Co-reporter:Wenwen Chen, Fengjing Cao, Wenshu Zheng, Yue Tian, Yunlei Xianyu, Peng Xu, Wei Zhang, Zhuo Wang, Ke Deng and Xingyu Jiang
Nanoscale 2015 vol. 7(Issue 5) pp:2042-2049
Publication Date(Web):15 Dec 2014
DOI:10.1039/C4NR06726F
We report a method for rapid, effective detection of both Cr(III) and Cr(VI) (in the form of Cr3+ and Cr2O72−, the main species of chromium in the natural environment) by making use of meso-2,3-dimercaptosuccinic acid (DMSA)-functionalized gold nanoparticles (Au NPs). The limit of detection (LOD) is 10 nM with the naked eye and the assay can be applied in detecting chromium in polluted soil from Yun-Nan Province in Southwest China. We use density functional theory to calculate the change of the Gibbs free energy (ΔG) of the interactions between the DMSA-Au NP system and various metal ions, which shows that DMSA-Au NPs have high specificity for both Cr3+ and Cr2O72−.
Co-reporter:Lu Zhang, Wenwen Liu, Xianhong Huang, Guanxin Zhang, Xuefei Wang, Zhuo Wang, Deqing Zhang and Xingyu Jiang
Analyst 2015 vol. 140(Issue 17) pp:5849-5854
Publication Date(Web):19 Jun 2015
DOI:10.1039/C5AN00918A
Here, we explore the new application of an old molecule. We find that the tetraphenylethene-indolium molecule (TPE-indo) can both image the mitochondria (in the aggregated state), and indicate mitochondrial activity by the fluorescence change of TPE-indo. TPE-indo shows good photostability, longer emission wavelength, targeting effect for mitochondria, and better response to the changes of the mitochondrial membrane potential (ΔΨm).
Co-reporter:Wenwen Chen, Yuexiao Jia, Yan Feng, Wenshu Zheng, Zhuo Wang and Xingyu Jiang
RSC Advances 2015 vol. 5(Issue 76) pp:62260-62264
Publication Date(Web):14 Jul 2015
DOI:10.1039/C5RA09099G
We synthesized ionic liquid (1-ethyl-3-methylimidazolium thiocyanate)-coated gold nanoparticles (IL-Au NPs) using a one-pot method and introduced them in the colorimetric assay for Al3+. IL-Au NPs showed good sensitivity and selectivity. They can determine the concentration of aluminum in vermicelli samples.
Co-reporter:Yongming Guo, Yi Zhang, Huawu Shao, Zhuo Wang, Xuefei Wang, and Xingyu Jiang
Analytical Chemistry 2014 Volume 86(Issue 17) pp:8530
Publication Date(Web):August 13, 2014
DOI:10.1021/ac502461r
A simple and label-free colorimetric method for cadmium ions (Cd2+) detection using unmodified gold nanoparticles (AuNPs) is reported. The unmodified AuNPs easily aggregate in a high concentration of NaCl solution, but the presence of glutathione (GSH) can prevent the salt-induced aggregation of AuNPs. When Cd2+ is added to the stable mixture of AuNPs, GSH, and NaCl, Cd2+ can coordinate with 4× GSH as a spherical shaped complex, which decreases the amount of free GSH on the surface of gold nanoparticles to weaken the stability of AuNPs, and AuNPs will easily aggregate in high-salt conditions. On the basis of the mechanism, we design a simple, label-free colorimetric method using AuNPs accompanied by GSH in a high-salt environment to detect Cd2+ in water and digested rice samples.
