Jian Shen

Find an error

Name: 沈健

Organization: Nanjing Normal University , China

Department: Jiangsu Engineering Research Center for Biomedical Function Materials

Title: Professor(PhD)

Chemicals
References

In Situ Growth of Mesoporous Silica with Drugs on Titanium Surface and Its Biomedical Applications

Co-reporter:Mimi Wan, Jin Zhang, Qi Wang, Shuyue Zhan, Xudong Chen, Chun Mao, Yuhong Liu, and Jian Shen

ACS Applied Materials & Interfaces June 7, 2017 Volume 9(Issue 22) pp:18609-18609

Publication Date(Web):May 17, 2017

DOI:10.1021/acsami.7b05163

Mesoporous silica has been developed for the modification of titanium surfaces that are used as implant materials. Yet, the traditional modification methods failed to effectively construct mesoporous silica on the titanium surface evenly and firmly, in which the interaction between mesoporous silica and titanium was mainly physical. Here, in situ growth of mesoporous silica on a titanium surface was performed using a simple evaporation-induced self-assembly strategy. Meantime, in situ introduction of drugs (heparin and vancomycin) to mesoporous silica was also adopted to improve the drug-loading amount. Both the above-mentioned processes were completed at the same time. Transmission electron microscopy, N2 adsorption–desorption isotherms, Fourier transform infrared spectroscopy, scanning electron microscopy, and water contact angle measurements were used to characterize the structure of the mesoporous silica film. Results indicated that the mesoporous silica film that in situ grew on the titanium surface was smooth, thin, transparent, and stable. Cytotoxicity, proliferation performance of osteoblast cells, and in vitro and in vivo studies of the antibacterial activity of the coating were tested. This is the first study to modify the titanium surface by the in situ growth of a mesoporous silica coating with two kinds of drugs. The stability of the mesoporous silica coating can be attributed to the chemical bonding between dopamine and silicon hydroxyl of the mesoporous silica coating, and the smooth surface of mesoporous silica is a result of the method of in situ growth. The large amount of drug-loading also could be ascribed to the in situ introduction of drugs during the synthetic process. The strategy proposed in this work will bring more possibilities for the preparation of advanced functional materials based on the combination of mesoporous structure and metallic materials.Keywords: biomedical applications; drug delivery; in situ growth; mesoporous silica coatings; titanium surface;

Electrospun polyurethane/keratin/AgNP biocomposite mats for biocompatible and antibacterial wound dressings

Co-reporter:Yanfang Wang, Pengfei Li, Ping Xiang, Jueting Lu, Jiang Yuan and Jian Shen

Journal of Materials Chemistry A 2016 vol. 4(Issue 4) pp:635-648

Publication Date(Web):21 Dec 2015

DOI:10.1039/C5TB02358K

Keratin based biomaterials have emerged as potential candidates for various biomedical and biotechnological applications due to their intrinsic biocompatibility, biodegradability, mechanical durability, and natural abundance. The objective of this study is to combine the merits of polyurethane, keratin, and silver nanoparticles (AgNPs) together and develop a novel nanofibrous mat for wound dressing. Herein, keratin was first extracted from human hair and chemically modified with iodoacetic acid to afford S-(carboxymethyl) keratin. The modified keratin was examined using Raman spectroscopy, infrared spectroscopy, and SDS-PAGE. The keratin was then blended with polyurethane (PU) and electrospun. Subsequently, AgNPs were formed in situ to afford antibacterial PU/keratin/AgNP mats. These mats were characterized using field emission scanning electron microscopy (FE-SEM), attenuated total reflection Fourier transform infrared spectroscopy (ATR-FTIR), water contact angle measurements, and X-ray photoelectron spectroscopy (XPS). MTT results indicated that the introduction of keratin could accelerate fibroblast cell proliferation, while the loaded AgNPs did not weaken cytocompatibility. Antibacterial test results showed that PU/keratin/AgNP mats exerted good antibacterial property. The results from a wound healing test and a histological examination suggested that these biocomposite mats could remarkably accelerate wound recovery as compared to the conventional gauze sponge dressing. Given their excellent biocompatibility, antibacterial properties, and very mild inflammatory responses, PU/keratin/AgNP mats have great potential for wound dressing applications.

Extraction, characterization, and NO release potential of keratin from human hair

Co-reporter:Xingxing Jin, Yanfang Wang, Jiang Yuan, Jian Shen

Materials Letters 2016 Volume 175() pp:188-190

Publication Date(Web):15 July 2016

DOI:10.1016/j.matlet.2016.04.036

•Keratin is extracted and modified to afford S-carboxymethyl keratin.•The amino acid residues, isoelectric point, and molecular weight are quantified.•Keratin is capable of promoting NO release from GSNO in the presence of glutathione.Keratin-based biomaterials have emerged as potential candidates for various biomedical and biotechnological applications due to their intrinsic biocompatibility, biodegradability, mechanical durability, and natural abundance. The objective of our study is to explore the feasibility and potential applications of keratin for promoting nitric oxide (NO) release. Herein, keratin was first extracted from human hair and then modified with iodoacetic acid to afford S-carboxymethyl keratin. The amino acid residues, isoelectric point, and molecular weight of keratin were quantified with amino acid analyzer, zeta potential measurement, and SDS-PAGE method. Interestingly, human hair keratin was proved to be capable of promoting NO release from endogenous donor of S-nitrosoglutathione (GSNO) in the presence of glutathione.Keratin was extracted and modified to afford S-carboxymethyl keratin. The amino acid residues, isoelectric point, and molecular weight of keratin were quantiﬁed with amino acid analyzer, zeta potential measurement, and SDS-PAGE method. Human hair keratin was proved to be capable of promoting NO release from endogenous donor of S-nitrosoglutathione (GSNO) in the presence of glutathione (GSH).

Differences in cytocompatibility between collagen, gelatin and keratin

Co-reporter:Yanfang Wang, Weiwei Zhang, Jiang Yuan, Jian Shen

Materials Science and Engineering: C 2016 Volume 59() pp:30-34

Publication Date(Web):1 February 2016

DOI:10.1016/j.msec.2015.09.093

•Collagen, gelatin and keratin were coelectrospun with PHBV to afford nanofibrous mats.•Cytocompatibility was evaluated with cell adhesion, cell viability and cell proliferation.•Collagen had significantly superior cytocompatibility as compared to gelatin and keratin.Keratins are cysteine-rich intermediate filament proteins found in the cytoskeleton of the epithelial cells and in the matrix of hair, feathers, wool, nails and horns. The natural abundance of cell adhesion sequences, RGD (Arg-Gly-Asp) and LDV (Leu-Asp-Val), makes them suitable for tissue engineering applications. The purpose of our study is to evaluate their cytocompatibility as compared to well-known collagen and gelatin proteins. Herein, collagen, gelatin and keratin were blended with poly(hydroxybutyrate-co-hydroxyvalerate) (PHBV) and electrospun to afford nanofibrous mats, respectively. These PHBV/protein composite mats were characterized by field emission scanning electron microscopy (FE-SEM), attenuated total reflection Fourier transform infrared spectroscopy (ATR-FTIR), X-ray photoelectron spectroscopy (XPS), and dynamic mechanical analysis (DMA). The cytocompatibility was evaluated with cell adhesion, cell viability and cell proliferation. The data from MTT and BrDU revealed that collagen had significantly superior cytocompatibility as compared to gelatin and keratin. Gelatin showed a better cytocompatibility than keratin without statistical significance difference. Finally, we gave the reasons to account for the above conclusions.

