Xiaojuan Li

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Organization: Fuzhou University
Department: College of Environment and Resources
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Co-reporter:Guixiao Jin, Chunmei Wang, Linlin Yang, Xiaojuan Li, Longhua Guo, Bin Qiu, Zhenyu Lin, Guonan Chen
Biosensors and Bioelectronics 2015 Volume 63() pp:166-171
Publication Date(Web):15 January 2015
DOI:10.1016/j.bios.2014.07.033
•An ECL biosensor for thrombin was proposed.•The sensor combines the advantages of HRCA, aptamer and ECL.•The detection limits is 1.2 aM and the linear range is 3.0–300 aM.•The sensor has high selectivity of thrombin.An ultrasensitive electrochemiluminescence (ECL) aptamer sensor for protein (thrombin as an example) detection based on hyperbranched rolling circle amplification (HRCA) had been developed. A complementary single-strand DNA (CDNA) of the thrombin aptamer had been modified on the gold electrode firstly, and then hybridized with thrombin aptamer to make the aptamer immobilized on the electrode surface, in the presence of thrombin, aptamer–thrombin bioaffinity complexes formed and made thrombin aptamer leave the electrode surface. Thus, the linear padlock probe hybridized with the free CDNA on the electrode surface and circularized by Escherichia coli   DNA ligase. Subsequently, the linear padlock probe was served as a template for the initiation of HRCA reaction, and a lot of dsDNA modified on the electrode surface. Then Ru(phen)32+Ru(phen)32+ (acted as the ECL indicator) intercalates specifically into double-stranded DNA (dsDNA) grooves to generate ECL signal. The ECL intensity of the system has a linear relationship with thrombin concentration in the range of 3.0–300 aM with a detection limit of 1.2 aM (S/N=3). The proposed method combines the high sensitivity of ECL, exponential amplification of HRCA for signal enhancement and high selectivity of aptamer.
Co-reporter:Xiaojuan Li, Duanlian Tang, Fan Tang, Yunyan Zhu, Changfa He, Minghua Liu, Chunxiang Lin, Yifan Liu
Materials Research Bulletin 2014 56() pp: 125-133
Publication Date(Web):
DOI:10.1016/j.materresbull.2014.05.013
Co-reporter:Xiaojuan Li;Lingyan Zheng;Lianzhu Huang;Ou Zheng;Zhenyu Lin;Longhua Guo;Bin Qiu;Guonan Chen
Journal of Applied Polymer Science 2013 Volume 129( Issue 5) pp:2857-2864
Publication Date(Web):
DOI:10.1002/app.39009

Abstract

The potential application of copper coordination polymer with dithiooxamide (H2dtoaCu), one of the metal-organic frameworks (MOFs) in the adsorption removal of crystal violet (CV) from aqueous solution was studied. Batch adsorption experiments were carried out as a function of initial pH, adsorbent concentration, contact time, initial dye concentration and temperature. The adsorption of CV on H2dtoaCu can be best described by the Langmuir isotherm model (R2 > 0.9983) with maximum monolayer adsorption capacity of 165.83, 185.87, and 204.50 mg g−1 at 298, 308, and 318 K, respectively. The kinetics of CV adsorption followed pseudo-second-order model (R2 > 0.9984) and the chemisorption was proved to be rate-limiting step. Thermodynamic parameters, the change of free energy (ΔG°), entropy (ΔS°) and enthalpy (ΔH°), showed that the adsorption of CV on H2dtoaCu was feasible, spontaneous and endothermic process. Here we concluded that H2dtoaCu is a promising adsorbent for the removal of harmful dyes from aqueous solution. © 2013 Wiley Periodicals, Inc. J. Appl. Polym. Sci., 2013

Ruthenium(2+),tris(1,10-phenanthroline-kN1,kN10)-, dichloride, monohydrate,(OC-6-11)- (9CI)
Ruthenium(2+),tris(2,2'-bipyridine-kN1,kN1')-, (OC-6-11)-
Copper bromide
Thrombin