Co-reporter:Wen-Wen CHEN, Yong-Ming GUO, Wen-Shu ZHENG, Yun-Lei XIANYU, Zhuo WANG, Xing-Yu JIANG
Chinese Journal of Analytical Chemistry 2014 Volume 42(Issue 3) pp:307-314
Publication Date(Web):March 2014
DOI:10.1016/S1872-2040(13)60714-8
Biochemical analysis assays based on colorimetric methods using gold nanoparticles have many advantages such as high sensitivity, good selectivity, naked-eyes readout and complex instruments free. These methods have good prospects in applications. This review mainly focuses on colorimetric assays applying gold nanoparticles for biomolecules detection.The determination (colorimetric and fluorometric) of AChE is based on RB-AuNPs. The well-dispersed RB-AuNPs (red) are induced to aggregate (purple) via electrostatic interaction in the presence of thiocholine derived from the hydrolysis of ATC catalyzed by AChE in the CSF of transgenic mice, accompanied with the fluorescence recovery of RB (the color of the stars changed from gray to green).
Co-reporter:Wenwen Chen; Qizhai Li;Wenshu Zheng;Fang Hu; Guanxin Zhang; Zhuo Wang; Deqing Zhang; Xingyu Jiang
Angewandte Chemie International Edition 2014 Volume 53( Issue 50) pp:13734-13739
Publication Date(Web):
DOI:10.1002/anie.201407606
Abstract
We report a method for the rapid and efficient identification of bacteria making use of five probes having fluorescent characteristics (F-array) and subsequent statistical analysis. Eight kinds of bacteria, including normal and multidrug-resistant bacteria, are differentiated successfully. Our easy-to-perform and time-saving method consists of mixing bacteria and probes, recording fluorescent intensity data by automated flow cytometry, and statistical analysis. No washing steps are required in order to identify the different bacteria simultaneously.
Co-reporter:Wenwen Chen; Qizhai Li;Wenshu Zheng;Fang Hu; Guanxin Zhang; Zhuo Wang; Deqing Zhang; Xingyu Jiang
Angewandte Chemie 2014 Volume 126( Issue 50) pp:13954-13959
Publication Date(Web):
DOI:10.1002/ange.201407606
Abstract
We report a method for the rapid and efficient identification of bacteria making use of five probes having fluorescent characteristics (F-array) and subsequent statistical analysis. Eight kinds of bacteria, including normal and multidrug-resistant bacteria, are differentiated successfully. Our easy-to-perform and time-saving method consists of mixing bacteria and probes, recording fluorescent intensity data by automated flow cytometry, and statistical analysis. No washing steps are required in order to identify the different bacteria simultaneously.
Co-reporter:Yunlei Xianyu, Kui Zhu, Wenwen Chen, Xuefei Wang, Hongmei Zhao, Jiashu Sun, Zhuo Wang, and Xingyu Jiang
Analytical Chemistry 2013 Volume 85(Issue 15) pp:7029
Publication Date(Web):July 24, 2013
DOI:10.1021/ac401925j
We report an ultrasensitive and colorimetric assay for Cu(II) via enzymatic amplification strategy. The enzymatic activity of horseradish peroxidase (HRP) is strongly inhibited by Cu(I), which can be used indirectly to assay Cu(II). The limit of detection (LOD) is 0.37 nM, and the detection of 20 nM Cu(II) in solution can be achieved with naked eyes. This assay can be used to construct a colorimetric logic gate.
Co-reporter:Dingbin Liu;Wenwen Chen;Yue Tian;Sha He;Wenfu Zheng;Jiashu Sun;Xingyu Jiang
Advanced Healthcare Materials 2012 Volume 1( Issue 1) pp:90-95
Publication Date(Web):
DOI:10.1002/adhm.201100002
Co-reporter:Dingbin Liu, Wenwen Chen, Jinhua Wei, Xuebing Li, Zhuo Wang, and Xingyu Jiang
Analytical Chemistry 2012 Volume 84(Issue 9) pp:4185
Publication Date(Web):April 5, 2012
DOI:10.1021/ac300545p
This report presents a highly sensitive, rhodamine B-covered gold nanoparticle (RB-AuNP) -based assay with dual readouts (colorimetric and fluorometric) for detecting organophosphorus and carbamate pesticides in complex solutions. The detection mechanism is based on the fact that these pesticides can inhibit the activity of acetylcholinesterase (AChE), thus preventing the generation of thiocholine (which turns the RB-AuNP solutions blue and unquenches the fluorescence of RB simultaneously). The color of the RB-AuNP solution remains red and the fluorescence of RB remains quenched. By use of this dual-readout assay, the lowest detectable concentrations for several kinds of pesticides including carbaryl, diazinon, malathion, and phorate were measured to be 0.1, 0.1, 0.3, and 1 μg/L, respectively, all of which are much lower than the maximum residue limits (MRL) as reported in the European Union pesticides database as well as those from the U.S. Department Agriculture (USDA). This assay allows detection of pesticides in real samples such as agricultural products and river water. The results in detecting pesticide residues collected from food samples via this method agree well with those from high-performance liquid chromatography (HPLC). This simple assay is therefore suitable for sensing pesticides in complex samples, especially in combination with other portable platforms.
