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Bing Zhao

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Organization: Institute of Process Engineering
Department: National Key Laboratory of Biochemical Engineering
Title:
In vitro antioxidant and antitumor activities of polysaccharides extracted from Asparagus officinalis
Co-reporter:Qingsheng Zhao, Bingxian Xie, Jun Yan, Fangchun Zhao, Jie Xiao, Lingyun Yao, Bing Zhao, Yunxiang Huang
Carbohydrate Polymers 2012 Volume 87(Issue 1) pp:392-396
Publication Date(Web):4 January 2012
DOI:10.1016/j.carbpol.2011.07.068
Ultrasonic circulating extraction technology was applied for the polysaccharide extraction from Asparagus officinalis. The crude polysaccharides were deproteinized by Sevag method and three main polysaccharide fractions, AOP-4, AOP-6 and AOP-8 were obtained by fractional precipitation with gradient concentrations of ethanol (40%, 60% and 80%). The in vitro antitumor and antioxidant activities of the polysaccharide fractions were evaluated by MTT assay and free radical-scavenging assay, respectively. Deproteinized AOPs showed higher antioxidant and antitumor activities than crude AOP. AOP-4 with molecular weight 5.75 × 104 Da showed significant function of scavenging hydroxyl radical. Three AOP fractions had significant antitumor activity against HeLa and BEL-7404 cells in a dose dependent manner. Furthermore, the inhibit activity of AOP-4 against HeLa cells was higher than those of other AOPs and the inhibition rate reached 83.96% at the concentration of 10 mg/mL. These results indicated that the AOP might be useful for developing natural safe antitumor drugs or health food.Highlights► Asparagus polysaccharide showed remarkable antioxidant and antitumor activities. ► AOP-4 with Mw 5.75 × 104 Da showed significant scavenging hydroxyl radical. ► Three AOP fractions had significant antitumor activity in a dose dependent manner.
Simultaneous analysis of diosgenin and sarsasapogenin in Asparagus officinalis byproduct by thin-layer chromatography
Co-reporter:Liwei Wang;Xiaodong Wang;Xiaofan Yuan
Phytochemical Analysis 2011 Volume 22( Issue 1) pp:14-17
Publication Date(Web):
DOI:10.1002/pca.1244

Abstract

Introduction – Asparagus officinalis L. has several biological activities including antifungal, antiviral and antitumoral activities due to the steroidal saponins. Normally diosgenin and sarsasapogenin are analysed separately by thin-layer chromatography or high-performance liquid chromatography (HPLC-UV or HPLC-ELSD), which is time-consuming and expensive, so we need to find a rapid solution to this problem.

Objective – To develop a sensitive, rapid and validated TLC method for simultaneous detection and quantification of diosgenin and sarsasapogenin.

Methodology – Samples were prepared by extraction of A. officinalis with 70% aqueous ethanol to get steroidal saponins, and then hydrolysed using 36 mL 2 m hydrochloric acid for 3 h. The hydrolysis product was extracted with chloroform, and then analysed by TLC, the results of which were verified by HPLC and HPLC-MS.

Results – The retention factor (Rf) of diosgenin and sarsasapogenin on TLC plate were 0.49 and 0.6, respectively. After calculation from the regression equation of the standard curve, the contents of diosgenin and sarsasapogenin in the A. officinalis extract were 0.27–0.46 and 0.11–0.32%, respectively.

Conclusion – The study showed that thin-layer chromatography can be applied for the determination of diosgenin and sarsasapogenin in the oldest tissue of A. officinalis, and also can be conducted for screening of sapogenin in other plant or extracts. Copyright © 2010 John Wiley & Sons, Ltd.

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