Zwitterionic polymer brushes via dopamine-initiated ATRP from PET sheets for improving hemocompatible and antifouling properties

Co-reporter:Xingxing Jin, Jiang Yuan, Jian Shen

Colloids and Surfaces B: Biointerfaces 2016 Volume 145() pp:275-284

Publication Date(Web):1 September 2016

DOI:10.1016/j.colsurfb.2016.05.010

•Polyzwitterions along with weak cationic PDMAEMA surfaces are formed via dopamine-initiated ATRP.•The grafted PET sheets show outstanding hemocompatibility and anti-biofouling property.•Polyzwitterions along with weak cationic PDMAEMA surfaces are ideal for antifouling and antibacterial usage.A low-fouling zwitterionic surface strategy has been proven to be promising and effective for repelling nonspecific adsorption of proteins, cells and bacteria, which may eventually induce adverse pathogenic problems such as thrombosis and infection. Herein, a multi-step process was developed by a combination of mussel-inspired chemistry and surface-initiated atom transfer radical polymerization (SI-ATRP) technique for improving hemocompatible and anti-biofouling properties. Polyethylene terephthalate (PET) sheets were first treated with dopamine, and then the bromoalkyl initiators were immobilized on the poly(dopamine) functionalized surfaces, followed by surface-initiated activators regenerated by electron transfer atom transfer radical polymerization (ARGET ATRP) of 2-(dimethylamino) ethyl methacrylate (DMAEMA) monomer. Subsequently, the resulting PET sheets were ring-opening reacted with 1,3-propiolactone (PL) and 1,3-propanesultone (PS) to afford polycarboxybetaine and polysulfobetaine brushes, respectively. Characterizations of the PET sheets were undertaken by attenuated total reflection Fourier transform infrared spectroscopy (ATR-FTIR), atomic force microscope (AFM), water contact angle (WCA) measurements, and X-ray photoelectron spectroscopy (XPS) analysis, respectively. The conversion rates of PDMAEMA to polyzwitterions were evaluated by XPS analysis. The remained PDMAEMA(weak cationic) and formed zwitterions(neutral) would form a synergetic antifouling and antibacterial surface. Hemocompatible and anti-biofouling properties were evaluated by total adsorption of protein as well as the adhesion of platelet, cell and bacterium. Zwitterionic polymer brushes grafted PET sheets showed outstanding hemocompatibility featured on reduced platelet adhesion and repelled protein adsorption. Meanwhile, the grafted PET sheets exerted excellent anti-biofouling property characterized by the resisted adhesion of Escherichia coli and 3T3 cells. In summary, zwitterionic polymer brushed modified PET sheets have a great potential for biomedical applications.

Preparation of keratin/chlorhexidine complex nanoparticles for long-term and dual stimuli-responsive release

Co-reporter:Xuelian Zhi, Yanfang Wang, Pengfei Li, Jiang Yuan and Jian Shen

RSC Advances 2015 vol. 5(Issue 100) pp:82334-82341

Publication Date(Web):16 Sep 2015

DOI:10.1039/C5RA16253J

Nanoscale polyion complex formation via the electrostatic complexation of a polyelectrolyte and a charged drug is the most convenient method for building a drug delivery system that simultaneously realizes the carrier preparation and drug embedding. Herein, we prepared keratin/chlorhexidine complex nanoparticles (KCNPs) based on a drug-induced ionic gelation technique without using a crosslinker, organic solvent or surfactant. These KCNPs exhibited good stability even after having been long-standing for 14 days. The KCNPs were characterized using FTIR, DLS, SEM and TEM. It was found that these nanoparticles had a spherical morphology with a diameter of about 180 nm, and a negatively charged surface with a zeta potential of about −39.1 mV. The cell toxicity of the KCNPs at different dosage levels was evaluated using the MTT assay method, indicating their slight cytotoxicity at lower dosages. The antibacterial activity against E. coli and S. aureus was determined using the zone of inhibition method. It seemed that the KCNPs had better antibacterial activity against S. aureus than against E. coli. Drug delivery profiles showed that the chlorhexidine (CHX) loaded nanoparticles exhibited both pH- and glutathione-responsive character. These keratin-based complex nanoparticles can be regarded as a valuable stimuli-responsive strategy for the delivery of anticancer agents.

Anti-biofouling ability and cytocompatibility of the zwitterionic brushes-modified cellulose membrane

Co-reporter:Pingsheng Liu, Qiang Chen, Li Li, Sicong Lin and Jian Shen

Journal of Materials Chemistry A 2014 vol. 2(Issue 41) pp:7222-7231

Publication Date(Web):29 Aug 2014

DOI:10.1039/C4TB01151A

Blood compatibility and cytocompatibility are key requirements of blood-contacting biomaterials for biomedical applications. Here, we showed the general zwitterionic surface modification of cellulose membrane (CM) substrates, via surface-initiated atom transfer radical polymerization (SI-ATRP), to enhance the anti-biofouling ability without compromising the cytocompatibility of the substrates. Robust X-ray photoelectron spectroscopy (XPS) characterization revealed the successful construction of three zwitterionic brushes, which significantly increased the hydrophilicity of the CM substrates. The zwitterionic brushes-modified CM substrates can significantly reduce the non-specific adsorption of proteins, platelet adhesion and cell attachment, indicating a generally and significantly improved anti-biofouling ability, which is comparable to that of the benchmark anti-fouling poly(ethylene glycol) (PEG) surface. Inspired by the varied in situ cell morphology observed on different substrates, we further investigated the cytocompatibility of the zwitterionic coatings and showed the general cytocompatibility of zwitterionic brush-modified CM surfaces based on both 2D (cell cultured with zwitterionic surfaces) and 3D cell cultures (cell encapsulation in zwitterionic hydrogels). This work provides a promising general approach to enhancing the blood compatibility of cellulose-based materials without compromising their cytocompatibility. The cytocompatibility observation may enrich the perceptions of the zwitterionic modification of substrates and may be beneficial for the in vivo applications of zwitterionic materials.

