid film had a smaller size than those in the pure PB film. As a result of electrostatic interaction between the PNAANI and the PB particles, the PNAANI–PB hybrid film displays impressive synergistic effects that remarkably enhance its sensitivity, expand its linear range and broaden its acidic adaptability in the detection of H2O2. The hybrid film displays good stability in neutral solution, in contrast to the pure PB film, with a linear range from 10−6 to 10−3 M and a high sensitivity of 507.29 μA mM−1 cm−2 for H2O2 detection. An amperometric glucose biosensor was constructed by immobilizing glucose oxidase on the hybrid film. The biosensor showed an excellent response to glucose in the linear range 10−5–10−3 M and had a high sensitivity of 62.45 μA mM−1 cm−2. The glucose biosensor had a rapid response, good reproducibility and long-term stability and was free of interference from other coexisting electroactive species.
Co-reporter:Shouguo Wu, Gang Liu, Ping Li, Hao Liu, Haihong Xu
Biosensors and Bioelectronics 2012 Volume 38(Issue 1) pp:289-294
Publication Date(Web):October–December 2012
DOI:10.1016/j.bios.2012.06.001
A novel and fast-fabricated Prussian blue (PB)/topological insulator Bi2Se3 hybrid film has been prepared by coelectrodeposition technique. Taking advantages of topological insulator in possessing exotic metallic surface states with bulk insulating gap, Prussian blue nanoparticles in the hybrid film have smaller size as well as more compact structure, showing excellent pH stability even in the alkalescent solution of pH 8.0. Based on the Laviron theory, the electron transfer rate constant of PB/Bi2Se3 hybrid film modified electrode was calculated to be 4.05±0.49 s−1, a relatively big value which may be in favor of establishing a high-sensitive biosensor. An amperometric glucose biosensor was then fabricated by immobilizing glucose oxidase (GOD) on the hybrid film. Under the optimal conditions, a wide linear range extending over 3 orders of magnitude of glucose concentrations (1.0×10−5–1.1×10−2 M) was obtained with a high sensitivity of 24.55 μA mM−1 cm−2. The detection limit was estimated for 3.8 μM defined from a signal/noise of 3. Furthermore, the resulting biosensor was applied to detect the blood sugar in human serum samples without any pretreatment, and the results were comparatively in agreement with the clinical assay.Highlights► Topological insulator (TI) Bi2Se3 has metallic surface with insulating bulk and we apply it. ► Coelectrodepositon was used to form PB/Bi2Se3 hybrid film, playing the role of TI. ► Bi2Se3 is conducive to form smaller PB nanoparticles and a smoother hybrid film. ► The hybrid film show excellent pH stability even in the alkalescent solution of pH 8. ► Fabrication of the hybrid film based biosensor is fast and it has potential practical utility.
Co-reporter:Shouguo Wu, Wenguang Tan and Haihong Xu
Analyst 2010 vol. 135(Issue 10) pp:2523-2527
Publication Date(Web):13 Jul 2010
DOI:10.1039/C0AN00191K
A hemoglobin sensor based on a molecularly imprinted polymer-modified electrode has been fabricated by electrochemically induced redox polymerization of acrylamide in the presence of hemoglobin.