Co-reporter:Kui Zhu, Yi Zhang, Sha He, Wenwen Chen, Jianzhong Shen, Zhuo Wang, and Xingyu Jiang
Analytical Chemistry 2012 Volume 84(Issue 10) pp:4267
Publication Date(Web):April 27, 2012
DOI:10.1021/ac3010567
This letter presents a click-chemistry-based assay for proteins (CAP) that allows quantitative determination of the concentration of proteins, using azide- and alkyne-functionalized gold nanoparticles (AuNPs). Compared with conventional methods, CAP has a broader linear range for detection of proteins with good selectivity. CAP enables the analysis of total proteins in various sera and milk samples.
Co-reporter:Yongming Guo, Zhuo Wang, Huawu Shao and Xingyu Jiang
Analyst 2012 vol. 137(Issue 2) pp:301-304
Publication Date(Web):03 Nov 2011
DOI:10.1039/C1AN15877E
A simple and one-pot method for the synthesis of water-soluble, red-emitting, highly fluorescent gold nanoparticles has been reported using 11-mercaptoundecanoic acid (11-MUA) as the protecting group. We found that the fluorescent gold nanoparticles could selectively detect copper ions in aqueous solution, with a limit of detection of about 87 nM.
Co-reporter:Yingyi Liu;Jie Yu;Wenwen Chen;Dingbin Liu;Xingyu Jiang
Chinese Journal of Chemistry 2012 Volume 30( Issue 9) pp:2047-2051
Publication Date(Web):
DOI:10.1002/cjoc.201200655
Abstract
We have developed a microfluidic chip for colorimetric Cu2+ detection. In this chip, it is facile to do colorimetric Cu2+ detection based on gold nanoparticles. This method has a dynamic detection range from 0.75 to 50 µmol/L with only 20 µL solution including detection reagents and sample. The result can be readout by naked eye and photographed by digital cameras. With the help of image processing software, we could measure the RGB value and calculate the Blue/Red ratio for more accurate quantification. Tap water could be detected in this portable chip.
Co-reporter:Yingyi Liu;Jie Yu;Meihong Du;Wenjun Wang;Wei Zhang
Biomedical Microdevices 2012 Volume 14( Issue 1) pp:17-23
Publication Date(Web):2012 February
DOI:10.1007/s10544-011-9581-z
This paper describes a vacuum-accelerated microfluidic immunoassay (we abbreviate it as VAMI) by sandwiching a filter membrane between a two-layer chip. A direct assay of IgG demonstrated that VAMI could simultaneously achieve higher sensitivity and require less time compared with conventional microfluidic immunoassays. We further applied VAMI to carry out a 3-step competitive assay (including antigen immobilization, competitive reaction and 2nd antibody reaction) for detecting the illegal food additive Sudan Red. A total assay time of 15 min with a limit of detection (LOD) of 1 ng ml-1 is achieved.