A chitosan-Au-hyperbranched polyester nanoparticles-based antifouling immunosensor for sensitive detection of carcinoembryonic antigen

Co-reporter:Chong Sun, Lie Ma, Qiuhui Qian, Soniya Parmar, Wenbo Zhao, Bo Zhao and Jian Shen

Analyst 2014 vol. 139(Issue 17) pp:4216-4222

Publication Date(Web):22 Apr 2014

DOI:10.1039/C4AN00479E

Analysts are always interested in finding new functional nanomaterials and devices with good properties for electrochemical sensor applications. In this paper, hyperbranched polyester nanoparticles with carboxylic acid functional groups (HBPE–CA NPs) were synthesized and combined with chitosan wrapped around Au nanoparticles (CS–Au NPs) to prepare a novel and sensitive electrochemical immunosensor by adsorption of carcinoembryonic antibody (anti-CEA) on the (HBPE–CA)/CS–Au NPs modified glass carbon electrode (GCE). Under the optimized conditions, the proposed immunosensor displayed a good amperometric response to carcinoembryonic antigen (CEA). Moreover, based on the antibiofouling properties, the immunosensor could be used for the direct detection of CEA in whole blood, and exhibited a wide detection range (1–107 fg mL−1), and a low detection limit of 0.251 fg mL−1 (signal/noise = 3). Control experiments were also carried out by using ascorbic acid (AA), uric acid (UA), human immunoglobulin G (IgG), BSA and glucose in the absence of CEA. The good stability and repeatability of this immunosensor were also proven. Importantly, the results of the detection of clinical whole blood specimens with the proposed immunosensor showed good consistency with the data determined by enzyme-linked immunosorbent assay (ELISA) in serum samples. Furthermore, the developed immunosensor could provide a promising immunoassay strategy for clinical applications, since the values we measured in whole blood directly are likely closer to the real values.

Electrochemical immunosensor based on hyperbranched structure for carcinoembryonic antigen detection

Co-reporter:Jingjing Miao, Xiaobo Wang, Liandi Lu, Peiyuan Zhu, Chun Mao, Haolin Zhao, Youchao Song, Jian Shen

Biosensors and Bioelectronics 2014 Volume 58() pp:9-16

Publication Date(Web):15 August 2014

DOI:10.1016/j.bios.2014.02.024

•Novel immunsensor based on functionalized hyperbranched polyester was prepared.•The immunosensor showed a linear range from 0.01 to 80 ng/mL CEA.•The immunosensor showed a low detection limit of 2.36 pg/mL CEA.•The designed immunoassay showed ultrahigh sensitivity, efficient and economical.Sensitive determination of carcinoembryonic antigen (CEA) is very important in clinical research and diagnosis. Herein we report the design and synthesis of a new kind of immunosensor based on the benefits of hyperbranched structure. The hyperbranched polyester was grafted to the surface of indium tin oxides glass (ITO) electrode, and the grafting processes were characterized by attentuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR), X-ray photoelectron spectroscopy (XPS) and atomic force microscopy (AFM). After CEA and horse radish peroxidase (HRP)-labeled antibody-conjugated AuNPs (HRP–Ab2–AuNPs) bioconjugates were immobilized on the surface of the hyperbranched structure-modified electrode, the optimized conditions of the above electrode were investigated. Moreover, the analytical performance of the proposed immunosensor showed a high sensitivity, a linear range from 0.01 to 80 ng/mL with a low detection limit of 2.36 pg/mL, and good selectivity for CEA. The designed immunoassay system holds great potential for ultrasensitive electrochemical biosensing of other analytes.

Blood compatibility of a new zwitterionic bare metal stent with hyperbranched polymer brushes

Co-reporter:Xiaobo Wang, Xiaoqiang Chen, Lei Xing, Chun Mao, Hongxing Yu and Jian Shen

Journal of Materials Chemistry A 2013 vol. 1(Issue 38) pp:5036-5044

Publication Date(Web):31 Jul 2013

DOI:10.1039/C3TB20855A

Different approaches have been used in developing new coronary stents to protect against thrombosis and minimize restenosis. Here we describe a new zwitterionic bare metal stent (BMS) surface based on 2-((bromobutyryl)oxy)ethyl acrylate (BBEA) hyperbranched polymer. The new BMS produced was studied for its blood compatibility. The hemocompatibility studied including platelet adhesion tests, hemolysis assay, morphological changes of red blood cells (RBCs), coagulation time tests, plasma recalcification time (PRT) assay, complement activation, and platelet activation at the molecular level. Moreover the cytotoxicity of BMS before and after modification was also characterized by MTT assays. Most assays had remarkable differences in the presence of the new zwitterionic BMS, indicating the importance of the zwitterion.

Preparation of a novel immunosensor for tumor biomarker detection based on ATRP technique

Co-reporter:Xiaobo Wang, Min Zhou, Yinyan Zhu, Jingjing Miao, Chun Mao and Jian Shen

Journal of Materials Chemistry A 2013 vol. 1(Issue 16) pp:2132-2138

Publication Date(Web):13 Feb 2013

DOI:10.1039/C3TB00003F

A novel electrochemical immunosensor for sensitive detection of a tumor marker is reported. This protocol involves the surface-initiated atom transfer radical polymerization (SI-ATRP) of poly(ethylene glycol) monomethacrylate (PEGMA) on an indium tin oxide (ITO) electrode. Using carcinoembryonic antigen (CEA) as a model analyte, capture anti-CEA (Ab1) was conjugated to poly(poly(ethylene glycol) monomethacrylate) (P(PEGMA)) brushes, via activation of hydroxyl groups by succinic anhydride and N-(3-dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride, and N-hydroxysulfosuccinimide sodium salt (NHS/EDC). After the sandwich immunoreaction, the horseradish peroxidase-labeled signal anti-CEA antibodies were used to conjugate gold nanoparticles (HRP-Ab2–AuNPs bioconjugates). Under optimized conditions, the proposed immunosensor showed a high sensitivity and a linear range from 5 × 10−2 to 20 ng mL−1 with a low detection limit of 1.09 × 10−3 ng mL−1. The assay results of clinical serum samples were in acceptable agreement with the reference values. The designed immunoassay system with ultrahigh sensitivity and being efficient and economical, might have potentially broad applications in protein diagnostics and bioassay.