Co-reporter:Shouguo Wu;Chuanqin Wang;Changqin Wang;Xian Zhang;Taoling Wang
Polymer Composites 2008 Volume 29( Issue 10) pp:1152-1159
Publication Date(Web):
DOI:10.1002/pc.20409
Abstract
Electrochemical preparation of the Prussian blue (PB)/poly (N-acetylaniline) (PNAANI) composite film was carried out by cyclic voltammetry (CV). Its surface morphology, which was evaluated by Atom Force Microscope (AFM), revealed that PNAANI film provides lots of redox centers for PB growth so that the electrodeposition of PB showed high current densities, and the composite film is much denser than pure PB film. The surface microstructure of the composite film was also characterized by X-ray photoelectron spectrum (XPS). Due to the existence of electrostatic interaction between the PNAANI (polycation) film and the PB (polyanion) film, the composite film cannot be considered as a simple adherent bilayer of PB film and PNAANI film. The electroactivity and stability of the PB/PNAANI composite film in acidic electrolytes containing potassium ions were comparatively higher than that of the pure PB film. The functional composite film is expected for use in the system capable of effective accumulation of charge, and for the analytical applications towards the reduction of hydrogen peroxide due to its high electrocatalytic activity. POLYM. COMPOS., 2008. © 2008 Society of Plastics Engineers
Co-reporter:Baineng Zhou
Journal of Applied Polymer Science 2008 Volume 109( Issue 4) pp:2400-2407
Publication Date(Web):
DOI:10.1002/app.28309
Abstract
Poly(N-acetylaniline)/poly(4-styrenesulfonic acid-co-maleic acid) (PNAANI/PSSMA) composite film was prepared by cyclic voltammetry (CV), and was characterized by FTIR and X-ray photoelectron spectrum (XPS). The electroactivity of the composite film was high in neutral and basic solutions, and it had been used for amperometric determination of ascorbic acid (AA). Compared with pure PNAANI film, the catalytic activity of the composite film was much better. AA was detected amperometrically in sodium citrate buffer at a potential of 0.3 V (versus SCE). The response current was proportional to the concentration of ascorbic acid in the range of 4.7 × 10−6 to 5.0 × 10−5M and 5.0 × 10−5 to 2.5 × 10−3M, respectively, with the detection limit of 1.9 × 10−6 mol L−1 at a signal to noise ratio 3. In addition, the stability and reusability of the composite film were performed well, and it was satisfying to be used for determination of AA in real fruit juice samples. © 2008 Wiley Periodicals, Inc. J Appl Polym Sci, 2008
Co-reporter:Shouguo Wu, Taoling Wang, Zongyong Gao, Haihong Xu, Baineng Zhou, Chuanqin Wang
Biosensors and Bioelectronics 2008 Volume 23(Issue 12) pp:1776-1780
Publication Date(Web):15 July 2008
DOI:10.1016/j.bios.2008.02.012
A β-cyclodextrin (CD) modified copolymer membrane of sulfanilic acid (p-ASA) and N-acetylaniline (SPNAANI) on glassy carbon electrode (GCE) was prepared and used to determine uric acid (UA) in the presence of a large excess of ascorbic acid (AA) by differential pulse voltammetry (DPV). The properties of the copolymer were characterized by X-ray photoelectron spectra (XPS) and Raman spectroscopy. The oxidation peaks of AA and UA were well separated at the composite membrane modified electrode in phosphate buffer solution (PBS, pH 7.4). A linear relationship between the peak current and the concentration of UA was obtained in the range from 1.0 × 10−5 to 3.5 × 10−4 mol L−1, and the detection limit was 2.7 × 10−6 mol L−1 at a signal-to-noise ratio of 3. Two hundred and fifty-fold excess of AA did not interfere with the determination of UA. The application of the prepared electrode was demonstrated by measuring UA in human serum samples without any pretreatment, and the results were comparatively in agreement with the spectrometric clinical assay method.
Co-reporter:Meiju Du, Xiaogang Han, Zihao Zhou, Shouguo Wu
Food Chemistry 2007 Volume 105(Issue 2) pp:883-888
Publication Date(Web):2007
DOI:10.1016/j.foodchem.2006.12.039
A novel electroanalytical method is proposed for the determination of Sudan I in hot chili powder. Sudan I was firstly pre-concentrated by adsorption and then electroreduced at the electrochemically activated glassy carbon electrode (AGCE). A linear relationship between the reduction current and concentration of Sudan I was obtained over the range from 2.4 × 10−6 mol/L to 1.8 × 10−5 mol/L with a correlation coefficient of 0.9981. The detection limit was estimated to be about 7.1 × 10−7 mol/L.
