Co-reporter:Ziru Lian, Jiangtao Wang
Marine Pollution Bulletin 2017 Volume 122, Issues 1–2(Issue 1) pp:
Publication Date(Web):15 September 2017
DOI:10.1016/j.marpolbul.2017.06.066
•The molecularly imprinted polymer microspheres (MIPMs) for GTX1,4 were prepared.•An off-line method using MIPMs as solid-phase extraction (SPE) sorbents was developed.•GTX1,4 from Alexandrium minutum extract was successfully isolated by MIPMs-SPE.Gonyautoxins 1,4 (GTX1,4) from Alexandrium minutum samples were isolated selectively and recognized specifically by an innovative and effective extraction procedure based on molecular imprinting technology. Novel molecularly imprinted polymer microspheres (MIPMs) were prepared by double-templated imprinting strategy using caffeine and pentoxifylline as dummy templates. The synthesized polymers displayed good affinity to GTX1,4 and were applied as sorbents. Further, an off-line molecularly imprinted solid-phase extraction (MISPE) protocol was optimized and an effective approach based on the MISPE coupled with HPLC-FLD was developed for selective isolation of GTX1,4 from the cultured A. minutum samples. The separation method showed good extraction efficiency (73.2–81.5%) for GTX1,4 and efficient removal of interferences matrices was also achieved after the MISPE process for the microalgal samples. The outcome demonstrated the superiority and great potential of the MISPE procedure for direct separation of GTX1,4 from marine microalgal extracts.
Co-reporter:Xiuping He, Jianlei Chen, Jiangtao Wang, Liju Tan
Journal of Chromatography A 2017 Volume 1500(Volume 1500) pp:
Publication Date(Web):2 June 2017
DOI:10.1016/j.chroma.2017.04.023
•Different compounds were investigated as dummy templates to synthesize DA-MIPs.•CA as dummy template achieved the most adsorption amount.•The obtained polymer was proved to have high binding capacity and good selectivity.•The MISPE was successfully applied to the efficient extraction of DA in seawater.•Hydrophilicity of CA was proved to be the most important factor for high adsorption.Due to the high cost of domoic acid (DA), different carboxylic acid compounds including indole-3-acetic acid (IAA), pyrrole-2-formic acid (PFA), pyridine-2,3-dicarboxylic anhydride (PDA), trimesitinic acid (TA) and citric acid (CA) were investigated as dummy templates for the molecularly imprinted solid-phase extraction (MISPE) for selective isolation and pre-concentration of an amnesic shellfish poison (ASP), DA. The highest binding amount of the polymers towards DA was obtained when CA was used as dummy template owing to its high hydrophilicity. In addition, the “four-point” recognition site constructed by three COOH groups and a OH group in CA was also speculated to be the reason for the high binding amount of CA-MIPs and the rebinding of DA can be depend on the three COOH groups and a NH group with conformational change in the recognition process. Finally, the CA-MISPE column was chosen for DA isolation and pre-concentration and effective result was obtained with recoveries higher than 90% and relative standard deviation (RSD) less than 5% (n = 3). This new polymer can be effectively applied to the monitoring and predicting the existence of trace DA.