Preparation, blood compatibility and anticoagulant effect of heparin-loaded polyurethane microspheres

Co-reporter:Fengyu Tong, Xiaoqiang Chen, Libin Chen, Peiyuan Zhu, Jianfeng Luan, Chun Mao, Jianchun Bao and Jian Shen

Journal of Materials Chemistry A 2013 vol. 1(Issue 4) pp:447-453

Publication Date(Web):05 Nov 2012

DOI:10.1039/C2TB00250G

Recent advances in micro- and nanotechnology have provided a variety of particles with highly controlled shapes, sizes, chemical composition, and interesting properties. In this work, a novel kind of heparin-functionalized polyurethane microsphere (Hep-PU MS) was synthesized by a single-step phase separation method. The blood compatibility and anticoagulant effect of the Hep-PU MSs were investigated using coagulation tests, hemolysis assay, complement and platelet activation detection, cytotoxicity analysis, and drug loading and release study. The results confirmed that the heparin can substantially enhance the anticoagulant properties of PU MSs, and the Hep-PU MSs have the potential to be used as a mild anticoagulant compared to heparin. With the simplicity of the functionalized method, the excellent hemocompatibility and the slow-release of heparin, the Hep-PU MSs with desirable bioproperties can be readily tailored to cater to various biomedical applications.

Surface-initiated RAFT polymerization of sulfobetaine from cellulose membranes to improve hemocompatibility and antibiofouling property

Co-reporter:Jiang Yuan, Xiaobo Huang, Pengfei Li, Li Li and Jian Shen

Polymer Chemistry 2013 vol. 4(Issue 19) pp:5074-5085

Publication Date(Web):24 Jun 2013

DOI:10.1039/C3PY00565H

Two major complications generally occur in blood-contacting devices, namely thrombus formation and microbial invasion and infection. Therefore, hemocompatible and antibiofouling surfaces, which function as barriers to bacterial and cell adhesion, are essential. Herein, we report the successful grafting of zwitterionic polysulfobetaine brushes onto a cellulose membrane (CM) via surface-initiated reversible addition–fragmentation chain-transfer (SI-RAFT) polymerization for improving hemocompatibility and antibiofouling property. Both pristine and polysulfobetaine brush-modified CM substrates were characterized by attenuated total reflection Fourier transform infrared spectroscopy (ATR-FTIR), water contact angle measurements (WCA), X-ray photoelectron spectroscopy analysis (XPS), and atomic force microscopy (AFM). Experimental observations demonstrated the successful grafting of polysulfobetaine brushes, where brush thicknesses were found to increase gradually with polymerization time and monomer concentrations. Tests conducted by investigating platelet adhesion, hemolytic rates and protein adsorption indicated that polysulfobetaine brush-grafted CMs had excellent hemocompatibility featuring lower platelet adhesion and protein adsorption properties without causing hemolysis. E. coli adhesion and HeLa cell adhesion tests showed that grafted CMs had superior antibacterial adhesion properties and long-term cell adhesion resistance for up to four days. The functionalized cellulose substrate described holds great potential for use in biomedical applications.

Fabrication of glucose biosensor for whole blood based on Au/hyperbranched polyester nanoparticles multilayers by antibiofouling and self-assembly technique

Co-reporter:Chong Sun, Xiaohan Chen, Qiaorong Han, Min Zhou, Chun Mao, Qinshu Zhu, Jian Shen

Analytica Chimica Acta 2013 Volume 776() pp:17-23

Publication Date(Web):7 May 2013

DOI:10.1016/j.aca.2013.03.032

Acknowledging the benefits of hyperbranched polymers and their nanoparticles, herein we report the design and synthesis of sulfonic acid group functionalized hydroxyl-terminated hyperbranched polyester (H30-SO3H) nanoparticles and their biomedical application. The H30-SO3H nanoparticles were characterized by transmission electron microscopy (TEM), Fourier transform infrared (FTIR) spectroscopy and proton nuclear magnetic resonance spectroscopy (1H NMR). The good hemocompatibility of H30-SO3H nanoparticles was also investigated by coagulation tests, complement activation and platelet activation. The novel glucose biosensor was fabricated by immobilizing the positively charged Au nanoparticles, H30-SO3H nanoparticles and glucose oxidase (GOx) onto the surface of glassy carbon electrode (GCE). It can be applied in whole blood directly, which was based on the good hemocompatibility and antibiofouling property of H30-SO3H nanoparticles. The biosensor had good electrocatalytic activity toward glucose with a wide linear range (0.2–20 mM), a low detection limit 1.2 × 10−5 M in whole blood and good anti-interference property. The development of materials science will offer a novel platform for application to substance detection in whole blood.Graphical abstractHighlight•A novel method for detection of glucose in whole blood has been developed.•The method based on antibiofouling and self-assembly technology was investigated.•The antibiofouling technique utilized for sensor is significant for diagnostics.

Terbinafine hydrochloride intercalated in montmorillonite: synthesis and characterization

Co-reporter:Na Meng;Ning-Lin Zhou

Research on Chemical Intermediates 2013 Volume 39( Issue 2) pp:671-680

Publication Date(Web):2013 February

DOI:10.1007/s11164-012-0588-8

The novel antifungal hybrid of terbinafine hydrochloride (TER-HCl)/montmorillonite was synthesized by the intercalation method under mechanical stirring. Intercalation of TER-HCl in the MMT galleries was characterized by X-ray diffraction (XRD), Fourier transform infrared spectra, elemental analysis (EA). and thermogravimetric analysis (TGA). The results from IR, TGA, and EA showed a difference in chemical composition of the MMT and the TER-HCl/MMT. XRD analysis showed that the basal spacing of montmorillonite significantly expanded from 1.53 to 2.79 nm. TER-HCl was successfully intercalated into the interlayer of MMT, and 28 % of TER-HCl was released after 48 h in 0.9 % (w/v) NaCl aqueous solution (pH 7) at 37 ± 0.5 °C. The antifungal activity of the hybrid against Candida albicans was evaluated using the inhibitory zone method and the minimum inhibitory concentration. The TER-HCl/MMT strongly inhibited C. albicans. These results show that TER-HCl/MMT can be useful in biomedical applications.

Synthesis and one-pot tethering of hydroxyl-capped phosphorylcholine onto cellulose membrane for improving hemocompatibility and antibiofouling property

Co-reporter:Jiang Yuan, Ling Tong, Huaixiang Yi, Bingxiang Wang, Jian Shen, Sicong Lin

Colloids and Surfaces B: Biointerfaces 2013 Volume 111() pp:432-438

Publication Date(Web):1 November 2013

DOI:10.1016/j.colsurfb.2013.06.009

•Hydroxyl-capped phosphorylcholines with different carbon spacer lengths were synthesized.•HOPC was tethered onto cellulose surface with HDI in inverse PC form (CP form).•HOPC grafted cellulose exhibited good hemocompatibility and antifouling property.Tethering of biomimetic phosphorylcholine derivative onto the surface of biomedical devices is an effective method for improving hemocompatibility and antibiofouling property. Herein, series of novel hydroxyl-capped phosphorylcholines (HOPC) with different carbon spacer lengths were first synthesized and characterized with element analysis (EA), Fourier transform infrared spectroscopy(FTIR), and nuclear magnetic resonance spectroscopy (NMR). Then, HOPC (n = 5, 2a) was one-pot tethered onto cellulose membrane with hexamethylene diisocyanate (HDI) as a coupling agent. The existence of phosphorylcholine was demonstrated by water contact angle measurement, attenuated total reflection Fourier transform infrared spectroscopy (ATR-FTIR), atomic force microscopy (AFM), and X-ray photoelectron spectroscopy (XPS). The hemocompatibility and antibiofouling property were evaluated by hemolytic test, platelet adhesion, protein adsorption, and Escherichia coli adhesion test. The results showed that cellulose membranes tethered with HOPC exhibited excellent hemocompatibility featured by low platelet adhesion and ﬁbrinogen adsorption as well as antibiofouling property with bacterial adhesion resistance.