Co-reporter:Dingbin Liu, Zhuo Wang and Xingyu Jiang
Nanoscale 2011 vol. 3(Issue 4) pp:1421-1433
Publication Date(Web):28 Feb 2011
DOI:10.1039/C0NR00887G
In recent years, gold nanoparticles (AuNPs) have drawn considerable research attention in the fields of catalysis, drug delivery, imaging, diagnostics, therapy and biosensors due to their unique optical and electronic properties. In this review, we summarized recent advances in the development of AuNP-based colorimetric and fluorescent assays for ions including cations (such as Hg2+, Cu2+, Pb2+, As3+, Ca2+, Al3+, etc) and anions (such as NO2−, CN−, PF6−, F−, I−, oxoanions), and small organic molecules (such as cysteine, homocysteine, trinitrotoluene, melamine and cocaine, ATP, glucose, dopamine and so forth). Many of these species adversely affect human health and the environment. Moreover, we paid particular attention to AuNP-based colorimetric and fluorescent assays in practical applications.
Co-reporter:Tangsong Li, Kui Zhu, Sha He, Xi Xia, Shaoqin Liu, Zhuo Wang and Xingyu Jiang
Analyst 2011 vol. 136(Issue 14) pp:2893-2896
Publication Date(Web):24 May 2011
DOI:10.1039/C1AN15256D
We developed a simple, non-enzymatic approach for the colorimetric detection of glucose based on a gold nanoparticles (Au NPs) assisted silver mirror reaction (AuSMR). The linear range of the concentration of glucose is from 0.04 mM to 1 mM, and the lowest concentration that can be distinguished by the naked eye is 10 nM. This approach has been successfully used for detecting glucose in serum.
Co-reporter:Kai Cui, Zhenling Chen, Zhuo Wang, Guanxin Zhang and Deqing Zhang
Analyst 2011 vol. 136(Issue 1) pp:191-195
Publication Date(Web):06 Oct 2010
DOI:10.1039/C0AN00456A
A resorufin derivative with a DBS group (probe 1) was designed and investigated for the detection of acetylcholinesterase (AChE) and inhibitor screening. The new assay is based on cascade enzymatic and chemical reactions of ATC, AChE and probe 1, and it can be carried out in a dual-signal detection mode. Moreover, the results show that probe 1 can be used for cell fluorescence staining.
Co-reporter:WenWen Chen;TangSong Li;Sha He;DingBin Liu
Science China Chemistry 2011 Volume 54( Issue 8) pp:
Publication Date(Web):2011 August
DOI:10.1007/s11426-011-4338-3
Immunoassays are useful for many bioassays. Many new techniques and materials are introduced into the immunoassay to improve the efficiency. This paper reviews recent progress in the application of microfluidic systems and gold nanoparticles in immunoassay. The micro/nano technologies and materials can offer good sensitivity, fast detection, cost-effectiveness and easy signal readout. In particular, the miniaturization of microfluidics and colorimetric assays based on gold nanoparticles have dramatically improved the efficiency of immunoassays.
Co-reporter:Yongming Guo, Zhuo Wang, Weisi Qu, Huawu Shao, Xingyu Jiang
Biosensors and Bioelectronics 2011 Volume 26(Issue 10) pp:4064-4069
Publication Date(Web):15 June 2011
DOI:10.1016/j.bios.2011.03.033
A simple, cost-effective and rapid colorimetric method for any or all of Hg2+, Pb2+ and Cu2+ detection using papain-functionalized gold nanoparticles (P-AuNPs) has been developed. Papain is a protein with seven cystein residues, which can selectively bind with Hg2+, Pb2+ and Cu2+. We functionalized gold nanoparticles with papain. The P-AuNPs could be used to simultaneously detect Hg2+, Pb2+ and Cu2+, and showed different responses to the three ions in an aqueous solution based on the aggregation-induced color change of gold nanoparticles. The P-AuNPs displayed the most obvious response to mercury ions in water in contrast to lead and copper ions, and the real water sample analysis verified the conclusion. The sensitivity of the detection system was influenced by the pH of the P-AuNPs solution, the concentration of P-AuNPs and the size of gold nanoparticles, and we found that larger gold nanoparticles contributed to more sensitive results. The detection system can detect as low as 200 nM Hg2+, Pb2+ or Cu2+ using 42 nm gold nanoparticles. We expect our approach to have wide-ranging applications in the developing region for monitoring water quality in some areas.