Co-reporter:Rui Wang, Qiaona Xue, Jiangtao Wang, Liju Tan, Qingchun Zhang, Yue Zhao, Donald M. Anderson
Chemosphere 2017 Volume 186(Volume 186) pp:
Publication Date(Web):1 November 2017
DOI:10.1016/j.chemosphere.2017.08.024
•An allelochemical isolated from filtrate of Phaeodactylum tricornutum inhibited the growth of Heterosigma akashiwo.•Allelopathic effects were evaluated using the SEM and FCM as convenient diagnostic approaches.•The allelochemical damaged the membrane integrity and affected the esterase activity of H. akashiwo.•Esterase activity was the most sensitive indicator of the impacts of the allelochemical.The effects of an allelochemical extracted from the culture filtrate of diatom Phaeodactylum tricornutum on the raphidophyte Heterosigma akashiwo were investigated using a series of morphological, physiological and biochemical characters. Growth experiments showed that H. akashiwo was significantly inhibited immediately after exposure to the allelochemical, with many cells rapidly dying and lysing based on microscopic observation. The effects of the allelochemical on the surviving cells were explored using Scanning Electron Microscopy (SEM) and Flow cytometry (FCM), the latter by examination of a suite of physiological parameters (membrane integrity, esterase activity, chlorophyll-a content, membrane potential). The results demonstrate that the membrane of H. akashiwo was attacked by the allelochemical directly, causing cell membrane breakage and loss of integrity. Esterase activity was the most sensitive indicator of the impacts of the allelochemical. Membrane potential and chlorophyll-a content both showed significant decreases following exposure of the Heterosigma cells to high concentrations of the allelochemical for 5 and 6 days. Both were affected, but the membrane potential response was more gradual compared to other effects. The cell size of H. akashiwo did not change compared with the control group. The surviving cells were able to continue to grow and in a few days, re-establish a successful culture, even in the presence of residual allelochemical, suggesting either development of cellular resistance, or the degradation of the chemical.
Co-reporter:Cai Zhang, Xiaohua Chen, Jiangtao Wang, Liju Tan
Environmental Pollution 2017 Volume 220, Part B(Volume 220, Part B) pp:
Publication Date(Web):1 January 2017
DOI:10.1016/j.envpol.2016.11.005
•The toxicity of microplastic depended on their particle size.•The microplastic had negative effects on growth and photosynthesis of microalgae.•Shading effect was not one of reasons for toxic effects of microplastic in this study.•Interactions between microplastic and microalgae observed with SEM technique were direct and reasonable explanations for toxic effects of microplastic.To investigate toxic effects of microplastic on marine microalgae Skeletonema costatum, both algal growth inhibition test and non-contact shading test were carried out, and algal photosynthesis parameters were also determined. The SEM images were used to observe interactions between microplastic and algae. It was found that microplastic (mPVC, average diameter 1 μm) had obvious inhibition on growth of microalgae and the maximum growth inhibition ratio (IR) reached up to 39.7% after 96 h exposure. However, plastic debris (bPVC, average diameter 1 mm) had no effects on growth of microalgae. High concentration (50 mg/L) mPVC also had negative effects on algal photosynthesis since both chlorophyll content and photosynthetic efficiency (ΦPSⅡ) decreased under mPVC treatments. Shading effect was not one reason for toxicity of microplastic on algae in this study. Compared with non-contact shading effect, interactions between microplastic and microalage such as adsorption and aggregation were more reasonable explanations for toxic effects of microplastic on marine microalgae. The SEM images provided a more direct and reasonable method to observe the behaviors of microplastic.Download high-res image (287KB)Download full-size image
Co-reporter:Xiuping He;Liju Tan;Wei Wu
Journal of Separation Science 2016 Volume 39( Issue 11) pp:2204-2212
Publication Date(Web):
DOI:10.1002/jssc.201600233
The development of a simple and effective method for the isolation and purification of sulfadiazine residues in food of animal origin is of great significance since it is a great danger to human health. An off-line molecularly imprinted solid-phase extraction with high-performance liquid chromatography method was proposed for the selective pretreatment and determination of sulfadiazine in eggs, rapidly and effectively. The molecularly imprinted polymer was proved to have a homogeneous spherical structure and porous surface morphology with excellent adsorption capacity of 5258 μg/g for sulfadiazine. The newly established method showed a good linearity in the range of 0–200 μg/L, low limits of detection (0.06 μg/L), acceptable reproducibility (RSD, 2.60–5.03%, n = 3), and satisfactory relative recoveries (78.22–86.10%). It was demonstrated that the proposed molecularly imprinted solid-phase extraction with high-performance liquid chromatography method could be applied to determine sulfadiazine in eggs, which simplified the pretreatment procedure and improved the accuracy of the analysis process by reducing the loss of sulfadiazine in the fat-removing procedure compared with traditional methods. Molecularly imprinted solid-phase extraction with excellent selectivity and adsorption capacity is a simple, rapid, selective, and effective pretreatment method for the determination of sulfadiazine in egg samples.