Hemocompatibility and anti-biofouling property improvement of poly(ethylene terephthalate) via self-polymerization of dopamine and covalent graft of zwitterionic cysteine

Co-reporter:Pengfei Li, Xianmei Cai, Ding Wang, Shuangchun Chen, Jiang Yuan, Li Li, Jian Shen

Colloids and Surfaces B: Biointerfaces 2013 110() pp: 327-332

Publication Date(Web):

DOI:10.1016/j.colsurfb.2013.04.044

Structureproperty investigations with dielectric study on phosphorylcholine-based polyurethane

Co-reporter:Qi Zhang;Dan Zhu;Fu Su;Yun Xie;Zhenmao Ma

Journal of Biomedical Materials Research Part A 2012 Volume 100A( Issue 7) pp:1868-1876

Publication Date(Web):

DOI:10.1002/jbm.a.34154

Abstract

Polyurethane with zwitterionic phosphorylcholine on the main chain was synthesized and the structures were defined with FTIR and ¹HNMR. The mechanical (tensile strength, elastic modulus) and biological (platelet adhesion) evaluations of its blend films with polyurethane were of satisfactory results, which were in accordance with the requirements of the medical devices, showing their potential applications as anticoagulant biomaterials. The dielectric spectroscopy was recorded with solid films and with films in water. The dielectric dispersion of the solid films demonstrated the existence of condensed ionic structures, which lead to the rigidity enhancement of the soft segment of the phosphorylcholine-based polyurethane, so that its elastic modulus increased. The dielectric measurement with films in water, providing a measurement for the surface properties in the aqueous environment, offered a semiquantitative description of the interface dynamics of the material with a double-layer model, based on which a new hypothesis on the mechanism of blood or bio-compatibility was proposed that the hydrated surface of the satisfactory biomaterials can response to the outside electromagnetic stimuli with slight strength and prompt relaxation. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2012.

Correlation between Dielectric/Electric Properties and Cross-Linking/Charge Density Distributions of Thermally Sensitive Spherical PNIPAM Microgels

Co-reporter:Jianfeng Zhou, Jingjing Wei, To Ngai, Li Wang, Dan Zhu, and Jian Shen

Macromolecules 2012 Volume 45(Issue 15) pp:6158-6167

Publication Date(Web):July 19, 2012

DOI:10.1021/ma300454h

Thermally sensitive poly(N-isopropylacrylamide) (PNIPAM) spherical microgels with different charge distribution were prepared by different monomer and comonomer feeding methods. The frequency (f, 10–2–107 Hz) and temperature (T, 5–50 °C) dependent complex dielectric/electric properties of these microgels were analyzed by dielectric relaxation spectroscopy. Each microgel can be treated as a large multicharged macroion surrounded by many small counterions, and the relative displacement and the diffusion of counterions contribute to the permittivity and conductivity of the microgel dispersion. Both the dielectric permittivity at f > 103 Hz and the conductivity at f > 106 Hz decrease when the microgels shrink at temperatures higher than the lower critical solution temperature (LCST) of PNIPAM due to the shrink-induced counterion dissociation. The variation of either permittivity or conductivity with temperature reveals a sharper transition for the microgels or the microgel shell with a more uniform charge distribution than those with an inhomogeneous dense core–loose shell structure, indicating a more gradual chain shrinkage for the latter. By detecting the counterions’ behaviors, we can use dielectric relaxation spectroscopy to probe the microscopic structural and dynamic heterogeneities of PNIPAM microgel dispersions.

Novel polyurethane ionomer nanoparticles displayed a good biosensor effection

Co-reporter:Wenbo Zhao, Guohui Zhang, Lancao Jiang, Tianhong Lu, Xiaohua Huang, Jian Shen

Colloids and Surfaces B: Biointerfaces 2011 Volume 88(Issue 1) pp:78-84

Publication Date(Web):1 November 2011

DOI:10.1016/j.colsurfb.2011.06.011

This study described the bioelectrochemistry property of hemoglobin (Hb) on biopolymer film of polyurethane ionomer nanoparticles (PUI-NPs) noncovalently functionalized with multiwall carbon nanotubes (MWCNTs). The polyurethane ionomer nanoparticles (PUI-NPs) were synthesized by emulsion polymerization, and could provide a good biocompatible microenvironment for Hb immobilization. The characteristic of (PUI-NPs)/MWCNTs and Hb/(PUI-NPs)/MWCNTs composite films were performed by using transmission electron microscopy (TEM), scanning electron microscopy (SEM), Fourier transform infrared (FT-IR) spectroscopy and circular dichroism (CD). Analytical results indicated that the immobilized Hb could maintain its native conformation in the (PUI-NPs)/MWCNTs hybrid film. Entrapped Hb in (PUI-NPs)/MWCNTs preserved its bioactivities and exhibited an excellent electrochemical behavior with a formal potential of −0.346 V in a pH 7.0 phosphate buffer. The formal potential of Hb varied linearly with the increase of pH in the range of 5.0–9.0 with a slope of 52.9 mV pH−1, indicating that one proton participated in the electrochemical reaction process. Moreover, the resulting biosensor displays an electrocatalytic activity to hydrogen peroxide (H2O2). The linear range for the determination of H2O2 was from 6.5 × 10−7 to 8.0 × 10−5 M with a detection limit of 2.4 × 10−7 M and a Michaelis–Menten constant Kmapp value of 0.155 mM. Consequently, our investigation demonstrated that the proposed method opens a way to develop biosensors by using polymer with good biocompatible in its nanostructured information.Graphical abstract.Highlights► PUI-NPs combined with MWCNTs to be the immobilization matrix for Hb. ► The linear range for the determination of H2O2 was from 2.0 × 10−7 to 8.0 × 10−5 M. ► The detection limit for determination of H2O2 was 1.2 × 10−7 M. ► The nanocomposite film can provide a favorable microenvironment for Hb to retain its bioactivity.

