Douglas Young - Cochemist

Chemicals
References

Synthesis of homochiral tetrahydropteridines

Co-reporter:Stephen J. Baker, Kenneth J.M. Beresford, Douglas W. Young

Tetrahedron 2014 70(40) pp: 7221-7228

Publication Date(Web):

DOI:10.1016/j.tet.2014.06.048

Synthesis, Structural and Biological Studies of Ubiquitin Mutants Containing (2S, 4S)-5-Fluoroleucine Residues Strategically Placed in the Hydrophobic Core

Co-reporter:Dmitriy Alexeev Dr.;Paul N. Barlow Dr.;Stella M. Bury Dr.;Jean-Damien Charrier Dr.;Alan Cooper Dr.;David Hadfield Dr.;Craig Jamieson Dr.;Sharon M. Kelly Dr.;Robert Layfield Dr.;R. John Mayer Dr.;Helen McSparron Dr.;Nicholas C. Price Dr.;Robert Ramage Dr.;Lindsay Sawyer Dr.;Bernard A. Starkmann Dr.;Dusan Uhrin Dr.;Jill Wilken Dr.;Douglas W. Young Dr.

ChemBioChem 2003 Volume 4(Issue 9) pp:

Publication Date(Web):4 SEP 2003

DOI:10.1002/cbic.200300699

Insider report: A fluoroleucine mutant of ubiquitin (cyan) with two strategically placed fluorine reporter groups in its core was synthesised. ¹H NMR, crystallography, CD spectroscopy, biological assay and calorimetry indicate minimal structural and functional perturbation compared to the native protein (yellow; see picture). ¹⁹F NMR spectra indicate changes within the core during folding and unfolding.

Stereochemistry of the reaction of the inhibitor β-chloroalanine with mercaptoethanol, a β-substitution reaction catalysed by an aminotransferase

Co-reporter:Benjamin Adams, Kenneth J. M. Beresford, Sheena M. Whyte and Douglas W Young

Chemical Communications 2000 (Issue 7) pp:619-620

Publication Date(Web):22 Mar 2000

DOI:10.1039/B000442L

L-Aspartate aminotransferase, a member of the α-family of PLP mediated enzymes, which normally catalyses transamination, has been used to catalyse the β-substitution reaction of stereospecifically labelled samples of the enzyme inhibitor β-chloro-L-alanine with 2-mercaptoethanol; the stereochemistry of the products was assigned by independent synthesis, showing that the abnormal substitution reaction proceeds with overall retention of stereochemistry, the usual stereochemical consequence of reactions catalysed by enzymes of the β-family of PLP mediated enzymes which have low homology with enzymes of the α-family.