Co-reporter:Xiao-Qi Mei;Xiu-Ping He;Jiang-Tao Wang
Analytical and Bioanalytical Chemistry 2016 Volume 408( Issue 21) pp:5737-5743
Publication Date(Web):2016 August
DOI:10.1007/s00216-016-9675-9
A kind of new molecularly imprinted polymer (MIP) was synthesized by bulk polymerization using guanosine as dummy template molecule, α-methacrylic acid as functional monomer and ethylene glycol dimethyl acrylic ester as crosslinker. Fourier transform infrared spectroscopy (FT-IR) and scanning electron microscopy (SEM) showed that the MIP had homogenous and uniform-sized cavities. It was confirmed that the MIP had higher binding affinity and selectivity towards gonyautoxins 1,4 (GTX 1,4) than the non-imprinted polymer (NIP) according to the static equilibrium adsorption. An off-line molecularly imprinted solid-phase extraction (MISPE) method followed by high-performance liquid chromatography with fluorescence detection (HPLC-FLD) was established for the analysis of GTX 1,4. 0.1 mol/L acetic acid and 95:5 (v:v) methanol/water were optimized as the washing and elution solutions, respectively. The recoveries of spiked cultured seawater samples were satisfactory, as high as 88 %. Using this method, the concentrations of GTX 1,4 from cultured seawater samples of Alexandrium minutum and Alexandrium tamarense were detected to be 1.10 μg/L and 0.99 μg/L, respectively.
Co-reporter:Ziru Lian;Zhenlin Liang
Journal of Separation Science 2015 Volume 38( Issue 20) pp:3655-3660
Publication Date(Web):
DOI:10.1002/jssc.201500663
This research highlights the application of highly efficient molecularly imprinted solid-phase extraction for the preconcentration and analysis of melamine in aquaculture feed samples. Melamine-imprinted polymers were synthesized employing methacrylic acid and ethylene glycol dimethacrylate as functional monomer and cross-linker, respectively. The characteristics of obtained polymers were evaluated by scanning electron microscopy, Fourier transform infrared spectroscopy and binding experiments. The imprinted polymers showed an excellent adsorption ability for melamine and were applied as special solid-phase extraction sorbents for the selective cleanup of melamine. An off-line molecularly imprinted solid-phase extraction procedure was developed for the separation and enrichment of melamine from aquaculture feed samples prior to high-performance liquid chromatography analysis. Optimum molecularly imprinted solid-phase extraction conditions led to recoveries of the target in spiked feed samples in the range 84.6–96.6% and the relative standard deviation less than 3.38% (n = 3). The aquaculture feed sample was determined, and there was no melamine found. The results showed that the molecularly imprinted solid-phase extraction protocols permitted the sensitive, uncomplicated and inexpensive separation and pre-treatment of melamine in aquaculture feed samples.
Co-reporter:Ziru Lian, Xingliang He, Jiangtao Wang
Journal of Chromatography B 2014 Volume 957() pp:53-59
Publication Date(Web):15 April 2014
DOI:10.1016/j.jchromb.2014.02.053
•The sulfadiazine molecularly imprinted polymer (SDZ-MIP) was prepared.•The characteristics and recognition efficiency of MIP were studied.•An off-line method for SDZ was developed using MIP as solid-phase extraction sorbent.•Seven seawater samples were determined from Jiaozhou Bay, Qingdao.•The SDZ concentrations in two seawater samples were 0.33 and 0.28 μg L−1.A highly selective sample cleanup procedure featuring molecularly imprinted solid-phase extraction (MISPE) was developed for the isolation and determination of sulfadiazine (SDZ) in seawater samples from Jiaozhou Bay, China. The molecularly imprinted polymer (MIP) was prepared using SDZ as the template molecule and methacrylic acid as the functional monomer. The MIP was used as a selective sorbent for the solid-phase extraction of SDZ. An off-line MISPE method followed by high-performance liquid chromatography (HPLC) with diode-array detection was established for the analysis of SDZ. Good linearity of the MISPE column for SDZ standard solutions was obtained within 0–200 μg L−1 (R2 > 0.99). The recoveries of spiked seawater samples were satisfactory as high as 88%. Finally, seven samples in Jiaozhou Bay were determined and there was no sulfadiazine found except #2 and #5 sample. The concentrations were respectively 0.33 μg L−1 and 0.28 μg L−1, and the relative standard deviations were 1.35% and 4.13% (n = 3).