Preparation of polypropylene superhydrophobic surface and its blood compatibility

Co-reporter:Xiaomei Hou, Xiaobo Wang, Qinshu Zhu, Jianchun Bao, Chun Mao, Lancao Jiang, Jian Shen

Colloids and Surfaces B: Biointerfaces 2010 80(2) pp: 247-250

Publication Date(Web):

DOI:10.1016/j.colsurfb.2010.06.013

Evaluation of antithrombogenic and antibacterial activities of a graphite oxide/heparin–benzalkonium chloride composite

Co-reporter:Ninglin Zhou, Na Meng, Yinchen Ma, Xiangmin Liao, Jun Zhang, Li Li, Jian Shen

Carbon 2009 Volume 47(Issue 5) pp:1343-1350

Publication Date(Web):April 2009

DOI:10.1016/j.carbon.2009.01.025

A graphite oxide (GO)/heparin–benzalkonium chloride (C12) composite was synthesized. The composite was characterized by X-ray diffraction (XRD) and Fourier transform infrared spectroscopy (FTIR). XRD data showed that spacing between layers of GO increased from 0.59 to 3.256 nm. This enlarged layer spacing suggested that heparin–C12 complex intercalated completely in between layers of GO. FTIR also confirmed intercalation of heparin–C12 complex into GO gallery. In vitro release rate of heparin from GO–heparin–C12 was monitored for 30 days. Heparin released at a very fast rate from the composite matrix in the first day. The release slowed down significantly after the first day and continued for 30 days. In addition, antibacterial activity of the composite against Escherichia coli (E. coli) and Staphlococcus aureus (S. aureus) was evaluated using zone of inhibition and colony count assays. Both GO–heparin–C12 and GO–C12 clearly showed antibacterial activity against E. coli and S. aureus while GO alone has a relatively low activity against S. aureus and almost no effect on E. coli.

Novel Blood-Compatible Polyurethane Ionomer Nanoparticles

Co-reporter:Chun Mao, Lan-Cao Jiang, Wen-Ping Luo, Hong-Ke Liu, Jian-Chun Bao, Xiao-Hua Huang and Jian Shen

Macromolecules 2009 Volume 42(Issue 23) pp:9366-9368

Publication Date(Web):November 5, 2009

DOI:10.1021/ma901907t

Immobilization and direct electrochemistry of glucose oxidase on a tetragonal pyramid-shaped porous ZnO nanostructure for a glucose biosensor

Co-reporter:Zhihui Dai, Guojian Shao, Jianmin Hong, Jianchun Bao, Jian Shen

Biosensors and Bioelectronics 2009 Volume 24(Issue 5) pp:1286-1291

Publication Date(Web):1 January 2009

DOI:10.1016/j.bios.2008.07.047

A tetragonal pyramid-shaped porous ZnO (TPSP-ZnO) nanostructure is used for the immobilization, direct electrochemistry and biosensing of proteins. The prepared ZnO has a large surface area and good biocompatibility. Using glucose oxidase (GOD) as a model, this shaped ZnO is tested for immobilization of proteins and the construction of electrochemical biosensors with good electrochemical performances. The interaction between GOD and TPSP-ZnO is examined by using AFM, N2 adsorption isotherms and electrochemical methods. The immobilized GOD at a TPSP-ZnO-modified glassy carbon electrode shows a good direct electrochemical behavior, which depends on the properties of the TPSP-ZnO. Based on a decrease of the electrocatalytic response of the reduced form of GOD to dissolved oxygen, the proposed biosensor exhibits a linear response to glucose concentrations ranging from 0.05 to 8.2 mM with a detection limit of 0.01 mM at an applied potential of −0.50 V which has better biosensing properties than those from other morphological ZnO nanoparticles. The biosensor shows good stability, reproducibility, low interferences and can diagnose diabetes very fast and sensitively. Such the TPSP-ZnO nanostructure provides a good matrix for protein immobilization and biosensor preparation.

Modification of polyethylene with Pluronics F127 for improvement of blood compatibility

Co-reporter:Chun Mao, Cun Xia Liang, Yong Qiang Mao, Li Li, Xiao Mei Hou, Jian Shen

Colloids and Surfaces B: Biointerfaces 2009 Volume 74(Issue 1) pp:362-365

Publication Date(Web):1 November 2009

DOI:10.1016/j.colsurfb.2009.07.022

In order to improve blood compatibility of polyethylene (PE) film, the Pluronics F127 additives in the PE film were then crosslinked to be stably entrapped in the PE matrix. The crosslinking was done by free radicals produced from the decomposition of dicumyl peroxide (DCP) in the film through heating (120 °C). Surface properties of the Pluronics F127 additive-containing PE films were investigated by Fourier transform infrared spectroscopy (FTIR), electron spectroscopy for chemical analysis (ESCA) and water contact angle (WCA) measurements. The blood compatibility of the Pluronics F127 additive-containing films was evaluated by platelet-rich plasma and blood-cell adhesion tests, respectively. And the results were observed by scanning electron microscopy. The blood compatibility of the prepared Pluronics F127 additive-containing film is better than that of blank PE film. These results suggest that the blood compatibility of Pluronics F127 additive-containing films make them suitable biomaterials for some applications.

A novel tetragonal pyramid-shaped porous ZnO nanostructure and its application in the biosensing of horseradish peroxidase

Co-reporter:Zhihui Dai, Ke Liu, Yawen Tang, Xiaodi Yang, Jianchun Bao and Jian Shen

Journal of Materials Chemistry A 2008 vol. 18(Issue 16) pp:1919-1926

Publication Date(Web):10 Mar 2008

DOI:10.1039/B717794A

A novel nano-sized tetragonal pyramid-shaped porous ZnO (TPSP-ZnO) structure was, for the first time, prepared with a high morphological yield by a simple polyglycol-assisted wet chemical method. It was found that the polyglycol had a significant influence on the nucleation and pore formation in the ZnO nanostructure. The OH−concentration and the zinc counter-ion affected the morphology of the produced ZnO nanostructure. TPSP-ZnO can be used as an efficient matrix for immobilizing horseradish peroxidase (HRP) and applied to sense hydrogen peroxide (H2O2). Interestingly, it had better biosensing properties than solid spherical ZnO nanoparticles, which might result from the larger specific surface area of TPSP-ZnO, causing a higher HRP loading, and the tetragonal pyramid-shaped porous nanostructure having high fraction of surface atoms located on the corners and edges, resulting in an improved catalytic activity.

