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STEPHEN BUTLER

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Organization: Loughborough University , England
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Title: Lecture(PhD)
A continuous luminescence assay for monitoring kinase activity: signalling the ADP/ATP ratio using a discrete europium complex
Co-reporter:Sarah H. Hewitt;Jonathan Parris;Romain Mailhot;Stephen J. Butler
Chemical Communications 2017 vol. 53(Issue 94) pp:12626-12629
Publication Date(Web):2017/11/23
DOI:10.1039/C7CC05887J
We report the application of a stable cationic europium complex [Eu.1]+ in a continuous-read luminescence assay for kinase activity. [Eu.1]+ binds reversibly to ATP and ADP in water, at neutral pH, in the presence of Mg2+ ions, providing distinctive luminescence responses that permits the kinase-catalysed conversion of ATP to ADP to be monitored in real-time.
1,7-Bis(tert-butoxycarbonylmethyl)-1,4,7,10-tetraazacyclododecane
1,7-Bis(tert-butoxycarbonylmethyl)-1,4,7,10-tetraazacyclododecane
CAS:162148-48-3
1,7-Bis(tert-butoxycarbonylmethyl)-1,4,7,10-tetraazacyclododecane