Co-reporter:Ziru Lian
Journal of Separation Science 2013 Volume 36( Issue 5) pp:980-985
Publication Date(Web):
DOI:10.1002/jssc.201200939
A highly selective sample cleanup procedure combined with molecularly imprinted SPE was developed for the isolation of crystal violet from seawater and seafood samples. The molecularly imprinted polymer was prepared using crystal violet as the template molecule, methacrylic acid as the functional monomer, and ethylene glycol dimethacrylate as the cross-linker. The crystal violet-imprinted polymer was used as the selective sorbent for the SPE of crystal violet. An off-line molecularly imprinted SPE method followed by HPLC with diode-array detection for the analysis of crystal violet was also established. Good linearity on the molecularly imprinted SPE columns was obtained from 0 to 200 μg/L (R2 > 0.99). The result demonstrated that the proposed method can be used for the direct determination of crystal violet in seawater and seafood samples. Finally, five samples were analyzed and the following crystal violet concentrations were obtained: 0.92 and 0.52 μg/L in two seawater samples, as well as 0.36 and 0.27 μg/kg in two seafood samples. There is no crystal violet detected in the third seawater sample.
Co-reporter:Jiangtao Wang;Ziru Lian;Hedong Wang;Xiaoxiao Jin ;Yajuan Liu
Journal of Applied Polymer Science 2012 Volume 123( Issue 6) pp:3242-3247
Publication Date(Web):
DOI:10.1002/app.34997
Abstract
Chitosan, a biocompatible, biodegradable, nontoxic polymer, is prepared from chitin, which is the second most naturally occurring biopolymer after cellulose. Schiff base of chitosan, sorbyl chitosan, and p-aminobenzoyl chitosan were synthesized working under high-intensity ultrasound and their antimicrobial properties were analyzed against Escherichia coli, Staphylococcus aureus, and Aspergillus niger. The structures of the derivatives were characterized by FTIR spectroscopy and elemental analysis. The results of antimicrobial activities indicated that the antimicrobial activities of the derivatives increased with increasing the concentration. The antibacterial activity of schiff base of chitosan against E. coli was stronger, while acylated chitosan had better inhiting effect on S. aureus than others. It was also found that the antifungal activities of the derivatives were stronger than that of chitosan, and schiff base of chitosan was obviously superior to acylated chitosan. © 2011 Wiley Periodicals, Inc. J Appl Polym Sci, 2012
Co-reporter:Jiangtao Wang, Ji Hu, Shuwei Zhang
Journal of Colloid and Interface Science 2010 Volume 349(Issue 2) pp:578-582
Publication Date(Web):15 September 2010
DOI:10.1016/j.jcis.2010.04.081
The sorption of tetracycline onto clays and marine sediment from seawater was investigated at different pH levels, salinities (ionic strength), and temperatures. The sorption isotherms for tetracycline onto kaolinite, montmorillonite, and sediment clay were found to fit well with the Freundlich model. It is found that the linear model was better for marine sediment. Generally the sorption decreased with increasing pH and salinity. The individual sorption coefficient, Kdn, calculated from an empirical model, showed that the sorption coefficient of positive species, Kd+, was higher than that of zwitterions, Kd0, and negative species, Kd−, for montmorillonite, sediment clay, and marine sediment. The sorption capacity of marine sediment was lower than that of sediment clay due to the organic matter covered on the sorption sites. The results of the thermodynamic function calculation showed that the sorption was a spontaneous and exothermic process which was accompanied with a decrease in the degree of freedom.This study investigated the sorption of tetracycline onto clays and marine sediment, and developed an empirical model that calculated the individual sorption coefficient (Kdn) for each species of tetracycline.