Co-reporter:Xianbo Lu, Shouguo Wu, Li Wang, Zhenxi Su
Sensors and Actuators B: Chemical 2005 Volume 107(Issue 2) pp:812-817
Publication Date(Web):29 June 2005
DOI:10.1016/j.snb.2004.12.022
An amperometric hydrogen sensor of whole solid-state has been fabricated. The sensor based on polymer electrolyte membrane fuel cell (PEMFC) uses Nafion membrane as proton-conducting solid polymer electrolyte (SPE). It adopts three-electrode mode that consists of three gas-diffusion electrodes. The sensor can work at ambient temperature. When a fixed potential of 0.15 V is imposed on the sensor, the steady-state currents respond linearly to the concentrations of hydrogen from 560 to 11,500 ppm (v/v). The factors that influence the performance of the sensor are investigated and discussed. The simple miniature hydrogen sensor prepared shows good sensitivity, short response time, wide linear range and long-term stability. It can be used to monitor hydrogen concentrations in gas mixtures on-line. If the sensor is improved to achieve a lower detection limit, it may be applied to detect hydrogen in aqueous solution.
Co-reporter:Longzhen Zheng, Shouguo Wu, Xiangqin Lin, Lei Nie and Lei Rui
Analyst 2001 vol. 126(Issue 6) pp:736-738
Publication Date(Web):14 May 2001
DOI:10.1039/B102534C
An over-oxidized poly(N-acetylaniline) (PNAANI)/GCE was used to determine dopamine (DA) in a large excess of ascorbic acid (AA) by differential pulse voltammetry. A linear relation between Ip and DA concentration was found over the range 5.0 × 10−7 to 2.0 × 10−5 M. The detection limit was 1.68 × 10−8 M for S/N = 3 and 400 µM AA did not interfere with the DA determination. The high sensitivity was due to accumulation and selectivity was due to charge discrimination. The mechanism of selective determination of DA at over-oxidized PNAANI/GCE was also proposed.
Co-reporter:Shouguo Wu, Lei Nie, Jianwei Wang, Xiangqin Lin, Longzhen Zheng, Lei Rui
Journal of Electroanalytical Chemistry 2001 Volume 508(1–2) pp:11-27
Publication Date(Web):27 July 2001
DOI:10.1016/S0022-0728(01)00526-5
To separate the overlapping voltammetric peaks that can be described by the sech2-function, a new method, flip shift subtraction method (FSSM), is proposed. It is built on the basis of finding the peak positions using the Marr wavelet through the continuous wavelet transform (CWT). A corresponding theory, which possesses a general meaning, is presented as a basis of finding the peak positions. To guarantee the accuracy of FSSM, an algorithm, crossed iterative algorithm of continuous wavelet transform and original signal (CIACWTOS), is built on to locate the refined peak positions. Meanwhile, the theoretical errors on peak areas caused by FSSM are discussed.
Co-reporter:
Analytical Methods (2009-Present) 2014 - vol. 6(Issue 19) pp:
Publication Date(Web):
DOI:10.1039/C4AY01018C
A poly(N-acetylaniline)–Prussian blue (PNAANI–PB) hybrid composite film was prepared by co-electrodeposition. Using a spontaneous redox reaction, the PNAANI–PB hybrid film was prepared electrochemically by cyclic voltammetry in an electroplating bath containing a low concentration of N-acetylaniline. Its surface morphology showed that the PB nanoparticles in the hybrid film had a smaller size than those in the pure PB film. As a result of electrostatic interaction between the PNAANI and the PB particles, the PNAANI–PB hybrid film displays impressive synergistic effects that remarkably enhance its sensitivity, expand its linear range and broaden its acidic adaptability in the detection of H2O2. The hybrid film displays good stability in neutral solution, in contrast to the pure PB film, with a linear range from 10−6 to 10−3 M and a high sensitivity of 507.29 μA mM−1 cm−2 for H2O2 detection. An amperometric glucose biosensor was constructed by immobilizing glucose oxidase on the hybrid film. The biosensor showed an excellent response to glucose in the linear range 10−5–10−3 M and had a high sensitivity of 62.45 μA mM−1 cm−2. The glucose biosensor had a rapid response, good reproducibility and long-term stability and was free of interference from other coexisting electroactive species.
![Benzenemethanesulfonicacid, 3,4-dihydroxy-a-[(methylamino)methyl]-](http://img.cochemist.com/ccimg/26500/26405-77-6.png)
![Benzenemethanesulfonicacid, 3,4-dihydroxy-a-[(methylamino)methyl]-](http://img.cochemist.com/ccimg/26500/26405-77-6_b.png)