Co-reporter:Dingbin Liu;Wenwen Chen;Kang Sun; Ke Deng; Wei Zhang; Zhuo Wang; Xingyu Jiang
Angewandte Chemie 2011 Volume 123( Issue 18) pp:4189-4193
Publication Date(Web):
DOI:10.1002/ange.201008198
Co-reporter:Weisi Qu;Yingyi Liu;Dingbin Liu; Zhuo Wang; Xingyu Jiang
Angewandte Chemie International Edition 2011 Volume 50( Issue 15) pp:3442-3445
Publication Date(Web):
DOI:10.1002/anie.201006025
Co-reporter:Dingbin Liu;Wenwen Chen;Kang Sun; Ke Deng; Wei Zhang; Zhuo Wang; Xingyu Jiang
Angewandte Chemie International Edition 2011 Volume 50( Issue 18) pp:4103-4107
Publication Date(Web):
DOI:10.1002/anie.201008198
Co-reporter:Weisi Qu;Yingyi Liu;Dingbin Liu; Zhuo Wang; Xingyu Jiang
Angewandte Chemie 2011 Volume 123( Issue 15) pp:3504-3507
Publication Date(Web):
DOI:10.1002/ange.201006025
Co-reporter:Kang Sun, Lusheng Song, Yunyan Xie, Dingbin Liu, Dong Wang, Zhuo Wang, Wanshun Ma, Jinsong Zhu, and Xingyu Jiang
Langmuir 2011 Volume 27(Issue 10) pp:5709-5712
Publication Date(Web):April 26, 2011
DOI:10.1021/la2012099
We report a one-step, mild method to modify antifouling oligo(ethylene glycol)-terminated self-assembled monolayers. We demonstrate for the first time that self-polymerized dopamine, previously reported as an underwater adhesive, can be patterned on typical antifouling surfaces by microfluidic patterning or microcontact printing. The patterns can be applied in spatiotemporal cell patterning.
Co-reporter:Yuyun Zhao, Zhuo Wang, Wei Zhang and Xingyu Jiang
Nanoscale 2010 vol. 2(Issue 10) pp:2114-2119
Publication Date(Web):09 Aug 2010
DOI:10.1039/C0NR00309C
This report shows that, of the Tween series (Tween 20, Tween 40, Tween 60 and Tween 80) of nonionic surfactants adsorbed on gold nanoparticles (NPs), Tween 80 makes the NPs most stably dispersed in aqueous solutions with or without the presence of representative biological molecules, such as nucleic acids or proteins of different sizes, isoelectric points (pIs) and shapes. In addition, the stability of gold NPs already modified with poly(L-lysine)-graft-poly(ethylene glycol) (PLL-PEG) or hexa(ethylene glycol)-terminated undecanylthiol (HS(CH2)11EG6OH) is further improved in solutions of proteins when Tween 80 is co-adsorbed on the gold NPs. This strategy is the most effective when adsorption of Tween 80 on gold NPs precedes the coating of PLL-PEG or HS(CH2)11EG6OH on the NPs.
Co-reporter:Dingbin Liu, Weisi Qu, Wenwen Chen, Wei Zhang, Zhuo Wang, and Xingyu Jiang
Analytical Chemistry 2010 Volume 82(Issue 23) pp:9606
Publication Date(Web):November 11, 2010
DOI:10.1021/ac1021503
We provide a highly sensitive and selective assay to detect Hg2+ in aqueous solutions using gold nanoparticles modified with quaternary ammonium group-terminated thiols at room temperature. The mechanism is the abstraction of thiols by Hg2+ that led to the aggregation of nanoparticles. With the assistance of solar light irradiation, the detection limit can be as low as 30 nM, which satisfies the guideline concentration of Hg2+ in drinking water set by the WHO. In addition, the dynamic range of detection is wide (3 × 10−8−1 × 10−2 M). This range, to our best knowledge, is the widest one that has been reported so far in gold nanoparticle (AuNP)-based assays for Hg2+.