Co-reporter:Jiangtao Wang, Xiaoxiao Jin, Defu Chang
Carbohydrate Polymers 2009 Volume 78(Issue 1) pp:175-177
Publication Date(Web):4 August 2009
DOI:10.1016/j.carbpol.2009.03.032
Chitosan (CTS), a biocompatible, biodegradable, nontoxic polymer, shows poor affinity for organic solvents. A novel chitosan derivative carrying the p-acetamidobenzoylate group was synthesized by the acylation reaction of chitosan with p-acetamidobenzoylate chloride in an acetic acid system under high-intensity ultrasound. The maximum substitution degree of the derivative was 0.42. The structure of the p-acetamidobenzoylate chitosan was characterized by FT-IR spectrometry, UV spectrometry and elemental analysis. The UV results showed that the derivative had good ultraviolet absorption at 273 nm. The solubility of the derivative was higher than that of chitosan. Taking advantage of the known capacity of solubility and ultraviolet absorption, the new derivative opens new possibilities for use as a sunscreen.
Co-reporter:Xiaoxiao Jin, Jiangtao Wang, Jie Bai
Carbohydrate Research 2009 Volume 344(Issue 6) pp:825-829
Publication Date(Web):21 April 2009
DOI:10.1016/j.carres.2009.01.022
Chitosan, a biocompatible, biodegradable, non-toxic polymer, is prepared from chitin, which is the second most naturally occurring biopolymer after cellulose. The Schiff base of chitosan was synthesized by the reaction of chitosan with citral working under high-intensity ultrasound. The effect of the molar ratio of chitosan to citral, reaction time, and temperature on the yield has been investigated. The optimal conditions were a temperature of 50 °C, a molar ratio of chitosan to citral of 1:6, and a reaction time of 10 h. The maximum yield achieved was 86.4% under optimum conditions. The structure of the Schiff base was characterized by FTIR spectroscopy, elemental analysis, and X-ray diffraction studies. The strong peaks at 1648.3 and 1610.6 cm−1 are due to CN and CC stretching vibrations. The results confirmed that amino groups on chitosan reacted with citral to form the Schiff base. The antimicrobial activities of chitosan and Schiff base of chitosan were investigated against Escherichia coil, Staphylococcus aureus, and Aspergillus niger. The results indicate that the antimicrobial activity of the Schiff base increases with an increase in the concentration. It was also found that the antimicrobial activity of the Schiff base was stronger than that of chitosan.
Co-reporter:Xingliang Song, Jinhua Li, Jiangtao Wang, Lingxin Chen
Talanta 2009 Volume 80(Issue 2) pp:694-702
Publication Date(Web):15 December 2009
DOI:10.1016/j.talanta.2009.07.051
Molecular imprinted polymers (MIPs) were prepared through thermal polymerization by using quercetin as the template molecule, acrylamide (AA) as the functional monomer and ethylene glycol dimethacrylate (EDMA) as the cross-linker in the porogen of tetrahydrofuran (THF). The synthesized MIPs were identified by both Fourier transform infrared (FTIR) and scanning electron microscope (SEM). Systematic investigations of the influences of key synthetic conditions, including functional monomers, porogens and cross-linkers, on the recognition properties of the MIPs were conducted. Scatchard analysis revealed that the homogeneous binding sites were formed in the polymers. Besides quercetin, two structurally similar compounds of rutin and catechol were employed for molecular recognition specificity tests of MIPs. It was observed that the MIPs exhibited the highest selective rebinding to quercetin. Accordingly, the MIPs were used as a solid-phase extraction (SPE) sorbent for the extraction and enrichment of quercetin in cacumen platycladi samples, followed by HPLC-UV analysis. The application of MIPs with high affinity and excellent stereo-selectivity toward quercetin in SPE might offer a novel method for the enrichment and determination of flavonoid compounds in the natural products.