Molecular dynamics simulation study on zwitterionic structure to maintain the natural behavior of polyalanine13 in aqueous environment

Co-reporter:HaoMiao Zhu;Bo Li;Li Li

Science China Chemistry 2008 Volume 51( Issue 1) pp:78-85

Publication Date(Web):2008 January

DOI:10.1007/s11426-007-0119-4

Molecular dynamics simulations are applied to the initial stage of polyalanine13 conformational transition from α-helix to random coil in aqueous environment and the interaction of polyalanine13 with zwitterionic and hydrophobic surfaces respectively in the same condition. The analysis of secondary structure, hydrogen bonds, RMSD, dihedral distribution, and the degree of adsorption are performed. The results show that zwitterionic structure maintains the natural behavior of polyalanine13 in water to a better extent, which should be an indirect proof of the hypothesis of “maintain of normal structure.”

New biocompatible polypyrrole-based films with good blood compatibility and high electrical conductivity

Co-reporter:Chun Mao, Aiping Zhu, Qiong Wu, Xiangbai Chen, Jinheung Kim, Jian Shen

Colloids and Surfaces B: Biointerfaces 2008 Volume 67(Issue 1) pp:41-45

Publication Date(Web):15 November 2008

DOI:10.1016/j.colsurfb.2008.07.012

A novel O-butyryl chitosan (OBCS)-grafted polypyrrole (PPy) film was described. The immobilization was accomplished by photocrosslinking the OBCS onto PPy films under ultraviolet light irradiation. The surfaces of OBCS-grafted PPy film were characterized by attenuated total reflection Fourier transform infrared (ATR-FTIR) spectroscopy and electron spectroscopy for chemical analysis (ESCA). The blood compatibility of the OBCS-grafted PPy film was evaluated by platelet-rich plasma (PRP) contacting experiments and protein adsorption experiments in vitro. These results have demonstrated that the surface with immobilized OBCS shows much less platelet adhesive and fibrinogen adsorption compared to the control surface. The bulk conductivity values of PPy films were measured by a modified four-probe method. The composite films have both good blood compatibility and high electrical conductivity that make them suitable for using as potential biomaterials, such as electrically conducting blood vessel and functionally haemocompatible substrate of biosensor used directly in whole blood.

Thermooxidative aging and kinetics of the thermooxidative degradation of ethylene–propylene–diene terpolymer-graft-maleic anhydride/calcium carbonate composites

Co-reporter:Jun Zhang;Yong-Zhong Yao;Li Li;Ning-Lin Zhou;Ying-Xia Huang;Zhen-Mao Ma

Journal of Applied Polymer Science 2007 Volume 103(Issue 4) pp:2395-2401

Publication Date(Web):22 NOV 2006

DOI:10.1002/app.25376

We report the preparation of five different ethylene–propylene–diene terpolymer-graft-maleic anhydride/calcium carbonate samples with different calcium carbonate contents by precipitation and their mechanical properties and structural characterization.1 In this study, we tried to analyze the thermooxidative aging and the kinetics of thermooxidative degradation with retained tensile testing for various thermal-aging times at 150°C and with hot-loading-recovery measurements. The results of the hot-loading-recovery measurements and retained tensile testing showed that the sample with the best crosslinked structure had the best thermooxidative-aging resistance of all the samples. The experimental results for the thermooxidative degradation kinetics showed that the sample with the best crosslinked structure had the highest activation energy of thermal decomposition. © 2006 Wiley Periodicals, Inc. J Appl Polym Sci 103: 2395–2401, 2007

Waste polystyrene foam-graft-acrylic acid/montmorillonite superabsorbent nanocomposite

Co-reporter:Ping-Sheng Liu;Li Li;Ning-Lin Zhou;Jun Zhang;Shao-Hua Wei

Journal of Applied Polymer Science 2007 Volume 104(Issue 4) pp:2341-2349

Publication Date(Web):27 FEB 2007

DOI:10.1002/app.25714

A novel superabsorbent nanocomposite based on partially neutralized acrylic acid, waste polystyrene foam, and sodium type montmorillonite (Na-MMT) powder was synthesized through emulsion polymerization using N, N′-methylenebisacrylamide as a crosslinker, 2,2′-azo-bisiso-butyronitrile, ammonium persulfate, and sodium sulfite as mixed redox initiators. The effects of such factors as amount of Na-MMT, crosslinker, initiator, and neutralization degree on water absorbency of the superabsorbent were investigated. The composites were characterized by Fourier transform infrared spectroscope, X-ray diffraction, thermo gravimetric analysis, and scanning electron microscope. The results show that acrylic acid monomer successfully grafted onto the polystyrene chain, the layers of Na-MMT were exfoliated and dispersed in the composite at nano size after copolymerization. The introduction of waste polystyrene foam in the composite increased the water absorbency rate. The addition of Na-MMT not only enhanced the thermal stability of the composites but also increased its water absorbency, and the optimal water absorbencies of distilled water and saline water (w_NaCl = 0.9%) of the nanocomposites were more than 1180 g H₂O/g and 72.6 g H₂O/g, respectively. © 2007 Wiley Periodicals, Inc. J Appl Polym Sci 104: 2341–2349, 2007

Molecular dynamics simulation study on zwitterionic structure to maintain the normal conformations of Glutathione

Co-reporter:Han Yan;HaoMiao Zhu

Science China Chemistry 2007 Volume 50( Issue 5) pp:660-664

Publication Date(Web):2007 October

DOI:10.1007/s11426-007-0080-2

Molecular dynamics simulations were applied to normal conformational Glutathione (GSH) and GSH over zwitterionic and hydrophobic surfaces respectively. Conformational analysis of GSH during the simulation time on RMSD, conformational flexibility and dihedral distribution were performed. The results showed that zwitterionic structure maintains the normal conformations of GSH to a better extent, which should be a first good proof of the hypothesis of “maintain of normal structure”.

Synthesis and properties of a poly(acrylic acid)/montmorillonite superabsorbent nanocomposite

Co-reporter:Ping-Sheng Liu;Li Li;Ning-Lin Zhou;Jun Zhang;Shao-Hua Wei

Journal of Applied Polymer Science 2006 Volume 102(Issue 6) pp:5725-5730

Publication Date(Web):28 SEP 2006

DOI:10.1002/app.25090

A novel superabsorbent nanocomposite was synthesized through the intercalation polymerization of partially neutralized acrylic acid and a sodium-type montmorillonite powder with N,N′-methylenebisacrylamide as a crosslinker and ammonium persulfate and sodium sulfite as a type of mixed redox initiator. The effects of such factors as the amounts of the sodium-type montmorillonite, crosslinker, and initiator and neutralization degree on the water absorbency of the nanocomposite were investigated. The structure and micrographs of the superabsorbent were characterized with Fourier transform infrared spectroscopy, X-ray diffraction, and scanning electron microscopy. The results showed that the acrylic acid monomer was successfully intercalated into the montmorillonite layers and banded together with them. The montmorillonite layers were exfoliated and basically dispersed in the composite on a nanoscale after the polymerization. The water absorbency of the nanocomposite was much higher than that of pure poly(acrylic acid). The optimum absorbency of the nanocomposite in distilled water and saline water (NaCl concentration = 0.9%) was 1201 and 83 g/g, respectively. © 2006 Wiley Periodicals, Inc. J Appl Polym Sci 102: 5725–5730, 2006