Co-reporter:Ziru Lian, Jiangtao Wang
Marine Pollution Bulletin (15 October 2016) Volume 111(Issues 1–2) pp:411-417
Publication Date(Web):15 October 2016
DOI:10.1016/j.marpolbul.2016.07.034
•Ciprofloxacin molecularly imprinted polymers (Cip-MIPs) were prepared.•The characteristics and recognition efficiency of MIPs were studied.•An off-line method for Cip was developed using MIPs as solid-phase extraction.•Cip in five seawater samples from Jiaozhou Bay of China was determined.A high selective pre-treatment method for the cleanup and preconcentration of ciprofloxacin in natural seawater samples was developed based on molecularly imprinted solid-phase extraction (MISPE). The ciprofloxacin imprinted polymers were synthesized and the characteristics of obtained polymers were evaluated by scanning electron microscopy, Fourier transform infrared spectroscopy and binding experiments. The imprinted materials showed high adsorption ability for ciprofloxacin and were applied as special solid-phase extraction sorbents for selective separation of ciprofloxacin. An off-line MISPE procedure was optimized and the developed MISPE method allowed direct purification and enrichment of the ciprofloxacin from the aqueous samples prior to high-performance liquid chromatography analysis. The recoveries of spiked seawater on the MISPE cartridges ranged from 75.2 to 112.4% and the relative standard deviations were less than 4.46%. Five seawater samples from Jiaozhou Bay were analyzed and ciprofloxacin was detected in two samples with the concentrations of 0.24 and 0.38 μg L− 1, respectively.
Co-reporter:Haijiang Zhang, Chuanyuan Wang, Ruxiang Zhao, Xiaonan Yin, Hongyang Zhou, Liju Tan, Jiangtao Wang
Marine Pollution Bulletin (15 May 2016) Volume 106(Issues 1–2) pp:58-61
Publication Date(Web):15 May 2016
DOI:10.1016/j.marpolbul.2016.03.036
•The distributions of phenanthrene, anthracene, methyl-phenanthrene and methyl-anthracene in HFOs and crude oils were studied.•Nine new diagnostic ratios were developed for effective distinguishing HFOs from crude oils.•Bayes discriminant analysis demonstrated high precision with discriminant ratio lied between 93.92% and 99.32%.The heavy fuel oils (HFOs) and crude oils are the main oil types in the marine oil spill accidents in China. It is usually a challenge to distinguish the HFOs from crude oils due to the highly similar physicochemical characteristics. In this paper, the distributions of phenanthrene (Phe), anthracene (Ant), methyl-phenanthrene (MP) and methyl-anthracene (MA) in hundreds of HFOs and crude oils samples which were collected from all over the world were characterized. Nine new diagnostic indexes, such as Ant/(Ant + Phe) and other eight diagnostic ratios based on the MP isomers and MA, were developed for effective distinguishing HFOs from crude oils. The histogram with normal fit plots, the double ratio plots and Bayes discriminant analysis (BDA) method were employed to illustrate the effectiveness of the new diagnostic indexes. BDA model based on nine new diagnostic indexes demonstrated high precision with discriminant ratio which lay between 93.92% and 99.32%.
Co-reporter:Yuping Zhou, Liju Tan, Qiuting Pang, Feng Li, Jiangtao Wang
Marine Pollution Bulletin (15 June 2015) Volume 95(Issue 1) pp:107-114
Publication Date(Web):15 June 2015
DOI:10.1016/j.marpolbul.2015.04.034
•Ulva prolifera was grown on a mesocosm apparatus with three nutrient supply styles.•Ulva prolifera has higher P utilized rate during the whole culture period.•DIN supply content was essentially decisive for the output of U. prolifera.•The organic matter output of Ulva prolifera is begot according to DIN content.The influence of nutrients on the growth of Ulva prolifera was studied in the SYS by field experiments. The wet weight of U. prolifera gradiently increased from 11.94% to 25.92% in proportion to contents of DIN supply, which indicated DIN content was essentially decisive for the output of U. prolifera blooms. Continuous nutrient supply could promote the growth of U. prolifera, indicated by the increase of growth rate from 10.46% of the batch culture to 42.17% of the in situ culture. The higher P utilized rate in all treatments showed P was the potential limited factor for the growth of U. prolifera. Moreover, it was calculated about 4.1 × 105 t organic matter was begot by U. prolifera in the whole Yellow Sea based on the statistical relationship between output of U. prolifera and DIN content. This work could be convenient to evaluate biomass and prepare enough tools to manage U. prolifera.
Co-reporter:Xiuping He, Xiaoqi Mei, Jiangtao Wang, Ziru Lian, Liju Tan, Wei Wu
Marine Pollution Bulletin (15 January 2016) Volume 102(Issue 1) pp:142-147
Publication Date(Web):15 January 2016
DOI:10.1016/j.marpolbul.2015.11.041
•The diethylstilbestrol molecularly imprinted polymer microspheres (DES-MIPMs) were prepared.•The characteristics, binding capacity and recyclability of the MIPMs were studied.•An off-line method using MIPMs as solid phase extraction sorbent for DES was developed.•Four seawater samples were determined in two aquaculture areas.•The DES concentration in one sample was detected.An effective and highly selective molecularly imprinted material was prepared by suspension polymerization for the isolation and pre-concentration of synthetic estrogen diethylstilbestrol (DES) in seawater. The obtained MIPMs were proved to have more uniform size and porous structure, with maximum adsorption capacity of 8.43 mg g− 1 almost two times more than NIPMs (4.43 mg g− 1). The MIPMs showed no significant deterioration of the adsorption capacity after five rounds of regeneration. An off-line molecularly imprinted solid phase extraction (MISPE) method followed by HPLC–DAD was proposed for the detection of DES in seawater, and recoveries were satisfactorily higher than 77%. Four seawater samples in aquaculture area were analyzed and 0.61 ng mL− 1 DES was detected in one sample. The result demonstrated that this method can be used for the rapid separation and clean up of trace residual of DES in seawater.Download full-size image
Co-reporter:Ziru Lian, Zhenlin Liang, Jiangtao Wang
Marine Pollution Bulletin (15 February 2015) Volume 91(Issue 1) pp:96-101
Publication Date(Web):15 February 2015
DOI:10.1016/j.marpolbul.2014.12.022
•The mebendazole molecularly imprinted polymer (Meb-MIP) was prepared.•The characteristics and recognition efficiency of MIP were studied.•An off-line method for Meb was developed using MIP as solid-phase extraction.•The recovery of Meb from spiked seawater samples was determined.A high selective pre-treatment method for the extraction and analysis of mebendazole in environmental water samples was developed based on molecularly imprinted solid-phase extraction (MISPE). The mebendazole imprinted polymers were synthesized in acetonitrile using methacrylic acid and ethylene glycol dimethacrylate as functional monomer and cross-linker respectively. The imprinted materials showed high adsorption ability for mebendazole and were applied as special solid-phase extraction sorbents for selective separation of mebendazole. An off-line MISPE procedure was developed for the purification and enrichment of mebendazole from natural seawater samples prior to high-performance liquid chromatography analysis. The recoveries of spiked seawater on the MISPE cartridges were from 83.0% to 90.6%, and the values of the relative standard deviation were in the range of 2.78–4.13% (n = 3). The satisfied results showed that this pre-treatment methodology for extracting mebendazole in seawater was simple and effective.