Amino-terminated poly(ethylene glycol) as the initiator for the ring-opening polymerization of 3-methylmorpholine-2,5-dione

Co-reporter:Weiwei Tian, Qiang Chen, Chunhong Yu, Jian Shen

European Polymer Journal 2003 Volume 39(Issue 9) pp:1935-1938

Publication Date(Web):September 2003

DOI:10.1016/S0014-3057(03)00091-0

Block copolymers with poly[3-methylmorpholine-2,5-dione] (PMMD) and poly[ethylene glycol] (PEG) blocks, PMMD-b-PEG-b-PMMD, were synthesized via ring-opening polymerization of 3-methylmorpholine-2,5-dione with amino-terminated PEG as the initiator at 140 °C within 10 h. Three kind of amino terminated PEG with different average molecular weight were used. The block copolymer was amorphous and the glass transition temperature decreased with increase of PEG block in the copolymer.

Synthesis and antifungal activities of polymer/montmorillonite–terbinafine hydrochloride nanocomposite films

Co-reporter:Na Meng, Ning-Lin Zhou, Shuang-Quan Zhang, Jian Shen

Applied Clay Science (October 2009) Volume 46(Issue 2) pp:136-140

Publication Date(Web):October 2009

DOI:10.1016/j.clay.2009.07.003

Synthesis and antimicrobial activities of polymer/montmorillonite–chlorhexidine acetate nanocomposite films

Co-reporter:Na Meng, Ning-Lin Zhou, Shuang-Quan Zhang, Jian Shen

Applied Clay Science (January 2009) Volume 42(Issues 3–4) pp:667-670

Publication Date(Web):January 2009

DOI:10.1016/j.clay.2008.06.016

Montmorillonite–phosphatidyl choline/PDMS films: A novel antithrombogenic material

Co-reporter:Ninglin Zhou, Su Fang, Dong Xu, Jun Zhang, Hong Mo, Jian Shen

Applied Clay Science (December 2009) Volume 46(Issue 4) pp:401-403

Publication Date(Web):December 2009

DOI:10.1016/j.clay.2009.10.012

Label-free immunosensor based on hyperbranched polyester for specific detection of α-fetoprotein

Co-reporter:Yanlian Niu, Tian Yang, Shangshang Ma, Fang Peng, Meihui Yi, Mimi Wan, Chun Mao, Jian Shen

Biosensors and Bioelectronics (15 June 2017) Volume 92() pp:

Publication Date(Web):15 June 2017

DOI:10.1016/j.bios.2017.01.069

•The multi-functional hyperbranched polyester nanoparticles were synthesized through a simple one-step chemical reaction.•HBPE-NO2 nanoparticles were first developed to construct a label-free immunosensor for specific detection of α-fetoprotein.•The immunosensor showed excellent electrochemical properties owing to the multi-functional properties of HBPE-NO2 nanoparticles, such as nanosized structure, high density end-groups, unique chemical reactivity.A novel label-free immunosensor based on hyperbranched polyester nanoparticles with nitrite groups (HBPE-NO2), which were synthesized through a simple one-step chemical reaction, was first developed for specific detection of α-fetoprotein (AFP), the tumor marker for liver cancer. The obtained HBPE-NO2 nanoparticles (NPs) were characterized by the proton nuclear magnetic resonance spectroscopy (1H NMR), X-ray photoelectron spectroscopy (XPS) and X-ray diffraction (XRD). And the fabricated process of immunosensor was investigated by attenuated total reflection Fourier-transform infrared spectra (ATR-FTIR), static water contact angles, scanning electron microscope (SEM), cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). The electrochemical performances of the AFP immunosensor were studied. Results indicated the prepared HBPE-NO2-modified immunosensor showed excellent electrochemical properties and satisfactory accuracy for the detection of AFP of the real clinical samples that attributed to the properties of the HBPE-NO2 NPs, which had nanosized structure to increase the specific surface area and unique chemical reactivity for loading capacity of protein molecules. Construction of biosensors using the structure and properties of hyperbranched molecules will offer ideal electrode substrates, which provided more possibilities for the design of biosensor.

Controlled release and antibacterial activity chlorhexidine acetate (CA) intercalated in montmorillonite

Co-reporter:Na Meng, Ning-Lin Zhou, Shuang-Quan Zhang, Jian Shen

International Journal of Pharmaceutics (1 December 2009) Volume 382(Issues 1–2) pp:45-49

Publication Date(Web):1 December 2009

DOI:10.1016/j.ijpharm.2009.08.004

The aim of this study was to prepare chlorhexidine acetate (CA)/montmorillonite intercalation composites and its antibacterial potential was evaluated with pathogenic bacteria, Staphylococcus aureus and Pseudomonas aeruginosa. The CA/MMT was characterized by X-ray diffraction (XRD), Fourier transformed infrared (FT-IR), and thermogravimetric analysis (TGA). CA was successfully intercalated into the interlayer of MMT and in vitro release properties of the intercalated CA have been investigated in phosphate buffered saline media (pH 7.4) at 37 ± 0.5 °C. At drug release study, CA showed initial burst effect for 24 h and then continuously released for 72 h. Their antibacterial activity was assayed by the inhibitory zone method. The CA/MMT was tested for antimicrobial activity against S. aureus and P. aeruginosa. The CA/MMT strongly inhibited the growth of a wide variety of microorganisms, including Gram-positive bacteria, Gram-negative bacteria.

Preparation, blood compatibility and anticoagulant effect of heparin-loaded polyurethane microspheres

Co-reporter:Fengyu Tong, Xiaoqiang Chen, Libin Chen, Peiyuan Zhu, Jianfeng Luan, Chun Mao, Jianchun Bao and Jian Shen

Journal of Materials Chemistry A 2013 - vol. 1(Issue 4) pp:NaN453-453

Publication Date(Web):2012/11/05

DOI:10.1039/C2TB00250G

A novel tetragonal pyramid-shaped porous ZnO nanostructure and its application in the biosensing of horseradish peroxidase

Co-reporter:Zhihui Dai, Ke Liu, Yawen Tang, Xiaodi Yang, Jianchun Bao and Jian Shen

Journal of Materials Chemistry A 2008 - vol. 18(Issue 16) pp:NaN1926-1926

Publication Date(Web):2008/03/10

DOI:10.1039/B717794A

Preparation of a novel immunosensor for tumor biomarker detection based on ATRP technique

Co-reporter:Xiaobo Wang, Min Zhou, Yinyan Zhu, Jingjing Miao, Chun Mao and Jian Shen